ANTIBACTERIAL ACTIVITY OF TAMARIND FRUIT PULP MEDIATED SELENIUM NANOPARTICLES ISOLATED FROM WOUNDS | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Background: Selenium nanoparticles (Se NPs) are gaining importance in pharmaceutical industries. Se NPs exhibit low cytotoxicity compared to Selenium (Se) compounds and they are found to possess excellent anticancer and other therapeutic activities. Wound healing is one of the major concerns among health care practitioners and scientists. Poor wound healing not only causes trauma to the patient but increases the burden of financial resources and requirement for cost effective management within the healthcare system

Aim: To do an In vitro study on the antibacterial activity of tamarind fruit pulp mediated selenium nanoparticles (SeNPs) against E. coli, Klebsiella and Pseudomonas sp. isolated from wounds.

Materials and Methods: Green synthesis of selenium nanoparticles was done using tamarind extract. Synthesised nanoparticles were confirmed by spectrophotometer. The synthesised nanoparticles were centrifuged. Three petri plates with Muller Hinton agar were prepared. Three plates were streaked with three bacterial species – one in each with E. coli, Klebsiella and Pseudomonas sp. Three wells were created. 100 μL each of tamarind mediated nanoparticle, tamarind mediated nanoparticle + antibiotic and antibiotic alone  was added in each well and ZOI was measured after 24 hours.

Results: Tamarind fruit pulp mediated SeNPs showed good antibacterial activity. Antibacterial activity of Tamarind SeNPs was better with a zone of inhibition of   30 mm compared to amoxicillin against E. coli which was 22 mm. The nanoparticle combination showed   synergistic activity with amoxicillin against Klebsiella sp. 31 mm as a zone of inhibition and comparatively less activity was shown against Pseudomonas sp. 11 mm

Conclusion: Tamarind fruit pulp mediated selenium nanoparticles showed good inhibitory activity on   E. coli, Klebsiella isolated from wounds. It showed synergistic activity with amoxicillin against Klebsiella. However, its activity against Pseudomonas was less. Therefore, Tamarind fruit pulp mediated selenium nanoparticles may be useful against infections caused by E. coli and Klebsiella.

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GENETIC DIVERSITY OF Tamarindus indica

It is very essential for plant breeders to determine genetic variation for the growth of elevated yield choice. The objective of this study was to investigate the genetic diversity of 3 tamarind samples, random exploitation of enhanced polymorphic DNA (RAPD) markers. Tamarind plants were sampled in 3 environmental zones covering domestically known fruit morphotypes. On 3000 fruits and flowers, 12 morphological descriptors were evaluated. Univariate and canonical analyses carried out on morphological descriptors revealed essential similarities and verified the difference between morphotypes as viewed by indigenous people. However, the assessment of variance components showed significant differences within morphotypes, indicating a great heterogeneity within seeds historically categorized as belonging to an equal morphotype. Quantitative descriptors should, therefore, be coupled with domestically perceived qualitative characteristics (pulp style and color) in order to cause a bunch of strong morphological discrimination. The differences discovered were significantly linked to ecological factors. The size and mass of fruits and seeds are cared for with increased wetness and decreased with aridity. Results also stated that fruit mass could be an intelligent pulp output expression, although its predictive strength varied among morphotypes. Outputs from the variance component assessment recommended that if any genetic tests were not finished, germplasm assortment should be performed by testing a mild variety of forests per morphotype to ensure that a big variety of genetic diversity is captured. 10 Random modified polymorphic DNA (RAPD) primers were used to evaluate tamarind trees ' genetic variety. At the molecular stage, genotypes that were strongly linked morphologically were discovered to be unrelated. A significant amount of intra-population variation reported in the donation research that could be used in interbreeding programs to introgress the intriguing characteristic of concern with effectiveness.

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