Wednesday, April 21, 2021

ANTI BACTERIAL ACTIVITY OF Cordia dichotoma G. FORST. BARK HEXANE EXTRACT AND IDENTIFICATION OF ITS CONSTITUENTS BY GC-MS ANALYSIS | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Cordia dichotoma is the target. G. Forst is a member of the Boraginaceae family, which is known for its biological activities. Ulcers have been treated with powdered plant stem bark. Antimicrobial and anti-implantation properties are found in the stem bark. The aim of this study is to use GC-MS to determine the phytoconstituents of stem bark hexane extract and to investigate its antibacterial activity. Methods: Cold and hot hexane solvents were used to remove the powdered stem bark, and the solvent was then distilled to determine its constituents using GCMS. Saponification, column chromatography, and preparative TLC were used to purify the crude hexane. GCMS was used to evaluate the fractions obtained from the above-mentioned methods. The disc diffusion method was used to test the hexane extract for antibacterial activity against two bacterial pathogens. Based on peak area percentage, retention time, and structure, eleven components from crude hexane extract, eight components from Fraction 16c, and three components from Fraction 16d were classified using GCMS.



Please see the link - https://www.ikprress.org/index.php/PCBMB/article/view/5666

AN OVERVIEW AND VISUALISATION OF RESEARCH TRENDS IN LATE BLIGHT DISEASE OF PLANT | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 The aim of this article was to compile the literature on late blight and to discover trends in the field. Plant Disease is the journal with the most articles published. The journal with the most citations and co-authorship links is Molecular Plant-Microbe Interactions. Nature has the highest total number of citations. The United States is the most active country, and Luxembourg is the most profitable country in terms of average citations. With the most articles and co-authorship ties, Washington State University is the most active organisation. The organisations with the most citations were the Laboratory of Phytopathology and Wageningen University. Plant Research International has the highest average citation per paper, making it the most active organisation. Fry. W.E. is the most prolific author, with the most publications; Knowles N.R. has the most co-authorship links; and Kamoun S has the most cumulative citations. Cano L.M. is the most productive author with the highest average citation.


Please see the link - https://www.ikprress.org/index.php/PCBMB/article/view/5665

EFFECT OF DIFFERENT CHEMICAL TREATMENTS ON WEEDS, YIELD OF SOFT WHEAT AND YIELD COMPONENTS | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Autumn cereals need chemical weed control in order to produce a good yield. During the agricultural companion 2015-2016, we tested the effect of eight herbicide treatments on soft wheat yield Triticum aestivum and its components at the INRA-Allal Tazi experimental station in the Gharb Region (Morocco). Herbicides, both single and mixed, were used as therapies. The adventitious flora is described by 26 species belonging to 14 botanical families, according to the station's inventory. Furthermore, dicotyledons account for 92.31 percent of the total, while therophytes account for 88.46 percent. Our findings revealed that all herbicide treatments were successful. When compared to control, grain yield and part (number of wheat feet per m2, number of wheat ears per m2, biomass (stem+ ears), and weight of 1000 grains) increased significantly. The mesosulfuron methyl + iodosulfuron methyl treatment was the most successful against weeds among the simple treatments. With 50.8 qt/ha, this treatment produced the highest grain yield. As compared to other combined treatments, plots treated with 2,4-D+ MCPA + Clodinafoppropargyl + pendimethaline (Pr+Tp+Pl) and 2,4-D+MCPA+Clodinafoppropargyl+ Prosulfocarbe (Pr+Tp+Bo) produced the highest yield of 52 qt/ha.



