In vitro ANTI-INFLAMMATORY ACTIVITY USING FRUIT FORMULATION MEDIATED ZINC OXIDE NANOPARTICLES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 The aim of this study was to see whether zinc oxide nanoparticles made from Punica granatum and Elettaria cardamomum had anti-inflammatory properties.

Background: Non-steroidal anti-inflammatory drugs (NSAIDs) such as aspirin, diclofenac, and ibuprofen are the most widely prescribed and used drugs for inflammation and its related conditions. Unfortunately, these medications have a lot of negative side effects. As a result, there has been an increase in the quest for a more powerful and secure alternative. To minimise the side effects, zinc oxide nanoparticles, which have strong anti-inflammatory efficacy, were prepared using a green method mediated by extracts from Punica granatum and Elettaria cardamomum.

Materials and Methods: Punica granatum and Elettaria cardamomum extracts were collected and prepared, zinc oxide nanoparticles were synthesised, nanoparticles were collected using centrifugation, nanoparticles were characterised, anti-inflammatory activity of ZnO nanoparticles was determined using ultraviolet-visible spectroscopy, and albumin denaturation inhibition was tested.

Results: Zinc oxide nanoparticles induced by Punica granatum and Elettaria cardamomum demonstrated anti-inflammatory activity comparable to Diclofenac.

Conclusion: The findings of the study indicate that zinc oxide nanoparticles induced by Punica granatum and Elettaria cardamomum have potent anti-inflammatory properties, and more studies can be done to test it for various medicinal purposes.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5628

ANTI-INFLAMMATORY ACTIVITY OF Maranta arundinacea MEDIATED ZINC OXIDE NANOPARTICLES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Background: In today's world, synthesis of nanoparticles using plants such as arrow roots is considered an environmentally friendly technology because no toxins or chemicals are used. Zinc is a naturally occurring factor that helps macromolecules preserve their structure and function. It aids enzymes that aid in the regulation of cellular processes and pathways. Arrow root-derived zinc oxide nanoparticles have a wide range of biomedical applications. A typical green synthesis technique is used to make these zinc oxide nanoparticles. Zinc nanoparticles have an impact on cell viability, antioxidant function, and the degradation of contaminants in the atmosphere, as previously stated. It is also said to be a safe type of nanoparticle that functions as a natural human skin barrier, according to other reports. Maranta arundinacea, also known as arrowroot, is a perennial plant with a wide range of medicinal properties. It's used to treat diarrhoea, vomiting, indigestion, and even urinary tract infections, as well as being a baby food.

The aim of this study was to look at the anti-inflammatory properties of zinc oxide nanoparticles mediated by Maranta arundinacea.

Materials and Methods: Arrowroot was used to make zinc oxide nanoparticles, which were then tested for anti-inflammatory activity.

The graph obtained shows that arrowroot muted Zinc oxidants have major antiinflammatory activity.

Conclusion: Since Arrow root has a strong anti-inflammatory effect, it can be used as a better drug of choice.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5625

ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF ZINC OXIDE NANOPARTICLES SYNTHESIZED USING Maranta arundinacea AGAINST ORAL PATHOGENS | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Introduction: Nanoparticles are a special category of materials with particular characteristics and in many fields have broad applications. Arrowroot is a starch that is derived from many tropical plant rhizomes, usually Maranta arundinacea, and also contains strong medicinal and antimicrobial properties. Hence, Maranta arundinacea synthesis ZnO nanoparticles are the field of focus here.

Materials and Methods: Arrow root extract preparation: Fresh arrowroot powder has been obtained from the market. One gramme of arrow root powder extract that has been freshly collected is mixed with 100 ml of water and boiled for three to five minutes. Using filter paper, this mixture is filtered.

Preparation of Zinc Oxide Nanoparticles: In 50ml of distilled water, 0.861g of Zinc Sulphate is applied. An extract of this arrow root has been added. The solution was combined with plant extract and the ZnO nanoparticles were prepared using it.

