In vitro ANTI-INFLAMMATORY ACTIVITY USING FRUIT FORMULATION MEDIATED ZINC OXIDE NANOPARTICLES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 The aim of this study was to see whether zinc oxide nanoparticles made from Punica granatum and Elettaria cardamomum had anti-inflammatory properties.

Background: Non-steroidal anti-inflammatory drugs (NSAIDs) such as aspirin, diclofenac, and ibuprofen are the most widely prescribed and used drugs for inflammation and its related conditions. Unfortunately, these medications have a lot of negative side effects. As a result, there has been an increase in the quest for a more powerful and secure alternative. To minimise the side effects, zinc oxide nanoparticles, which have strong anti-inflammatory efficacy, were prepared using a green method mediated by extracts from Punica granatum and Elettaria cardamomum.

Materials and Methods: Punica granatum and Elettaria cardamomum extracts were collected and prepared, zinc oxide nanoparticles were synthesised, nanoparticles were collected using centrifugation, nanoparticles were characterised, anti-inflammatory activity of ZnO nanoparticles was determined using ultraviolet-visible spectroscopy, and albumin denaturation inhibition was tested.

Results: Zinc oxide nanoparticles induced by Punica granatum and Elettaria cardamomum demonstrated anti-inflammatory activity comparable to Diclofenac.

Conclusion: The findings of the study indicate that zinc oxide nanoparticles induced by Punica granatum and Elettaria cardamomum have potent anti-inflammatory properties, and more studies can be done to test it for various medicinal purposes.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5628

Symplocos racemosa BARK MEDIATED COPPER NANOPARTICLES AND ITS ASSESSMENT OF ANTI-INFLAMMATORY ACTIVITY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 To make copper nanoparticles out of Lodhra bark and test their anti-inflammatory properties. Copper nanoparticles are antifungal and antibacterial agents that are extremely tiny. The copper free radicals then oxidise lipids in cell membranes, causing the membranes to degenerate. As a consequence of the degraded membranes, intracellular compounds leak out of cells, resulting in cell death. The local reaction of living mammalian tissues to injury is inflammation. It's a natural defence mechanism designed to stop or slow the spread of harmful agents. 1 g of freshly collected Lodhra bark is extracted and thoroughly mixed with 100 ml of distilled water, then boiled for 3 to 5 minutes in a heating mantle, filtered through filter paper, and held in a shaker. For nanoparticle synthesis, copper sulphate was combined with plant extract and kept in a shaker. Albumin denaturation assay was used to assess the anti-inflammatory function. The formation of copper nanoparticles mediated by lodhra bark was visually observed and conformed using UV visible spectroscopy. Copper nanoparticles at different concentrations have demonstrated dose-dependent inflammatory activity. In contrast to the regular 90 percent, a 50 micro lite inhibition shower has a maximum inhabitation of 81 percent. Copper nanoparticles mediated by Lodhra bark were found to be cost-effective and simple to make, with excellent anti-inflammatory properties. As a result, it will be useful in a variety of medical fields in the future.

Please see the link:- https://www.ikprress.org/index.php/PCBMB/article/view/5610

GREEN SYNTHESIS, ANTI INFLAMMATORY AND ANTIOXIDANT PROPERTY OF Cardiospermum halicacabum MEDIATED COPPER NANOPARTICLE | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Introduction: The implications of biosynthesis of nanomaterials to be incorporated with naturally accessible substances are being evaluated in recent developments in nano biomedicine, an evolving area of medicine. Green synthesis of Copper nanoparticles using plant compounds appears to be a promising method for drug production. This field of research has proved to be very cost effective and satisfactory. The anti-inflammatory and antioxidant properties of Cardiospermum halicacabum induced copper nanoparticles will be evaluated in this review.

The aim of this analysis was to determine the anti-inflammatory and antioxidant properties of copper nanoparticles mediated by C.halicacabum.

