ANTI- INFLAMMATORY AND ANTI- MICROBIAL ACTIVITY OF Hybanthus enneaspermus MEDIATED SILVER NANOPARTICLES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 The first paragraph is an introduction. Nanotechnology is an area of research and development that focuses on making 'things' on the scale of atoms and molecules, such as materials and devices. Green nanoparticle synthesis prevents the development and use of hazardous compounds, ensuring non-toxicity. The esterification of cinnamic acids produces chlorogenic acids, which are phenolic compounds. They have a wide variety of pharmacological properties. Our research focuses on the green synthesis of silver nanoparticles mediated by Hybanthus enneaspermus and the evaluation of their antimicrobial and anti-inflammatory properties. In nanoscience, nanotechnology, and nanomedicine, silver nanoparticles (AgNP) play a significant role. Antibacterial, antifungal, antiviral, anti-inflammatory, anti-antigenic, and anti-cancer properties have been discovered in AgNP.

The aim of this study is to evaluate the anti-inflammatory and anti-microbial properties of silver nanoparticles mediated by Hybanthus enneaspermus.

Materials and Methods: The technique involves a green synthesis of Silver nano particles mediated by Hybanthus enneaspermus, accompanied by antimicrobial and anti-inflammatory studies. The diffusion approach will be used to investigate the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in antimicrobial activity. Diclofenac sodium is used as a standard for anti-inflammatory activity in various concentrations. The experiment was carried out three times.

Results: Silver nanoparticles mediated by Hybanthus enneaspermus showed a proportionate increase in antimicrobial and antioxidation activity with growing dose concentration.

Conclusion: Silver nanoparticles mediated by Hybanthus enneaspermus demonstrated strong antimicrobial and antioxidation activity, and they are considered to be excellent antimicrobial and anti-inflammatory agents.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5614

CYTOTOXIC EFFECT AND ANTIOXIDANT ACTIVITY OF SILVER NANOPARTICLES SYNTHESISED USING HERBAL FORMULATION OF Ocimum sanctum AND Justicia adhatoda | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Background: Nanotechnology is a relatively new field of pharmacological research that has a wide range of applications in the biomedical, engineering, chemical, pharmaceutical, and food industries. The use of microorganisms and herbal extracts for nanoparticle synthesis has attracted international attention, and it is one of the most interesting nanotechnologies. Because of their versatility, silver nanoparticles have a lot of potential in biomedical research. Silver has been found in many bacteria and microorganisms, and it has been discovered that it inhibits their growth and existence. In plant biotechnology, green synthetic techniques for the development of nanoparticles have recently sparked a lot of interest. Biologically synthesised silver nanoparticles are more beneficial than chemical reductions because they are eco-friendly, low-cost, and one-stage processes. Tulsi, chitharathai, adathoda, turmeric, and other herbs found in the Indian subcontinent have various medicinal values and have been used in traditional medicines and home remedies, such as tulsi, chitharathai, adathoda, and turmeric. Justicia adhatoda (adathoda) contains photochemical compounds, and Ocimum sanctum (tulsi) contains germicide and disinfectant. Ocimum Sanctum (Tulsi) and Justicia adhatoda (adathoda), according to earlier research, have a wide range of medicinal properties, including antifungal, antiviral, antibacterial, and cytotoxic properties.

The aim of this research is to use UV Visible Spectroscopy to investigate the antioxidant and cytotoxic activity of silver nanoparticles mediated by herbal extracts of Ocimum sanctum and Justicia adhatoda.

Materials and Methods: Ocimum sanctum and Justicia adhatoda herbal extracts were prepared in our laboratory from a preexisting powder to achieve green synthesis of silver nanoparticles. The physical characterization of these silver nanoparticles, such as size and shape, was confirmed using UV-visible spectroscopy, and the cytotoxic and antioxidant activity was tested and characterised using UV-visible spectroscopy.

At 450 nm, the surface plasmon resonance band (SPR) was measured. The antioxidant activity of nanoparticles increased as concentration was increased, and cytotoxic activity was measured using the number of live shrimps, which decreased as concentration was increased. The percentage inhibition of antioxidant activity was 50 mu/L, and the percentage inhibition of cytotoxic activity was 25 mu/L.

