OPTIMIZING THE ACCLIMATIZATION PROCESS OF OIL PALM (Elaeis guineensis Jacq.) in vitro PLANTLETS DERIVED FROM THE MATURE ZYGOTIC EMBRYOS | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Oil palm has become one of the most important industrial crops for its use for various purposes, but in vitro regeneration has become a critical route for accelerating breeding programs because of its constraints on traditional propagation. But its low survival rate caused catastrophic losses for the industry during the hardening process. Therefore, we optimized the acclimatization phase for 90-day-old plantlets from mature zygotic embryos germinated in vitro in this research. Using eight soil less media and their combinations in a 1:1:1 ratio, viz., plantlets were acclimatized. HM1 (Vermiculite), HM2 (Cocopeat), HM3 (Perlite), HM4 (Vermiculite + Cocopeat + Soilrite), HM5 (Cocopeat + Perlite + Soilrite), HM6 (Cocopeat + Perlite + Soilrite), HM7 (Cocopeat + Vermiculite + Perlite) and HM8 (Cocopeat + Perlite + Soilrite) (Compost). In terms of plant morphological features such as shoot length, root length, number of leaves, leaf length and leaf width, plantlets grown in the HM4 showed better results after 60 days of growth in the soilless media among all the treatments attempted. But, except for root growth, no statistical significance was observed. However, the introduction of staggered shift to the transition environment significantly decreased the transplantation shock.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5528

Optimizing Potato (Solanum tuberosum), Plant Transplantation through Micropropogation | Asian Journal of Research in Botany

 Aims: A pot experiment was set to identify the most effective symbiotic couples during the acclimatization of Solanum tuberosum vitroplants inoculated with arbuscular mycorrhizal fungi (AMF).

Methodology: The answer to inoculation of 5 strains of MA fungi Funneliformis mosseae, Rhizophagus aggregatus, Rhizoglomus fasciculatum, Rhizoglomus intraradices, Rhizoglomus manihotis is evaluated on potato varieties Aïda, Atlas and Odessa. For each variety, the experimental device was totally randomized with 5 replicates. The experiment lasted 3 months.

Results: The F. mosseae strain has the highest mycorrhizal dependencies with 81.51% and 70% respectively in the Atlas and Aïda varieties. Adessa is less than 40% dependent on all strains of fungi tested. In the Atlas variety, the F. mosseae strain significantly stimulates the aerial biomass of the plants, which reaches 723.32 mg compared to the other mycorrhizal and control strains. The production of minitubers was stimulated by mycorrhizal fungi. In the Aïda variety, the two strains F. mosseae and R. aggregatum averaged 2.80 and 2.72 minitubers per plant, respectively. With the Atlas variety, F. mosseae and R. intraradices result in the most significant production of minitubers.

Conclusion: The different species of fungi tested do not have the same level of efficiency towards these varieties. Nevertheless, they all promote plant development and potato production compared to non-ininoculated controls. Mycorrhizal dependence is more observed with both Atlas and Aïda varieties.

Please see the link :- https://journalajrib.com/index.php/AJRIB/article/view/30122