Please see the link - https://www.ikprress.org/index.php/PCBMB/article/view/5664

FORMATION OF HARD SPRING WHEAT PRODUCTIVITY DEPENDING ON THE MINERAL FERTILIZERS AND BIOLOGOCAL PREPARATIONS| PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Introduction: In the face of erratic and inadequate moisture, it is important to research the fundamental elements of hard spring wheat technology in the light of climate change. Field, laboratory, assessment, and comparative methods were used. The application of microbiological biological preparations based on the history of mineral nutrition in the technologies of growing hard spring wheat was studied and analysed in depth. Plant leaf surface formation was influenced by both the context of mineral nutrition and the use of microbiological preparations. For the use of polymyxacarbonate, seed treatment with biopreparations increased the area of the leaf surface by 20.3 percent on non-fertilized areas. 20.5 percent for diazophyte and 23.9 percent for a combination of the two. It was 31.9 thousand m2 / ha for fertilisers N45R45K30, 19.1 percent more for polymicobacterin, 13.8 percent more for diazophyte, and 24.1 percent more for a mixture of preparations. Since the area of the leaf surface was very high (29.2 thousand m2 / ha), the fertilised history of "straw N10 per tonne of by-products" was successful, and with the use of biopreparations, respectively, 33.9; 32.9; 36.4 thousand m2 / ha. The number of viable stems per unit area and the weight of grain from one ear decide the yield of hard spring wheat. The highest yield was in areas planted with polymicobacterium and diazophyte inoculated seeds on the fertiliser background N45R45K30 - 3.50 t / ha, which is 1.47 t / ha (72.4 percent) higher than plots without mineral fertilisers and biopreparations (2.03 t / ha). Applying fertiliser to hard spring wheat is a sensible way to boost yields. With the mandatory treatment of seeds before sowing with a mixture of biological products ("N10 straw per tonne of by-products"), grain yield was 3.16 t/ha, which is 1.13 t/ha (55.7 percent) higher than non-fertilized cultivation. Conclusions: In conditions of unstable moisture, it is important to apply pre-sowing seed treatment with diazophyte and polymicobacterin preparation on the background of straw predecessor, followed by the application of N10 per tonne of by-products, which will ensure a yield of 3.16 t/ha of wheat grain. In the absence of predecessor by-products on the ground, pre-sowing seed treatment with diazophyte and polymicobacterin preparations, as well as the application of N45R45K30, should be used, resulting in a grain yield of 3.50 t/ha.


Please see the link - https://www.ikprress.org/index.php/PCBMB/article/view/5663

RECENT STUDY ON Sclerotinia STEM: A REVIEW| PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 The goal of this article was to compile the literature on Sclerotinia stem rot and to discover trends in the field. Plant Disease is the most active journal in this field, and Field Crops Research has the highest total number of citations per publication. The Journal of Applied Microbiology has the highest total number of citations per publication. India, China, and Australia are the most active and productive countries, with the most articles, citations, and co-authorship links, respectively. The Netherlands has the highest total number of citations per publication. With the most publications and citations, China's Main Laboratory of Integrated Management of Crop Diseases and Pests is the most involved research organisation. The most active organisation is Banaras Hindu University's Department of Botany. The Rajasthan Agriculture University's Agriculture Research Station has the most co-authorship connections. Barbetti M.J. is the most prolific blogger, with the most citations and co-authorship links. The author with the most publications is M.R. Mcdonald With the highest average citation per publication, Bahadur Singh H and Kumar Sarma B are the most active authors.