Conclusion: Silver nanoparticles made from Ocimum Sanctum (Tulsi) and Justicia adhatoda (adathoda) were biosynthesised in a novel way and showed significant antioxidant and cytotoxic activity with minimal side effects. This is the first report of Tulsi and adhatoda-mediated silver nanoparticle synthesis with such properties, which will need to be investigated further using other characterization and testing methods.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5566

ANTIBACTERIAL ACTIVITY AND CYTOTOXICITY OF AMLA SEED MEDIATED GRAPHENE OXIDE, SILVER NANOPARTICLE & Go-Ag NANOPARTICLE - AN in vitro STUDY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 The study's goal was to make Amla seed-mediated silver nanoparticles, graphene oxide nanoparticles, and GO-Ag nanocomposite and test their antibacterial activity against Streptococcus mutans, Lactobacillus, and Candida albicans. The cytotoxicity of amla seed mediated graphene oxide nanoparticles and silver nanoparticles, as well as GO-Ag nanocomposite, is then determined using the Brine shrimp lethality assay.

Amla seed extract was prepared using the following materials and methods. Silver, graphene oxide nanoparticles, and GO-Ag nanocomposites were biosynthesised using the filtrate. The samples were measured for maximum absorbance using UV-Visible spectrophotometry after 24 hours of incubation. To produce the synthesised silver nanoparticles, graphene oxide nanoparticles, and GO-Ag nanocomposite, all of the samples were heat dried. Various concentrations of biosynthesized Amla seed mediated silver nanoparticles, graphene oxide nanoparticles, and GO-Ag nanocomposite (50,100,150 g/ml) were tested for antibacterial activity against Streptococcus mutans, Lactobacillus, and Candida albicans. The cytotoxicity of different nanoparticle concentrations was determined using the Brine Shrimp Lethality Assay.

The colour of AgNPs changed from colourless to reddish brown, the colour of GO NPs changed from brown to black, and the colour of GO-Ag nanocomposite changed from black to a darker intensity of black. The formation of their respective nanoparticles is confirmed by the colour change. Antibacterial activity of silver nanoparticles against Streptococcus mutans.

Lactobacillus (150 ug/ml – 17 mm zone of inhibition) and Lactobacillus (150 ug/ml – 20 mm zone of inhibition) all showed 20% lethality at a concentration of 25 ul. Graphene oxide nanoparticles have strong antibacterial activity against Lactobacillus (150 ug/ml – 20 mm zone of inhibition) and demonstrated 40% lethality at a concentration of 25 ul. The antibacterial activity of the GO-Ag nanocomposite was negligible against all of the test organisms, with 10% lethality at 20 and 25 ul. Antibacterial activity against Candida albicans was limited in all three nanoparticles (9 mm Zone of Inhibition).

Conclusion: Amla fruit seed extracts were used to make silver nanoparticles, graphene oxide nanoparticles, and GO-Ag nanocomposites. Silver, graphene oxide nanoparticles, and the GO-Ag nanocomposite all had a strong antibacterial impact against oral pathogens while causing minimal cytotoxicity.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5555

CHARACTERIZATION OF Asparagus racemosus MEDIATED SILVER NANOPARTICLES USING UV - VIS SPECTROPHOTOMETER AND SEM | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Nanoparticles have attracted a lot of interest and have had a major influence on material science. Because of its technological significance, the dominance of nanoparticles will continue in future years with the implementation of incessant research in this sector. The object of the research was to characterize the A. Using a UV Vis spectrometer, racemosus mediates silver nanoparticles. The source of A. Racemosus was obtained as an extract and prepared. For the reduction of Ag+ ions to silver nanoparticles, this extract was used. For the synthesis of nanoparticles, 1 mM of 90 mL silver nitrate solution was prepared and 10 mL of 100 mg plant extract was added and stored in a shaker. The UV visible spectrometer and the scanning electron microscope were used to examine and classify nanoparticles. The Peak appears to confirm the synthesis of nanoparticles using a UV-vis spectrophotometer at 420 nm and the mean size of the nanoparticles has been found to be 150 nm. When used at sufficient concentrations, silver nanoparticles act as a safe alternative to other antimicrobial agents that are chemically derived. Silver nanoparticles may, therefore, be a better alternative agent for the cleaning and disinfection of food industry equipment and surfaces.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5538

