In vitro ANTI-INFLAMMATORY ACTIVITY USING FRUIT FORMULATION MEDIATED ZINC OXIDE NANOPARTICLES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 The aim of this study was to see whether zinc oxide nanoparticles made from Punica granatum and Elettaria cardamomum had anti-inflammatory properties.

Background: Non-steroidal anti-inflammatory drugs (NSAIDs) such as aspirin, diclofenac, and ibuprofen are the most widely prescribed and used drugs for inflammation and its related conditions. Unfortunately, these medications have a lot of negative side effects. As a result, there has been an increase in the quest for a more powerful and secure alternative. To minimise the side effects, zinc oxide nanoparticles, which have strong anti-inflammatory efficacy, were prepared using a green method mediated by extracts from Punica granatum and Elettaria cardamomum.

Materials and Methods: Punica granatum and Elettaria cardamomum extracts were collected and prepared, zinc oxide nanoparticles were synthesised, nanoparticles were collected using centrifugation, nanoparticles were characterised, anti-inflammatory activity of ZnO nanoparticles was determined using ultraviolet-visible spectroscopy, and albumin denaturation inhibition was tested.

Results: Zinc oxide nanoparticles induced by Punica granatum and Elettaria cardamomum demonstrated anti-inflammatory activity comparable to Diclofenac.

Conclusion: The findings of the study indicate that zinc oxide nanoparticles induced by Punica granatum and Elettaria cardamomum have potent anti-inflammatory properties, and more studies can be done to test it for various medicinal purposes.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5628

SORBITOL ACCUMULATION INHIBITORY ACTIVITY OF Punica granatum | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Background: Sorbitol, also known as glucitol, is a sweet-tasting sugar alcohol that metabolises slowly in the human body. The polyol pathway, also known as the sorbitol-aldose reductase pathway, appears to be involved in diabetic complications, particularly microvascular damage to the retina, kidneys, and nerves. (Botanical name: Punica granatum; family: Punicaceae). Pomegranate juice reduces lipid peroxidation in type 2 diabetics and hypertensive patients, as well as systolic blood pressure and serum angiotensin converting enzyme activity.

The aim of this study was to see how effective pomegranate extract is at preventing sorbitol accumulation (Punica granatum).

Plant materials-Punica granatum extract was obtained from Green Chem Herbal extracts and formulations, Bengaluru, India, for use in the research.

The technique used to extract the above plant material was developed by Kesavanarayanan et al. in 2013.

Results: Punica granatum extract inhibited sorbitol formation significantly, with an IC50 of 48.51g/mL compared to 37.77g/mL for regular ascorbic acid.

Conclusions: Pomegranate extract appears to have an inhibitory effect on sorbitol accumulation in the body.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5620

EVALUATION OF ANTIFUNGAL ACTIVITY AND CYTOTOXIC EFFECTS OF FRUIT MEDIATED ZINC OXIDE NANOPARTICLES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

The aim of this research is to make zinc oxide nanoparticles from Punica granatum and Elettaria cardamomum, measure their antifungal activity against Candida albicans, and see how toxic they are.

Introduction: Zinc oxide nanoparticles are commonly used in medicine and science due to their inherent antimicrobial, antioxidant, and anti-inflammatory properties, as well as their low toxicity. The green synthesis of these nanoparticles is favoured because it is simple, relatively repeatable, cost-effective, and produces more stable, non-toxic materials.

Materials and Methods: Green zinc oxide nanoparticles were made from aqueous fruit extracts of P. granatum and E. cardamomum. UV spectroscopy and scanning electron microscopy were then used to determine the properties of nanoparticles. The agar well diffusion method was used to assess antifungal activity, and the brine shrimp lethality assay was used to assess cytotoxicity.

P. granatum and E. cardamomum extracts allowed the synthesis of stable zinc oxide nanoparticles, according to the findings. The nanoparticles thus produced were also found to have strong antifungal properties, with a zone of inhibition of 12mm for 50L, 22mm for 100L, and 24mm for 150L. In a lethality assay, 80 percent of live shrimp larvae were found in a nanoparticle concentration of 20-30 L. The cytotoxicity of the nanoparticles, on the other hand, needs to be investigated further. They may be integrated into various products in the future, such as toothpaste, mouthwash, and mouth paint, to promote their effect.

Please see the link:- https://www.ikprress.org/index.php/PCBMB/article/view/5582

EFFICACY OF POMEGRANATE MOUTHWASH AS AN ANTIMICROBIAL AGENT | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Background: The most common oral diseases are periodontitis and tooth decay. Pomegranate mouthwash has been used in an attempt against the same with the latest controversy in herbal medicine. The purpose of the analysis was to determine the effectiveness of pomegranate mouthwash as an antimicrobial agent.

Content and Methods: As per a previous study, the mouthwash was prepared using pomegranate seeds. This double-blinded research was conducted on a total of 20 oral prophylaxis participants from both sexes who registered to Saveetha Dental College and Hospitals, Chennai. Patients were split into two groups, with pomegranate mouthwash in group 1 and 0.2% chlorhexidine gluconate mouthwash in group 2. Oral prophylaxis was performed once the participants were enrolled as part of the analysis. After that, unstimulated saliva samples were obtained and the same for the entire bacterial colony count was cultivated in nutrient agar. The same thing repeated itself after two weeks.

Results: Within the limits of the current analysis, a decrease in the total number of colonies was observed when pomegranate mouthwash was used from 150.54 colony forming units (CFU) to 48.99 CFU compared to chlorhexidine mouthwash from 152.06 CFU to 57.7 CFU. As compared to group 2, the decline in colony count was greater in group 1.

Conclusion: The present study showed that mouthwash with pomegranate showed greater antimicrobial activity than mouthwash with chlorhexidine. After long-term clinical trials, the prospective use of pomegranate as a convenient alternative to antimicrobial drugs should be considered.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5293