Please see the link - https://www.ikprress.org/index.php/PCBMB/article/view/5662

EFFECT OF PRE EMERGENCE HERBICIDES AND THEIR COMBINATIONS ON WEEDS INFESTATION, YIELD COMPONENTS AND YIELD OF COMMON BEAN (Phaseolus vulgaris L.) AT GURAFERDA AND MENITSHASHAWOREDA, SOUTH WEST ETHIOPIA | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Weeds reduce the yield and quality of the common bean. During the 2019 main cropping season, the experiment was carried out at Guraferda and Menit Shasha Woreda in Southern Ethiopia (July to October). The goals were to see how pre-emergence herbicides and their combinations affected weed infestation, yield part, and yield of common bean, to see whether low-dose herbicides could be supplemented with hand weeding for effective weed control, and to evaluate the economic viability of various weed management practises. The experiment included 16 treatments: s-metholachlor (1.0,1.5, and 2.0 kg ha-1); pendimethalin (1.0,1.3,1.6 kg ha-1); s-metholachlor plus pendimethalin (1.0,1.3,1.6 kg ha-1); s-metholachlor plus pendimethalin (1.0,1.3,1.6 kg ha-1); s-metholachlor plus pendimethalin (1.0,1.3,1.6 kg ha-1); s-methol 1.0 kg ha-1+ 0.75 kg ha-1 pendimethalin, s-metholachlor at 0.75 kg ha-1+ pendimethalin at 1.0 kg ha-1, s-metholachlor at 0.75kg ha-1+pendimethalin at 1.25 kg ha-1, s-metholachlor at 1.0kg ha-1+one hand weeding at 5WAE, pendimethalin at 1.0 kg ha-1+ one hand weeding at 3WA At crop harvest, the combined use of one hand weeding at 5WAE with 1.0kg ha-1 of s-metholachlor and pendimethalin and two hand weeding at 2and 5 WAE showed lower weed dry biomass at both sites. The weed-free at Guraferda and MenitShasha yielded the highest grain yields (3715.7kg ha-1) and 3437.1kg ha-1, respectively. Grain yield losses were highest (79.48 percent and 67.64 percent, respectively) in weedy check plots at MenitShasha and Guraferda. The application of s-metholachlor at 1.0 kg ha-1 combined with one hand weeding at 5WAE yielded the highest net gain (Birr 27640.1 ha-1).


Please see the link : https://www.ikprress.org/index.php/PCBMB/article/view/5661




EFFECTS OF HEALTH EDUCATION ON PARENTS’ KNOWLEDGE AND ATTITUDES REGARDING VACCINATION AT MOUANKO, EDEA HEALTH DISTRICT, CAMEROON | Journal of Basic and Applied Research International

 Background: Vaccination is estimated to save 2.5 million lives each year around the world. However, due to a lack of vaccination awareness, vaccination coverage in Sub-Saharan Africa, like Cameroon, remains low.

The aim of this study was to see how health education affected parents' awareness and attitudes about vaccination.
The effects of health education on parents' awareness and attitudes about vaccination in the Mouanko Health Area were studied in this cross-sectional interventional analysis. Cluster sampling was used to select participants, who were then randomly assigned to either an experiment or a control group. After performing a pretest, the intervention consisted of health education on vaccination that was only provided to the intervention party. A semi-structured questionnaire was used to collect data on skills and attitudes. A point was awarded for each correct answer to the questionnaire's questions. Parents who scored seven points or more on the information segment were considered intelligent, whereas those who scored less than seven were considered unknowledgeable. Similarly, each correct answer was given a point; parents who scored four points or more on a scale of seven were said to have positive attitudes, while those who scored less than four were said to have negative attitudes.  vaccination viewpoints Data was obtained from May 13 to June 15, 2019, and SPSS version 25.0 was used to analyse it.
Results: After the intervention, the proportion of participants with vaccine awareness increased from 35.0 percent to 57.6 percent, and the proportion of participants with positive attitudes increased from 31.0 percent to 41.9 percent. For information and attitudes, the p-values were 0.001 and 0.012, respectively (CI = 95 percent).

Conclusion: The cost-effectiveness of health education was shown by the positive improvement in the intervention community after the intervention.

Please see the link - https://www.ikprress.org/index.php/JOBARI/article/view/5660

THE EFFECT OF SMOKE WATER EXTRACT ON ENDOGENOUS PHYTOHORMONES OF Cucumis sativus L. SEEDS EXPOSED TO SALT STRESS | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Different concentrations of smoke water extract (0, 1:10, 1:100, 1:200) were used in this study to mitigate the toxic effect of different concentrations of Sodium Chloride (NaCl) (0, 100, 150, 200) mM on Cucumber sativus L. seed germination. The seeds were soaked in two different concentrations of smoke water extract for two different periods of time (24 and 72 hours). Auxin (IAA), gibberelline (GA), cytokinine (CK), and abcsisic acid (AB) concentrations, as well as germination percentage, were all measured. The findings revealed that, depending on the soaking time, smoke water extract has a positive effect on seed germination.