GREEN SYNTHESIS OF ZINC OXIDE NANOPARTICLES USING GRAPE SEED EXTRACT AND ITS CHARACTERIZATION | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Background: Nanotechnology (NPs) is a modern science technology with major applications, including industries in medicine and pharmacology. In this research, the processing of zinc oxide nanoparticles from non-toxic grape seed extract as a reducing agent has been used for cost-effective and eco-friendly green synthesis.

Objective: To synthesise and characterise zinc oxide nanoparticles mediated by grape seed extract.

Materials and Methods: Green synthesis was used to synthesise the zinc oxide nanoparticles. Morphological features such as the form and size of the green synthesised zinc nanoparticles obtained via transmission electron microscopy (TEM).

Results: The results indicate that the nanoparticles mediated by the synthesised grape seed extract are eco-friendly, good and non-toxic. In the graph seed extract solution, the visual colour change observed indicates the presence of nanoparticles of zinc oxide. The particle size ranges from 20 to 30 microns, clearly demonstrating the existence of ZnO NPs in spherical form. At 410 nm, the absorption produced in the reaction media has an absorbance peak. The TEM exhibits spherical shapes and nanoparticles that are flexible. They seem to be structured in an open superstructure and cluster.

Conclusion: A simple, biological and low-cost approach to the preparation of zinc oxide nanoparticles using grape seed extract was conducted in this report. In order to know the efficacy of these nanoparticles, synthesised grape seed extract mediated zinc oxide nanoparticles can therefore be subjected to various other biological activities such as antibacterial, antifungal, cytotoxic assessment.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5399

ANTIBACTERIAL PROPERTIES OF LODHRA BARK (Symphlocus racemosa) MEDIATED SILVER NANOPARTICLES AGAINST ORAL PATHOGENS | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Aim: The absolute objective of the analysis is to discover the antibacterial properties of silver nanoparticles mediated by Lodhra bark against oral pathogens.

Materials and Methods: The plant extract, which is used to synthesise nanoparticles, is collected and dried and powdered and mixed with water. Using the agar well diffusion process, synthesised nanoparticles were tested for their antimicrobial activity against oral pathogens.

Results and Discussion: Antibacterial and antifungal operation of oral pathogens is carried out from synthesised nanoparticles and the inhibition zone is shown.

Conclusion: Lactobacillus sp and Candida albicans are affected by silver nanoparticles.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5378

ANTIFUNGAL AND ANTIBACTERIAL EFFECTS OF CORIANDER OLEORESIN MEDIATED SILVER NANOPARTICLES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Goal: To test the antibacterial and antifungal activity of silver nanoparticles mediated by coriander oleoresin.

Materials and Methods: Silver nanoparticles mediated by coriander oleoresin were initially prepared and validated using UV-Visible Spectroscopy. The prepared silver nanoparticles mediated by Coriander oleoresin were further evaluated by standard procedure against S for their antimicrobial properties. Lactobacillus, S. Mutans, S. Aureus, C. Albicans.

Results and Discussion: The study demonstrated peak UV-Visible spectroscopy at 420 nm, suggesting synthesis of silver nanoparticles. Dose-dependent antibacterial activity with S was demonstrated. With aureus and S. Muthans, mutans. S. Coriander oleoresin-mediated silver nanoparticles with a maximum inhibition zone of 22 mm at their peak concentration (150μL) were very sensitive to aureus. At all concentrations used, Lactobacillus showed the same zone of inhibition. C. Albicans, at their maximum concentration (150 μL) of nanoparticles, have the highest inhibition zone (20 mm).

