SYNTHESIS, CHARACTERISATION AND ANTI-INFLAMMATORY PROPERTY OF HYDROXYCITRIC ACID MEDIATED ZINC NANOPARTICLES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Aim: To evaluate hydroxy citric acid mediated ZnNp and it’s anti-inflammatory and antioxidant effects.

Introduction: Hydroxy citric acid is a chemical that is found in fruit rinds of Garcinia cambogia, Garcinia indica, and Garcinia atroviridis, but very abundant in cambogia species. Hydroxy citric acid is used to improve exercise performance and weight loss. Zinc nanoparticles have a large surface area relative to their size and high catalytic activity. Hydroxycitric acid [HCA] is a derivative of citric acid that is found in a variety of tropical plants including Garcinia cambogia and Hibiscus sabdariffa. The use of HCA is contraindicated in patients suffering Colitis or Inflammatory Bowel Disease.

Materials and Methods: Biosynthesis and characterization of Hydroxycitric acid mediated Zinc Nanoparticle using Transmission Electron Microscope (TEM) and UV-vis spectroscopy, to evaluate its biological property Anti Inflammatory activity was analyzed by protein denaturation assay.

Results and Discussion: Hydroxycitric acid in zinc nanoparticles had a nanoparticle size ranging from 10-100 nm and it also showed a very good anti-inflammatory activity when compared with that of the standard anti-inflammatory drug.

Conclusion: The study concludes that hydroxy citric acid mediated in zinc nanoparticles shows a very good anti-inflammatory property and further research could be carried out for further investigations of hydroxy citric acid mediated in zinc nanoparticles in Pharmacology.

Please see the link:- https://www.ikprress.org/index.php/PCBMB/article/view/5601

GREEN SYNTHESIS OF COPPER OXIDE NANOPARTICLES USING TAMARIND EXTRACT AND ITS ALPHA AMYLASE INHIBITORY ACTIVITY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Aim: To synthesize copper oxide nanoparticles (CuONP) using tamarind extract, and determine the in-vitro α- Amylase inhibitory activity of the CuONP.

Introduction: Tamarindus indica L., commonly known as Tamarind tree, Leguminosae (Fabaceae), is one of the most important multipurpose tropical fruit found in Indian subcontinent. Plant materials are recently used for the synthesis of nanoparticles as it is eco-friendly. Tamarind being a versatile plant with many known activities was used in this study for the synthesis of CuO NP. The in vitro α- amylase inhibitory activity was also assessed.

Materials and Methods: Copper oxide nanoparticle was synthesised using copper sulphate. Briefly, 1.11 g of tamarind fruit pulp concentrate was mixed with 100 mL of water to obtain a solution .To prepare CuSO₄ solution_,0.318 g of CuSO₄ powder was dissolved in  50 mL distilled water and 50 ml of the previously prepared tamarind  fruit pulp solution was added to the 50 mL of CuSO4 solution and the mixture was kept in an orbital shaker for the formation of nanoparticles. The prepared tamarind fruit pulp mediated CuO NP was evaluated for its antidiabetic activity by assessing its alpha -amylase inhibitory effect. 

Results and Discussion: The formation of CuO NP was initially observed by the color change was further confirmed by UV -Visible spectrometer.  The percentage inhibition was 87.4, 88.6, 88.9, 89.1, 89.4 and 89.7 for concentrations of 10,20,30,40,50 and control (acarbose)  respectively. The tamarind fruit pulp mediated CuO NP showed a dose dependent α-amylase inhibitory activity with a maximum inhibitory of 89.6 % compared to control, acarbose, at 50 uL.

Conclusion: The green synthesis CuO NPs using tamarind fruit pulp was simple, relatively   easy and eco- friendly. The α-amylase inhibitory effect of the CuO NPs suggests its possible use as additive or adjuvant for anti-diabetic drugs because uncontrolled hyperglycemia is the major reason for later stage diabetic complications.

Please see the link:- https://www.ikprress.org/index.php/PCBMB/article/view/5600