Materials and Methods: The approach involves a green synthesis of copper nanoparticles mediated by C.halicacabum, accompanied by anti-inflammatory and antioxidant activity studies.

Results: The anti-inflammatory and anti-oxidant activity of the copper nanoparticle mediated by C.halicacabum increased proportionately with increasing dose concentration.

Conclusion: Copper nanoparticles' wide applicability in a variety of fields, including medicinal, mechanical, and electronic, has fueled research into copper nanoparticles that provide a more direct path of action and are more cost effective. As a result of their cost effectiveness and lack of toxicity, green methodologies have gained more interest and excitement so far. As a result, the properties have changed. Copper nanoparticles mediated by C. halicacabum showed important anti-inflammatory and anti-oxidation activity, making them potent anti-inflammatory and antioxidant agents.

Please see the link:- https://www.ikprress.org/index.php/PCBMB/article/view/5604

ANTI INFLAMMATORY ACTIVITY OF CORIANDER OLEORESIN MEDIATED SELENIUM NANOPARTICLES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

The aim of this study was to assess the anti-inflammatory activity of selenium nanoparticles mediated by Coriander Oleoresin.

Materials and Methods: Coriander oleoresin-mediated Selenium nanoparticles were made by combining 0.5 mL Coriander Oleoresin extract with 0.519 g sodium selenite in 99.5 mL distilled water. For nanoparticle synthesis, the reaction mixture was held in an orbital shaker with magnetic stirrer. Color shift indicated the formation of selenium nanoparticles, which was confirmed using a UV-Visible spectrophotometer. The antiinflammatory activity of coriander oleoresin mediated selenium nanoparticles was assessed using an albumin denaturation inhibitory assay.

Discussion and Conclusions: The presence of selenium nanoparticles was demonstrated by a colour shift in the reaction mixture, which was verified by UV-Visible spectroscopy with a peak at 320nm. The anti-inflammatory activity of coriander oleoresin mediated selenium nanoparticles was dose dependent. At 50 L of selenium nanoparticles, the maximum inhibitory effect of 90 percent on protein denaturation was observed.

Conclusion: The synthesis of selenium nanoparticles in a green manner was simple and straightforward. The researchers concluded that Coriander Oleoresin-mediated Selenium nanoparticles had strong anti-inflammatory activity and thus could be used for a healthy and environmentally friendly selenium nanoparticle synthesis for inflammatory conditions.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5543

ANTI-INFLAMMATORY ACTIVITY OF TULASI AND TURMERIC MEDIATED COPPER NANOPARTICLES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Introduction: The objective is to understand the anti-inflammatory activity of medical copper nanoparticles provided by turmeric and Tulsi. Turmeric is a perennial, rhizomatous, herbaceous plant that requires temperatures between 20 and 30 ° C (68 and 86 ° F) and a substantial amount of annual rainfall to thrive, native to the Indian subcontinent and Southeast Asia. For their rhizomes, plants are gathered each year, some for propagation in the following season and some for consumption. Curcumin, a turmeric material, may help reduce body inflammation, so it has been widely marketed as a supplement, turmeric has also been applied to the skin for pain or swelling, and essential oil has also been added. The principal active ingredient in turmeric is curcumin. It has strong anti-inflammatory effects and is an antioxidant that is very effective. There are several medicinal properties in the Basil or Tulsi plant (Ocimum tenuiflorum). The leaves reinforce the stomach and assist with respiratory problems.

Materials and Methods: In 100 ml of distilled water, 0.5 grams of Tulasi and 0.5 grams of turmeric were added. Some characteristic color shift was observed in the extract and anti-inflammatory activity of copper nanoparticle mediated by Tulsi and Turmeric nanoparticle after addition of copper sulphate and centrifugation, and anti-inflammatory activity was measured using the UV spectrophotometer.

Results: This demonstrates that medicinal copper nanoparticles of turmeric and tulsi formation have strong anti inflammatory properties.