Conclusion: This study concludes that silver nanoparticles induced by Coriander oleoresin have strong antimicrobial activity and can be used for infections caused by S. Mutans, S. Mutans Aureus, C. Albicans. Albicans.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5370

ANTIBACTERIAL ACTIVITY OF CINNAMON - CLOVE MEDIATED SILVER NANOPARTICLES AGAINST Streptococcus mutans | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Context: There are protective properties of cinnamon and clove against oral pathogens. Streptococcus mutans is responsible for the creation of cavities. Synthetic drugs were used to the full and the scientific area in terms of medicine was taken over by newer nanoparticles. As they appear to have little or no side effect on human health, herbal extracts contained abundantly need to be used more.

Aim: This research was conducted to test the antibacterial efficacy against Streptococcus mutans of Cinnamon-Clove mediated Silver nanoparticles.

Materials and Methods: We procured herbal powders. After maximum activity was noted by UV spectroscopy, an aqueous extract was prepared and centrifugated. The inoculated culture plates were added to different volumes of the Silver nanoparticles containing Cinnamon and Clove combinations. The inhibition zones were measured using the technique of disc diffusion.

Results: Cinnamon-Clove mediated Silver nanoparticles had the highest amount of good antibacterial activity (10 mm inhibition zone) and this property was greater than that of widely used antibiotics (Amoxicillin-8 mm).

Conclusion: Cinnamon and Clove mediated Silver nanoparticles were examined and good antibacterial efficacy was indicated by their wider inhibition zones and this property was shown to be similar to an antibiotic. This new formulation for use against oral pathogens such as Streptococcus mutans, which is positively correlated with dental caries and periodontal disorders, can be integrated into mouthwashes and probiotic lozenges.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5348

HING OIL MEDIATED SYNTHESIS OF SILVER NANOPARTICLES, CHARACTERISATION AND ITS ANTIMICROBIAL ACTIVITY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Aim: To prepare silver nanoparticles from hing oil and to test their antimicrobial activity was the objective of the research.

Materials and Methods: In a conical flask 0.5 mL of hing oil was mixed with 99.5 mL of distilled water. To this, 0.0169 grams of silver nitrate was added and kept in an orbital shaker for the formation of nanoparticle. The colour change was noted visually and the absorbance was noted using UV -Vis spectroscopy. The final product was centrifuged and NPs were collected. The morphology was studied using TEM. The hing oil mediated silver nanoparticles were evaluated for its antimicrobial activity by agar well diffusion method using standard procedure. The antimicrobial activity was tested against S. aureus, S. mutans and Lactobacillus and Candida albicans.

Results and Discussion: The formation of silver nanoparticles at a peak of 420 nm was confirmed by UV-Vis spectroscopy. Spherical and rod-shaped nanoparticles with a size ranging from 10-55 nm were shown by TEM. The antibacterial and antifungal activity of the silver nanoparticles induced by the hing oil was comparable. With an increase in concentration, there was no difference in the inhibition region.

Conclusion: Silver nanoparticles mediated by Hing Oil may be a safer option for infections caused by bacteria like S. aureus, S. Although the zone of inhibition shown was similar in the different concentrations used in the analysis, mutans and Lactobacillus and fungi such as Candida albicans were similar. However, for their further pharmacological function, hing oil mediated green synthesis of silver nanoparticle was successful and can be further explored. For its other biomedical uses, Hing oil mediated silver nanoparticles can be assessed.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5342

GREEN SYNTHESIS OF L-THEANINE MEDIATED SILVER NANOPARTICLES AND THEIR ANTI INFLAMMATORY AND ANTI OXIDANT PROPERTY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Introduction: In recent years, the development of potent green chemistry techniques for making metal nanoparticles has become a prime focus of researchers. They have delved in order to discover an eco-friendly method for production of well-characterized nanoparticles. One of the most advised techniques is production of metal nanoparticles with the help of organisms. Among these organisms plants seem to be the best entrants and they are suitable for large-scale biosynthesis of nanoparticles. Nanoparticles produced by plants are very much stable and the level of synthesis is quicker when compared to microorganisms.

