GREEN SYNTHESIS, ANTI INFLAMMATORY AND ANTIOXIDANT PROPERTY OF Cardiospermum halicacabum MEDIATED COPPER NANOPARTICLE | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Introduction: The implications of biosynthesis of nanomaterials to be incorporated with naturally accessible substances are being evaluated in recent developments in nano biomedicine, an evolving area of medicine. Green synthesis of Copper nanoparticles using plant compounds appears to be a promising method for drug production. This field of research has proved to be very cost effective and satisfactory. The anti-inflammatory and antioxidant properties of Cardiospermum halicacabum induced copper nanoparticles will be evaluated in this review.

The aim of this analysis was to determine the anti-inflammatory and antioxidant properties of copper nanoparticles mediated by C.halicacabum.

Materials and Methods: The approach involves a green synthesis of copper nanoparticles mediated by C.halicacabum, accompanied by anti-inflammatory and antioxidant activity studies.

Results: The anti-inflammatory and anti-oxidant activity of the copper nanoparticle mediated by C.halicacabum increased proportionately with increasing dose concentration.

Conclusion: Copper nanoparticles' wide applicability in a variety of fields, including medicinal, mechanical, and electronic, has fueled research into copper nanoparticles that provide a more direct path of action and are more cost effective. As a result of their cost effectiveness and lack of toxicity, green methodologies have gained more interest and excitement so far. As a result, the properties have changed. Copper nanoparticles mediated by C. halicacabum showed important anti-inflammatory and anti-oxidation activity, making them potent anti-inflammatory and antioxidant agents.

Please see the link:- https://www.ikprress.org/index.php/PCBMB/article/view/5604

SYNERGISTIC ANTI INFLAMMATORY AND ANTIOXIDANT ACTIVITY OF CHLOROGENIC ACID AND L – THEANINE | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Objective: To examine the synergistic influence of L theanine and chlorogenic acid on antioxidant and anti-inflammatory function.

Introduction: Chlorogenic acid ,an ester of caffeic acid and quinic acid is a major phenolic compound in coffee ,daily intake in coffee drinkers is 0.5-1g .Chlorogenic acid and caffeic acid are antioxidants in vitro and might therefore contribute to the prevention of cardiovascular disease. The antioxidant activities are due to phenolic compounds which are important in biological systems because of their production of ROS which may be related to degenerative disease processes such as DNA damage, lipid peroxidation etc. L- theanine is a water soluble amino acid isolated from green tea. Camellia sinensis teas have attracted a great deal of attention due to their numerous health benefits, including antioxidant, hypoglycaemic, anti carcinogenic, antimicrobial, antiinflammatory activities.

Materials and Methods: Chlorogenic Acid and L-Theanine filtered compounds were used. DPPH radical scavenging was used to determine the antioxidant activity and the albumin denaturation inhibition assay was used to determine the anti inflammatory activity.

Results and Discussion: In a concentration based manner, the compound showed both antioxidant and anti inflammatory activity. The synergistic effect thus exhibits antioxidant activity and anti inflammatory activity.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5337

ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF Phyllanthus niruri MEDIATED SILVER NANOPARTICLES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Aim: The aim of study is to evaluate the characterization and anti-fungal property of Phyllanthus niruri mediated silver nanoparticle

Introduction: Phyllanthus niruri is a tropical plant discovered in coastal areas also called as gale of the wind, stonebreaker or seed-under-leaf. The plant is of therapeutic significance for various illness like, influenza, vaginal inflammation, cancer, diabetes mellitus, diuretics, icterus, nephrolithiasis, indigestion. Green synthesis of nanoparticles denotes an improvement over other techniques  because it is manageable, profitable, and fairly reproducible, and often results in more stable materials.Nanotechnology produced substances of various types at nanoscale level. ZnO NPs can proceed as smart weapons against multiple drug-resistant microorganisms and as an alternative for antibiotics.