Conclusion: This concludes that Tulasi and turmeric combined with copper extract have shown better anti-inflammatory activity outcomes.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5519

ANTIINFLAMMATORY ACTIVITY OF ZINC OXIDE NANOPARTICLES SYNTHESISED USING GRAPE SEED EXTRACT: AN in vitro STUDY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Background: Inflammation is the key finding in any periodontal problem. It is important to devise new topical agents to reduce the inflammation. Zinc oxide nanoparticle synthesized from plant extract will be an eco-friendly, simple and reliable method offering therapeutic options which are safe, free of side effects and effective for a wide range of diseases. In our study we have chosen grape seed due to its protection against oxidative damage, and anti-diabetic, anti-cholesterol and antiplatelet properties.

Aim: The aim of the present study was to analyze the anti-inflammatory activity of zinc oxide nanoparticles synthesized using grape seed extract.

Materials and Methods: Collection of grape seeds and preparing its extract followed by the synthesis of zinc nanoparticles using the prepared herbal formulation. The stable zinc nanoparticles were characterized by UV- Vis spectroscopy, which showed shift peak at 660 nm. The anti-inflammatory activity of zinc nanoparticles prepared using plant extract was investigated by albumin denaturation assay. The results of the test were described as the standard deviation (SD) and analyzed using the UV- Vis Spectroscopy.

Results: The zinc oxide nanoparticles synthesized using the grape seed extract showed highest absorbance at a concentration of 50 μl (84.6%) when subjected to inhibition of albumin denaturation assay to check for its anti-inflammatory activity.

Conclusion: The zinc oxide nanoparticles synthesized using the grape seed extract acts as a potent anti-inflammatory agent with least side effects. Further clinical trials need to be done in the future and can be used as a replacement to the commercially available drugs.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5364

ANTI-INFLAMMATORY ACTIVITY OF SILVER NANOPARTICLES SYNTHESISED USING Andrographis paniculata AND Phyllanthus niruri | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Background: The nanostructured noble metals have gained popularity in several medical applications such as molecular imaging, drug delivery, diagnosis and treatment of various diseases. Silver nanoparticles synthesized using plant extract will be eco-friendly, simple and reliable methods offering therapeutic options which are safe, free of side effects and effective for a wide range of diseases. In our study we have chosen Andrographis paniculata and Phyllanthus niruri which are widely used in Ayurveda for problems of the stomach, GIT and to treat chronic fever.

Aim: The aim of the present study is to analyze the anti-inflammatory activity of Andrographis paniculata and Phyllanthus niruri on plant mediated silver nanoparticles

Materials and Method: Andrographis paniculata and Phyllanthus niruri plant leaves were collected and extract was prepared. 1 Milli molar of silver nitrate was added to 90 ml double distilled water and to this 10 ml of prepared plant extract was added.  The synthesized stable silver nanoparticles were characterized by UV- Vis spectroscopy, which showed shift peak at 550 nm. The anti-inflammatory activity of silver nanoparticles synthesized using plant extract was investigated by albumin denaturation assay.

Results: The silver nanoparticles synthesized using the plant extract shows that the maximum inhibition obtained was 62% at 100 µg/ml which is comparatively less than the standard diclofenac sodium.

Conclusion: The silver nanoparticles synthesized using the plant extract acts as a potent anti-inflammatory agent. It can be recommended as a potential anti-inflammatory agent for further in-depth pharmacological studies in vivo and can be used as a replacement to the commercially available drugs.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5314

GREEN SYNTHESIS OF Solanum trilobatum MEDIATED SELENIUM NANOPARTICLES AND ITS ANTI-INFLAMMATORY AND ANTI-MICROBIAL ACTIVITY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Goal: The aim of the present study is to assess the anti-inflammatory and anti-microbial activity of selenium nanoparticles mediated by Solanum trilobatum.