Methodology: In Anti inflammatory, 2 mL of 1% Bovine Albumin fraction was mixed with 400 µL of L-theanine mediated Ag NP in different concentrations (10-50 µg/ml). In Antioxidant, 2 mL of L-theanine mediated Ag NP in the concentration range of 10-50-µg/mL was prepared in 50% methanol solution and added to DPPH solution.

Results and Discussion: When compared with the standard solution, the nanoparticle solution used is very close to being apt as the anti oxidant and the anti inflammatory and only has a slight variation. And the variation being very slight, it can be introduced into the market as a natural product unlike the standard which is a synthetic product.

Conclusion: L-theanine mediated silver nanoparticles are a good anti-inflammatory and anti-oxidant substance. Its activity increases with increase in dosage.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5332

SYNTHESIS OF SILVER NANOPARTICLES USING Andrographis paniculata AND Phyllanthus niruri FORMULATION AND ITS ORAL PATHOGEN CONTROL | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Aim: To synthesize silver nanoparticles using Andrographis paniculata and Phyllanthus niruri formation and its oral pathogen control.

Introduction: Silver nanoparticles are recognized more and more these days because of its wide range of application in all fields of study. Silver nanoparticles are synthesized using the plants Andrographis paniculata and Phyllanthus niruri which were used for traditional medicine in olden days. In this study we are synthesizing silver nanoparticles using green synthesis and finding its effects against oral pathogens.

Materials and Methods: 1. Collection of Andrographis paniculata and Phyllanthus niruri.

Synthesis of silver nanoparticles using an herbal formulation using Andrographis paniculata and Phyllanthus niruri.

To conclude the oral pathogen growth in in vitro with agar well method of pathogen control test with gram positive round shaped bacterium Staphylococcus aureus, Streptococcus mutans, Pseudomonas species and Enterococcus species.

Results: The silver nanoparticles can be synthesized using herbal formulation using Andrographis paniculata and Phyllanthus niruri. They had a useful action against oral pathogens with less side effects.

Conclusion: The silver nanoparticles were synthesized using herbal formulation of Andrographis paniculata and Phyllanthus niruri. The silver nanoparticles had activity against oral pathogens like Staphylococcus aureus, Streptococcus mutans, Pseudomonas species and Enterococcus species and can be helpful in the health care centre to use against various diseases with least side effects.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5328

In vitro CYTOTOXIC EFFECT OF ZIRCONIUM OXIDE NANOPARTICLE AND ITS ANTIBACTERIAL ACTIVITY AGAINST ORAL PATHOGENS | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Introduction: From last decay nanotechnology is playing advance in various fields in the research field. Nanoparticles are the most required scientific tool that acts as a bridge between atomic or molecular or atomic structure with bulk material. They have unique      properties like magnetic, optical and excellent in catalytic. In this study we are going to see the In vitro cytotoxic effect of zirconium oxide nanoparticle and its antibacterial activity against oral pathogens.

Materials and Methods: Zirconium oxide nanoparticles are synthesized by taking Azadirachta indica and aloe vera plant extract and nanoparticle powder is prepared. And then in vitro cytotoxic effect is done by using brine shrimp lethal assay.And then antibacterial activity test is done against the oral pathogens.

Results: Zirconium oxide nanoparticles are toxic if the concentration is increased but in lower concentrations the function is best. The action against the oral pathogens. In small concentrations S. aureus showed an 8mm zone of inhibition when the concentration increased S. mutans showed 11mm zone of inhibition.

Conclusion: The zirconium oxide nanoparticles can be used as antibacterial agents against the oral pathogens in small quantities. Thus in future nanoparticles are going to role the developing countries in various fields of medicine.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5319

ANTIFUNGAL ACTIVITY OF CINNAMON - CLOVE MEDIATED SILVER NANOPARTICLES AGAINST Candida albicans | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Background: Human infections caused by Candida albicans range from common oral thrush to fatal systemic superinfections in patients who are afflicted with other diseases. The antifungal actions of silver nanoparticles have been broadly investigated in comparison with other metals. Clove and cinnamon are recognised for their antimicrobial properties.

Aim: This study was conducted to assess the antifungal activity of cinnamon-clove mediated silver nanoparticles against Candida albicans.