Materials and Methods: Preparation of Phyllanthus niruri mediated silver nanoparticles was followed by antioxidant activity evaluated by DPPH activity and antimicrobial activity. 

Results: The study shows that Phyllanthus niruri have great potential as a natural antioxidant and antimicrobial source. DPPH radical-scavenging activity of all the extracts of Ph. niruri revealed very high potency. The standard solution used showed 85% of inhibition.

Conclusion: The antibacterial and antifungal effect of Phyllanthus niruri mediated AgNP which were evaluated against the pathogen lactobacillus, S. mutans, C. albicans showed a significant activity. Anti oxidant activity by inhibition of the DPPH radical in vitro showed that the Phyllanthus niruri AgNP was equally potent compared to Standard Ascorbic acid. DPPH radical-scavenging activity of all the extracts of Ph. niruri revealed very high potency. The standard solution used showed 85% of inhibition to the acid radical.ZnO NPs can proceed as smart weapons against multiple drug-resistant microorganisms and as an alternative for antibiotics.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5335
    

GREEN SYNTHESIS OF L-THEANINE MEDIATED SILVER NANOPARTICLES AND THEIR ANTI INFLAMMATORY AND ANTI OXIDANT PROPERTY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Introduction: In recent years, the development of potent green chemistry techniques for making metal nanoparticles has become a prime focus of researchers. They have delved in order to discover an eco-friendly method for production of well-characterized nanoparticles. One of the most advised techniques is production of metal nanoparticles with the help of organisms. Among these organisms plants seem to be the best entrants and they are suitable for large-scale biosynthesis of nanoparticles. Nanoparticles produced by plants are very much stable and the level of synthesis is quicker when compared to microorganisms.

Methodology: In Anti inflammatory, 2 mL of 1% Bovine Albumin fraction was mixed with 400 µL of L-theanine mediated Ag NP in different concentrations (10-50 µg/ml). In Antioxidant, 2 mL of L-theanine mediated Ag NP in the concentration range of 10-50-µg/mL was prepared in 50% methanol solution and added to DPPH solution.

Results and Discussion: When compared with the standard solution, the nanoparticle solution used is very close to being apt as the anti oxidant and the anti inflammatory and only has a slight variation. And the variation being very slight, it can be introduced into the market as a natural product unlike the standard which is a synthetic product.

Conclusion: L-theanine mediated silver nanoparticles are a good anti-inflammatory and anti-oxidant substance. Its activity increases with increase in dosage.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5332

ANTIOXIDANT ACTIVITY OF ZINC OXIDE NANOPARTICLES PREPARED USING CORIANDER OLEORESIN | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Aim and Background: Coriander oleoresin is a natural food additive used to mask the unpleasant taste of various medicines in the pharmaceutical industry. Zinc oxide nanoparticles (ZnONPs) have a wide range of applications and can be prepared from various herbal extracts. In this study, coriander oleoresin mediated ZnONPs were prepared and evaluated for its antioxidant activity.

Materials and Methods: Zinc oxide nanoparticles were synthesized using coriander oleoresin, zinc sulphate and distilled water and kept in an orbital shaker to ensure constant mixing. The formation of nanoparticles was confirmed both visually and spectrophotometrically. The coriander oleoresin mediated ZnONPs were evaluated for its antioxidant activity using DPPH assay and was compared with the standard, ascorbic acid.

Results and Discussion: The peak of nanoparticle formation was observed at 650 nm. The DPPH assay revealed a dose-dependent antioxidant activity of Coriander oleoresin mediated ZnONPs. The highest percentage inhibition obtained was 87.9% with 50 μL, the maximum concentration of nanoparticle used for the study compared with the standard 92.2%.

Conclusion: The present study revealed that ZnONPs prepared using Coriander oleoresin exhibited good antioxidant activity in comparison with ascorbic acid. The antioxidant property of Coriander oleoresin mediated zinc oxide nanoparticles may be used for a wide range of applications where antioxidant is required after further validation.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5329