Materials and Methods: 1g of Solanum trilobatum powder with 0.519 g of sodium selenite with 50 ml of distilled water was used to prepare the plant extract. They then collected the extract and held it in a laboratory shaker for a few hours to synthesise selenium nanoparticles. The colour shift was detected every two hours and the readings were taken using a UV spectrometer.

Results and Discussion: Selenium nanoparticles showed a higher percentage of protein denaturation inhibition at 50 μL due to the anti-inflammatory impact. If the concentration increases, the inhibition percentage simultaneously increases. Effective inhibition against Streptococcus mutans, Lactobacillus sp and Candida albicans was shown by the anti-microbial activity of Solanum trilobatum mediated selenium nanoparticles. In our research, antimicrobial activity showed a higher-than-standard inhibition zone against S. Lactobacillus and mutans. In our research, antifungal activity showed an inhibition zone against Candida albicans almost identical to the norm used.

Conclusion: Due to the altered and outstanding properties of nanoscale materials, nanoscience and nanotechnology have numerous promising applications. The current study has shown that SeNPs controlled by Solanum trilobatum have excellent anti-inflammatory and antimicrobial activity. So this present study will provide understanding and pave a way for new medicines to be developed that could replace the norm in the future.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5311

ANTI-INFLAMMATORY ACTIVITY OF TITANIUM DIOXIDE NANOPARTICLES SYNTHESISED USING GRAPE SEED EXTRACT: AN in vitro STUDY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Background: Titanium dioxide nanoparticles (TiO2 NPs) synthesized from plant sources are eco-friendly, cost effective and have a wide range of applications. The anti-inflammatory property of titanium dioxide produced by various herbal sources has well been studied. Grape seeds have been used in this study to produce TiO2 NPs due to their anti-inflammatory, anti-diabetic, anti-cholesterol properties.

Aim: The aim of this study was to determine the anti-inflammatory activity of titanium dioxide nanoparticles synthesised using grape seed extract.

Materials and Methods: Preparation of grape seed extract, synthesis of titanium dioxide nanoparticles using grape seed extract in Lark refrigerated centrifuge, preparation of nanoparticles powder, characterisation using UV-Vis Spectroscopy and study of anti-inflammatory activity using Bovine serum albumin using Diclofenac sodium as control.

Results: The peak found in spectroscopy indicated the formation of titanium dioxide nanoparticles. The anti-inflammatory action of grape seed mediated TiO2 NPs increases with an increase in the concentration from 10 μl, 20 μl, 30 μl, 40 μl to 50 μl.

Conclusion: Titanium dioxide nanoparticles produced using grape seed extract have good anti-inflammatory activity and can be used in the future as pastes, gels etc. for topical application instead of using conventional anti-inflammatory agents to minimize their side effects.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5290

CHEMISTRY OF TURMERIC: A GOLDEN SPICE WITH THE MULTIFUNCTIONAL BENEFITS | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Turmeric is generally known for its use as spice in cooking. It is often considered as the king of spices.  Its use in the customary arrangement of medication for a considerable length of time and has been experimentally approved broadly. Through this review, researchers try to bring to notice the beneficial use of this naturally gifted spice called turmeric. Turmeric has always received considerable interest among the researchers/scientific community because of its medical and cosmetic properties. It is beyond doubt or debate that turmeric is being used for various reasons. Extensive researches time and again has proved that it has potential to treat many diseases. Curcumin a main ingredient of turmeric is the most effective in treatment. It is also beneficial and useful for skin treatment. It has different valuable properties such as antioxidant exercises and is helpful in conditions, for example, cancers, inflammation, etc. It likewise has antifungal, antimicrobial renal and hepatoprotective exercises. In this way, it has the potential against different cancer growth, diabetes, sensitivities, joint inflammation and other incessant and hard treatable infections. Both conventional and improved method of technology is used for producing turmeric and due to its multi benefits, day by day turmeric is occupying a major share in the world market specially with the entrance of latest technology and government support for small and medium scale industries.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5212