Materials and Methods: The herbal powders were procured. An aqueous extract was prepared and centrifuged after maximum activity was noted by UV visible spectroscopy. Various concentrations of the silver nanoparticles containing cinnamon and clove combinations were added into the inoculated culture plates. The zones of inhibition were calculated by disk diffusion method.

Results: Cinnamon-clove mediated silver nanoparticles have good antifungal activity (10 mm-zone of inhibition) in their highest concentration.

Conclusion: Cinnamon and clove mediated silver nanoparticles were studied and their wider zones of inhibition were suggestive of good antifungal efficiency but not as good as the control. This new formulation can be incorporated into natural mouthwashes and probiotic lozenges for use against oral pathogens like Candida albicans, which is positively associated with oral thrush.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5318

ANTIOXIDANT AND CYTOTOXIC EFFECTS OF SILVER NANOPARTICLES SYNTHESISED USING HING OIL | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Aim: To find out the antioxidant and cytotoxic effect of Hing oil mediated silver nanoparticles syntheszsed .

Materials and Methods: 0.5mL of hing oil was dissolved in 99.5 mL of distilled water in a conical flask and to this 0.0169gm of silver nitrate was added; the reaction mixture was kept in an orbital shaker with magnetic stirrer and  the colour change was noted. Every hour the absorbance was noted using UV-Visible spectroscopy and the final product was centrifuged for 10 minutes and nanoparticles were collected. DPPH assay was carried out with standard procedure to evaluate the antioxidant activity and brine shrimps assay technique to assess the cytotoxic effect.

Results and Discussion: UV-Visible spectroscopy of Hing oil mediated silver nanoparticles showed peak at 420 nm indicating the formation of silver nanoparticles. Transmission electron microscopic images of selenium nanoparticles showed the particle size ranging from 10- 55 nm.  The DPPH assay technique showed a dose dependent antioxidant activity of hing oil mediated silver nanoparticles. The antioxidant activity at 10 μl, 20 μl, 30 μl, 40 μl 50 μl was 65.8%, 68.1% 68.5% 69.1% 99.5%  respectively. However, cytotoxic activity was not here with the hing oil mediated silver nanoparticle in any of the concentration used.

Conclusion: The present study has concluded that silver nanoparticles synthesized from the   Hing oil possess a very good antioxidant effect and it does not have any  cytotoxic effect because all the brine shrimps were alive. Hence it is bio-friendly and may  be used for beneficial  biomedical application.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5315

ANTI-INFLAMMATORY ACTIVITY OF SILVER NANOPARTICLES SYNTHESISED USING Andrographis paniculata AND Phyllanthus niruri | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Background: The nanostructured noble metals have gained popularity in several medical applications such as molecular imaging, drug delivery, diagnosis and treatment of various diseases. Silver nanoparticles synthesized using plant extract will be eco-friendly, simple and reliable methods offering therapeutic options which are safe, free of side effects and effective for a wide range of diseases. In our study we have chosen Andrographis paniculata and Phyllanthus niruri which are widely used in Ayurveda for problems of the stomach, GIT and to treat chronic fever.

Aim: The aim of the present study is to analyze the anti-inflammatory activity of Andrographis paniculata and Phyllanthus niruri on plant mediated silver nanoparticles

Materials and Method: Andrographis paniculata and Phyllanthus niruri plant leaves were collected and extract was prepared. 1 Milli molar of silver nitrate was added to 90 ml double distilled water and to this 10 ml of prepared plant extract was added.  The synthesized stable silver nanoparticles were characterized by UV- Vis spectroscopy, which showed shift peak at 550 nm. The anti-inflammatory activity of silver nanoparticles synthesized using plant extract was investigated by albumin denaturation assay.

Results: The silver nanoparticles synthesized using the plant extract shows that the maximum inhibition obtained was 62% at 100 µg/ml which is comparatively less than the standard diclofenac sodium.

Conclusion: The silver nanoparticles synthesized using the plant extract acts as a potent anti-inflammatory agent. It can be recommended as a potential anti-inflammatory agent for further in-depth pharmacological studies in vivo and can be used as a replacement to the commercially available drugs.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5314