ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF Acalypha indica MEDIATED SELENIUM NANOPARTICLES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Nanoparticles are a fascinating field because they cross the gap between bulk materials and atomic structures. Nanoparticles may be made from a number of materials, including proteins, polysaccharides, and synthetic polymers, and the materials used are determined by the nanoparticle size, biocompatibility, and toxicity. Selenium nanoparticles have gotten a lot of attention because of their beneficial properties and bioactivities, such as lower toxicity than inorganic and organic forms, and they've become a popular option in nanoparticle research. Acalypha indica is a tropical annual herb with erect, sometimes simple-stemmed stems that has been recognised by locals as a valuable source of medicine for a range of therapeutic treatments. It has anti-inflammatory, antibacterial, and cancer-fighting properties. Selenium nanoparticles, on the other hand, were appealing due to their properties.

Aim: To see whether selenium nanoparticles produced by A. indica have antimicrobial and antioxidant properties.

Methodology: Antioxidant Profile by DPPH activity and Well diffusion method for antimicrobial activity were performed after the preparation of A.indica mediated Selenium nanoparticles.

A. indica mediated the results. Se nanoparticles have strong antioxidant and antimicrobial properties.

Conclusion: At high concentrations, Acalypha indica-mediated selenium nanoparticles have strong antimicrobial and antioxidant properties.

Please see the link:- https://www.ikprress.org/index.php/PCBMB/article/view/5608

ANTI INFLAMMATORY ACTIVITY OF SELENIUM NANOPARTICLES SYNTHESISED USING CLOVE AND CINNAMON: AN In vitro STUDY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Context: Synthesized from plant sources, selenium nanoparticles (Se NPs) are eco-friendly, cost-effective and have a wide variety of applications. The anti-inflammatory properties of selenium produced by different sources of herbs have been well studied. Clove and cinnamon have been used to generate Se NPs in this research.

Goal: The aim of this study was to determine the anti-inflammatory effect of the use of cloves and cinnamon in selenium nanoparticles.

Materials and methods: 0.5 gm of powdered clove and 0.5 gm of powdered cinnamon were collected and mixed with 100 ml of water. This extract is then boiled at 90 °C in a hot water bath, added 0.80 gm of selenium to this solution and subjected to continuous stirring until the brown suspension forms. The antimicrobial property against Candida albicans was evaluated and centrifuged for 10 minutes.

Results: The peak in UV spectroscopy found at 650 nm indicates the formation of selenium nanoparticles. The study found that, with an improvement in the concentration of cloves and cinnamon, selenium nanoparticles mediated the inhibition zone toward C. Albicans have been elevated. 12 mm at 150 μl was the maximum zone of inhibition collected.

Conclusion: Instead of using traditional anti-inflammatory agents to mitigate their side effects, selenium nanoparticles developed using cloves and cinnamon have strong anti-inflammatory activity and can be used in the future as pastes, gels, etc. for topical application.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5396
    

BIOGENIC SYNTHESIS OF Solanum trilobatum MEDIATED BY SELENIUM NANOPARTICLES AND ITS CYTOTOXICITY AND ANTIOXIDANT ACTIVITY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Aim: To estimate the antioxidant and cytotoxicity activity of selenium nanoparticles-mediated Solanum trilobatum.

Introduction: One of the most essential medicinal plants emerging in the southern regions of India is Solanum trilobatum, commonly called thuthuvalai. It is traditionally used in the treatment of different ailments. Certain illnesses such as tuberculosis, respiratory disorders and bronchial asthma are said to be healed. Phytochemical analysis of different extracts, such as chloroform, S. water. The existence of secondary metabolites such as steroids, triterpenoids, carbohydrates, sugar reducers and phenolic compounds was demonstrated by trilobatum. S. Antimicrobial activity, cytotoxicity activity, protective effect, haemolytic activity are said to be possessed by trilobatum.

Materials and Methods: The plant extract was prepared and the UV-Beckmann spectrometer was used to synthesise nanoparticles. The nanoparticles that were synthesised were then centrifuged. Following centrifugation, pellet collection was completed. This has been used to test various properties, such as antioxidants and cytotoxicity. The antioxidant property was tested as a normal solution using a DPPH assay where ascorbic acid was used. Wells were used for the cytotoxicity operation, where the mortality rate of the shrimps was calculated when selenium nanoparticle-mediated plant extracts of different concentrations were applied.

Results: In the case of cytotoxicity activity, solanum trilobatum induced by selenium nanoparticles showed elevated LD 50 in higher concentrations. In comparison with regular ascorbic acid, the plant extract mediated selenium nanoparticle had a major impact on antioxidant function.

Conclusion: Therefore, the selenium nanoparticle mediated by Solanum trilobatum expresses strong antioxidant activity and potent cytotoxicity. Hence, for further study, it can be taken up.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5312