Please see the link - https://ikprress.org/index.php/PCBMB/article/view/5658


Monday, April 19, 2021

PREVALENCE AND MOLECULAR IDENTIFICATION OF Candida spp. ISOLATED FROM VULVOVAGINITIS IN BAGHDAD, IRAQ | Journal of Disease and Global Health

 Candida species-induced vulvovaginitis is a widespread fungal infection among adult and pregnant women. Vulvovaginal candidiasis is caused by Candida albicans (VVC). The participants in the study were 250 women ranging in age from 17 to 45 years old who were attending specialist hospitals in Baghdad, Iraq. Candida spp. was detected using standard methods and biochemical tests after vaginal smears were cultured on selective chrome agar and Sabouraud dextrose agar. Polymerase Chain Reaction (PCR) was also used to identify isolates, with the universal ITS4 area primer and species-specific primers. There were 141 (56.4%) yeast isolates from the 250 vaginal smears obtained, with 81 (57.4%) Candida albicans and 60 (42.6%) non-albicans. Candida albicans was the most common Candida species (57.4 percent), followed by C. glabrata (20.6 percent), C. tropicalis (13.5 percent), and C. krusei (12.5%). Candida spp. were identified by PCR, which verified the primary identification by biochemical tests. The internal transcript spacer (ITS) 1 and 2 primers produced amplification products of 402 bp in the PCR assays for C. albicans in the examined samples and a high sensitivity to molecular detection for Candida species other than albicans among the local isolates. Finally, the high prevalence of Candida isolates among Iraqi female Vulvovaginitis patients highlights the importance of frequent candidiasis screening and evaluation in Iraqi hospitals.


Please see the link :-
https://www.ikprress.org/index.php/JODAGH/article/view/5655

MICROBIAL BIO-PESTICIDES AND BOTANICALS AS AN ALTERNATIVE TO SYNTHETIC PESTICIDES IN THE SUSTAINABLE AGRICULTURAL PRODUCTION | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 While the green revolution has had a significant impact on crop yields, it has failed to integrate with agricultural sustainability. Unnecessary use of chemical fertilisers and pesticides has had negative consequences for human health and the environment. Excessive pesticide use in agroecosystems has irreversible consequences. Microbial bio-pesticides and botanicals are gaining traction as a viable alternative to conventional pesticides. These are not harmful to the climate. In agriculture, bio-pesticides can be used to combat some of the most damaging plant diseases and insect pests. This review article focuses on various types of Microbial Biopesticides (Entomopathogenic Fungi, Bacteria, Virus, and Nematodes) as well as Botanicals or Botanical Pesticides (Neem, Rotenone, Pyrethrum, Ryania, Sabadilla, and Eucalyptus oil) that can be used as an alternative to chemical or synthetic pesticides as a step toward agricultural sustainability. When used correctly, biopesticides have a huge potential to improve agriculture's productivity and environmental protection.


Please see the link :-
https://www.ikprress.org/index.php/PCBMB/article/view/5640

In vitro ANTIFUNGAL EVALUATION OF PREPARED CARBENDAZIM NANOEMULSION FOR EFFECTIVE TRANSLOCATION IN FUNGAL CELL | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 The aim of this study was to see how effective permeability of antifungal carbendazim could be increased by forming it as an o/w nanoemulsion (NE). The NE was created by using an unprompted emulsification technique to optimise the concentrations of oleic acid as the oil, Tween 20 as the surfactant, and acetone as the co-surfactant. TEM (transmission electron microscopic) analysis was used to determine clarity, drug quality, compatibility, pH, viscosity, stability, and scale. In vitro antifungal assay were performed against two phytopathogens, Alternaria alternata and Dreschlera oryzae. The transparent Nano emulsion with narrow size range of oil droplets, sufficient pH and viscosity was obtained. The creation of distinct nanosized droplets with sizes ranging from 80 to 140 nm was revealed by TEM research. In contrast to the marketed Wettable Powder or suspension of Carbendazim, the NEs were stable under variable temperature conditions and had increased permeation into fungal spore cells, resulting in better performance.


Please see the link :-
https://www.ikprress.org/index.php/PCBMB/article/view/5639

WATER-RESOURCE-SAVING TECHNOLOGIES FOR CROP CARE IN SANDY SOILS | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Effective use of land resources, especially desert sands and sandy soils, is becoming an increasingly important and urgent challenge in the consistent solution of the world's population's food supply crisis, which is becoming increasingly globalised due to its high growth rate (up to 11 billion by the end of the century).

