GREEN PREPARATION OF NANO COPPER (Cu) WITH NANO GRAPHENE OXIDE (GO) NANO COMPOSITE CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY AGAINST ORAL AEROBIC PATHOGENS | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Background: Because it does not use any harmful agents, green nanoparticle synthesis is a cost-effective and safe method of synthesis. Copper is known for its antibacterial properties, and graphene oxide is known for its barrier and structural strength, so these materials were selected to make a nanocomposite.

The study's goal was to combine green nano copper preparation and characterization with nano graphene oxide nanocomposite characterization and antimicrobial activity.

Materials and Methods: This is an in vitro experiment. A green preparation technique was used to create the nanocomposite. Copper and graphene oxide nano-composite were made using extract from the amla fruit. A TEM scan and UV-Visible spectrometric analysis were used to characterise the prepared copper and graphene oxide nanocomposite. The antimicrobial activity was tested against the microorganisms that are most commonly found in the mouth. For the antimicrobial evaluation of nanocomposite, inhibition zones were measured for Enterococcus faecalis, Streptococcus mutans, and Candida albicans at different concentrations.

At a concentration of 150 L, the nanocomposite demonstrated good antimicrobial activity against three oral aerobic microbes.

Conclusion: Within the scope of the study, copper and graphene oxide nanocomposite has promising antibacterial properties, but more cytotoxicity studies are needed before the composite can be incorporated into dental materials.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5550

ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF TAMARIND MEDIATED COPPER OXIDE NANOPARTICLES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Objective: To determine the antioxidant and antibacterial function of nanoparticles induced by Tamarind Copper Oxide.

Materials and methods: In 50 mL of distilled water, 0.318 g of CuSO4 powder was dissolved and 50 mL of the prepared extract was applied to it. The solution was deposited in a nanoparticle-forming orbital shaker and checked by a UV-Visible spectrophotometer. The antibacterial activity of the agar well diffusion system against S was assessed. Lactobacillus and mutans. The antioxidant function of the copper oxide nanoparticle induced by tamarind was evaluated by the DPPH assay technique.

Results: Nanoparticles of copper oxide showed a concentration - dependent antibacterial activity against S. Mutans versus Lactobacillus. The concentration-dependent antioxidant activity was shown by the different concentrations of Tamarind-mediated copper oxide nanoparticles used (10 μL, 20 μL, 30 μL, 40 μL, 50 μL). Copper oxide nanoparticles have demonstrated the highest radical scavenging activity at a concentration of 50 μL.(94.7 percent ).

Conclusion: Copper Oxide nanoparticles synthesized using tamarind showed strong antibacterial and antioxidant activity and can therefore be used as a healthy, simple and eco-friendly green synthesis of copper nanoparticle for its antibacterial and antioxidant properties.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5523