Evaluation of antimicrobial activity: antibacterial and antimicrobial activity The green synthesis of ZnO nanoparticles has been evaluated against oral pathogens S.mutans, Lactobacillus, C. Albicans. Albicans.

Observation: The inhibition zone for zinc oxide nanoparticles was observed and it was calculated that the antifungal activity of the zinc oxide nanoparticles against Candida albicans was large. The nanoparticles of zinc oxide have also been found to provide greater antibacterial activity than the typical drug used.

Results: As a consequence, it is clear that nanoparticles of zinc oxide had greater antimicrobial activity and this is preferred for the next generation to be used as the drug of preference.

Goal: The aim of this study was to determine the antibacterial and antifungal activity of Maranta arundinacea-synthesized zinc oxide nanoparticles.


Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5372

CYTOTOXIC EFFECT OF GRAPE SEED MEDIATED ZINC OXIDE NANOPARTICLES USING BRINE SHRIMP LETHALITY ASSAY - In vitro STUDY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Background: Zinc oxide nanoparticles are a type of metal oxide nanoparticles with a band gap of 3.3 eV and excitation binding energy of 60 meV at room temperature. It has gained considerable attention due to its unique catalytic, antibacterial, antifungal, photochemical, UV-filtering, anti-inflammatory properties owing to its large surface area to volume ratio. Synthesis of metal and metal oxide nanoparticles through green routes and environmentally benign methods has been focused by researchers to avoid the use of toxic chemicals making the process and the final product eco-friendly and non-toxic in nature.

Aim: To assess the cytotoxic effect of grape seed mediated zinc oxide nanoparticles using brine shrimp lethality assay.

Materials and Methods: Plant extracts were collected and prepared for obtaining the grape seed extract. Zinc oxide nanoparticles were then synthesised in a methodological manner, followed by the assessment and characterisation of the zinc oxide nanoparticles. The zinc oxide nanoparticles powder was then prepared. Brine shrimp lethality assay was the test used to identify the cytotoxic activity of the grape seed mediated zinc oxide nanoparticles and the results were statistically analysed.

Results: Cytotoxic activity of zinc oxide nanoparticles was verified through several tests such as Brine shrimp assay. It was observed that the initial number of nauplius reduced in a negligible amount after one day and very little after three days in only certain concentrations of Zinc oxide nanoparticles.

Conclusion: The grape seed mediated zinc oxide nanoparticles thus show very less or negligible cytotoxic activity as the concentrations were increased and thus the zinc oxide nanoparticles are expected to be used in future for effective drug systems and in the future drug formulations.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5360

ANTIOXIDANT ACTIVITY OF ZINC OXIDE NANOPARTICLES PREPARED USING CORIANDER OLEORESIN | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Aim and Background: Coriander oleoresin is a natural food additive used to mask the unpleasant taste of various medicines in the pharmaceutical industry. Zinc oxide nanoparticles (ZnONPs) have a wide range of applications and can be prepared from various herbal extracts. In this study, coriander oleoresin mediated ZnONPs were prepared and evaluated for its antioxidant activity.

Materials and Methods: Zinc oxide nanoparticles were synthesized using coriander oleoresin, zinc sulphate and distilled water and kept in an orbital shaker to ensure constant mixing. The formation of nanoparticles was confirmed both visually and spectrophotometrically. The coriander oleoresin mediated ZnONPs were evaluated for its antioxidant activity using DPPH assay and was compared with the standard, ascorbic acid.

Results and Discussion: The peak of nanoparticle formation was observed at 650 nm. The DPPH assay revealed a dose-dependent antioxidant activity of Coriander oleoresin mediated ZnONPs. The highest percentage inhibition obtained was 87.9% with 50 μL, the maximum concentration of nanoparticle used for the study compared with the standard 92.2%.

Conclusion: The present study revealed that ZnONPs prepared using Coriander oleoresin exhibited good antioxidant activity in comparison with ascorbic acid. The antioxidant property of Coriander oleoresin mediated zinc oxide nanoparticles may be used for a wide range of applications where antioxidant is required after further validation.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5329