After all, developing water-saving agricultural technologies for determining and caring for low-water, fertile, and core crops that are ideal for these soil and climatic conditions is of critical scientific and practical importance.

With the aid of field and laboratory tests, we researched the technologies of sowing, fertilising, and irrigating cotton, sunflower, soybean, peanuts, beans, potatoes, and carrots in order to solve this urgent issue.

Results: Sunflower cultivation in desert sandy soils used 4,362 m3/ha (1,144 m3/ha) less water than typical crops (26.2 percent ).

Specifically, during the period before sunflower harvesting, with intensive soy sunflower sowing and joint care for sunflower and grain lasting 2-3 days, the total amount of irrigating decreased by one time, and water savings of an average of 730 m3/s hectares were achieved.

At the same time, a total of 4395 m3 / ha of water was used for the two crops, and by using ditch water in a 1:1 ratio, 2177 m3 / ha (roughly 50%) of river water could be saved without causing soil and crop harm.

In terms of the length of the growth period of soybean sowing, when sown in pure form, a total of 6 times irrigating is permitted, resulting in 4332 m3/ha of water being expended, while 2577 m3/ha of river water was saved in the ratio of 1:1 without harmful consequences.

Soybean growers were able to plant carrots and grow two crops, using an additional 731 m3/ha of water to do so.

Although caring for legumes up to 11 times, subject to permissible irrigation - up to 8145 m3/ha and water consumption - up to 1606 m3/ha, the overall amount of irrigations decreased by half, seasonal water consumption - up to 1606 m3/ha, or 19.7%, while caring for peanuts up to 11 times, subject to permissible irrigation - up to 8145 m3/ha and water consumption - up to 1606 m3/ha.

In addition, due to the efficient use of water, the drainage ditch was able to conserve 3256 m3/ha of river water in a 1:1 ratio.

22.9-387 percent of the cost of crop care was saved thanks to these innovations.

Conclusion: The study found that yields are very poor, extremely unsuitable for agriculture, decreasing mineralization in desert sandy soils, leading to high water-resource-saving and economic productivity by jointly caring for sunflower and soybean crops, as well as eliminating water shortages.

Please see the link :-
https://www.ikprress.org/index.php/PCBMB/article/view/5638

COMPARATIVE ANALYSIS OF ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF Thuja occidentalis AND Phyllanthus emblica | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Since ornamental plants have antimicrobial and antioxidant properties, they have been widely used as a therapeutic source to treat a wide range of diseases. Owing to the existence of phytocompounds such as Tannins, Flavanoids, Saponin, and others, Thuja occidentalis and Phyllanthus emblica have the ability to treat diseases such as cancer, respiratory diseases, and some infections. The antioxidant potential of both the plants collected from the Chandigarh University botanical garden was calculated by measuring the IC50 value of plant extracts prepared in water with varying concentrations of each plant extract, as well as their synergistic antioxidant potential using the DPPH assay for free radical scavenging. The antimicrobial ability of both the plants was observed using the Agar well diffusion method and the Microtitre plate dilution method to assess the effective dosage of both the plants by measuring the IC50 for both the plants as well as their blend in the antimicrobial assay. Both plants had antioxidant and antimicrobial properties, but Phyllanthus emblica had a higher therapeutic ability than Thuja occidentalis. Thus, the synergistic effect of both plants was observed, which has not been recorded previously, and exhibited more effective potential than the plants alone, and can be a better option for ailment of certain diseases and can be utilised as anti ageing and anti cancerous drugs than synthetically obtained drugs, as well as a source of recovering from diseases that can be Gastrointestinal such as diarrhoea, a better option for ailment of certain diseases and can be utilised as anti ageing and anti cancerous drugs than As a result, Phyllanthus and Thuja have the potential to be a better potential treatment for the relief and cure of such diseases.


Please see the link :-
https://www.ikprress.org/index.php/PCBMB/article/view/5637