EVALUATION OF ANTIMICROBIAL PROPERTY OF HERBAL MOUTHWASH USING CRANBERRY EXTRACT: A PILOT STUDY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Background: Dental plaque-induced oral diseases continue to affect the majority of the world's population. Among them, the single most common and preventable oral infectious disease is dental caries. As fresh fruit, juice, and even as medicine, cranberry is used. It has activities that are antimicrobial, anti inflammatory, and anti-tumor. In various systemic infections, it is also known to cause bacterial adhesion. It is less popular for its dental application, however. There is, therefore, a need to determine its effect on oral infections.

Aim: The objective of the study was to test the antimicrobial property of herbal mouthwash using cranberry extract.

Materials and Methods: This unique research was performed at Saveetha Dental College and Hospitals, Chennai, as a single blinded, parallel group clinical trial. It consisted of a total of 10 subjects randomly divided into groups with a fair gingival index (aged 18-22 years) and given 5 ml of chlorhexidine mouthwash and cranberry mouthwash twice daily to Group A (n=5) and Group B (n=5) for 7 days each. At baseline, plaque samples were obtained and the microbial count was measured after 7 days of using mouthwash.

Results and Discussion: This research indicates that the microbial count has decreased substantially before and after the use of cranberry mouthwash and chlorhexidine mouthwash. There was a statistically significant difference (p<0.05) between the baseline mean amount of CFU/ml and the chlorhexidine and cranberry groups after 7 days. For the cranberry group, the mean reduction was 52 percent, while the mean reduction after use was 53 percent in the chlorhexidine group.

Conclusion: This analysis revealed that there was a substantial decrease in the microbial count in both classes. Therefore, in order to improve oral health, herbal products such as cranberry have been shown to be more effective or similar alternatives to chlorhexidine, with added benefits and minimal side effects.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5529

COMPARING THE ANTIGINGIVITIS, ANTIPLAQUE EFFECTIVENESS OF CRANBERRY AND CHLORHEXIDINE MOUTH RINSE – A SINGLE BLIND RANDOMIZED CONTROL TRIAL | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Context: Dental caries have been known to be the single oral infectious disease most preventable and widespread. Since tooth brushing is considered to be the most common technique of oral hygiene, dentifrices are also the most ideal means of delivering antibacterial agents on a daily basis. The best anti-plaque property is found to be chlorhexidine mouthwash and is considered the gold standard. As fresh fruit, juice, and even as medicine, cranberry is used. It has functions that are antimicrobial, anti-inflammatory and anti-tumor. We can therefore see that its effect on different oral infections needs to be evaluated.

Goal: The aim of the study is to compare the 0.6 percent cranberry mouthwash against gingivitis, anti plaque against 0.2 percent chlorhexidine mouthwash in undergraduate dental students.

Materials and Methods: A single blinded, parallel group clinical trial performed at Saveetha Dental College, Chennai, was the present study. Two groups of 20 participants (aged 18-22 years) were divided randomly into Group A (n=10) and Group B (n=10) and each received 5 mL of cranberry mouthwash or chlorhexidine mouthwash twice daily for 7 days. The gingival index and plaque index were registered at the baseline and on the 7th day. Before the study started, all the participants were told about their willingness to participate in the study. In the form of mean and standard deviation, descriptive statistics were expressed. To find the normality of the data, Shapiro-test Wilk's was used. The Wilcoxon-signed Rank test was used for each category to compare the mean differences in plaque and gingival index scores before and after treatment. To estimate differences between the mean Plaque index score and mean gingival index between the classes, Mann-Whitney' U Test.

Results and Discussion: This research indicates a substantial decrease in the gingival index and plaque index before and after cranberry and chlorhexidine mouthwash use. Thus, a statistically significant decrease in mean plaque and mean gingival index scores was observed in the present study in both the baseline and 7th day classes.

Conclusion: We can conclude from this study that herbal products such as cranberry can act as effectively as chlorhexidine to improve oral hygiene with additional systemic benefits and minimal long-term side effects. In assessing the advantages and side effects of such herbal extracts, further thorough studies are needed.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5527

MICROBICIDAL EFFECT OF Emblica officinalis ON BLACK PIGMENTED BACTERIA IN PATIENTS WITH PERI-IMPLANT MUCOSITIS | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Emblica officinalis (Amla) is very rich in phenolics and is widely available in the Indian market. The strong antioxidant activity of the phenolics and their ability to protect cells against oxidative damage caused by free radicals is well established. Phenolics are the major chemical constituents of Amla and these substances have strong antioxidant properties and might contribute to the healthy effects of Amla. The purpose of this study is to check the microbicidal effect of Emblica officinalis on black-pigmented bacteria in peri-implant mucositis. The aim of the present study is to evaluate the microbicidal effect of Emblica officinalis on black-pigmented bacteria in patients with peri- implant mucositis. The microbicidal effect of Emblica officinalis was determined using a culture method. 40 patients were divided into 2 groups with 20 patients in each group. Group A received Chlorhexidine mouthwash while Group B received Amla extract. 2 separate samples were taken between duration of 5 days. The samples were obtained from the buccal surface of the implants with a periodontal probe and were transported in sterile test tubes containing 1ml saline. Trypsicated soya agar broth with hemin and menadione was used for anaerobic culture with a gas pack. Both Chlorhexidine and Amla showed a significant reduction in total bacterial count and reduction in black-pigmented bacteria. Despite the fact that the Amla extract was not a pure compound, the antimicrobial results were obtained. This recommends the potency of these extracts. The figment of the derivation of antimicrobial compounds from plants seems lucrative as it will lead to the development of a phytomedicine to act against microbes.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5356

ANTIBACTERIAL & ANTI INFLAMMATORY EFFECT OF Cassia auriculata MOUTHWASH VERSUS CHLORHEXIDINE IN PLAQUE INDUCED GINGIVITIS - A PROSPECTIVE CLINICAL & MICROBIOLOGICAL STUDY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Aim: The purpose of this study was to evaluate, through clinical and microbiological examination, the antibacterial and anti-inflammatory effect of Cassia auriculata mouthwash in Plaque induced gingivitis and to compare it with Chlorhexidine mouthwash.

Materials and Methods: With the coin toss process, thirty patients who participated in the study were randomly divided into two groups. Either herbal mouthwash or chlorhexidine was administered in each community containing 15 patients. Subgingival plaque samples were obtained at baseline and 15 days later, respectively. At baseline, and 15 days later, the Silness and Loe plaque index and Loe and Silness gingival index were reported. Patients have been asked to report any pain, changes in taste, or other side effects.

Results: The results showed that there was no statistically significant difference between group A and group B for PI and GI during the baseline period. Group B (Chlorhexidine mouthwash - control group) was substantially successful in reducing the plaque index and gingival index relative to group A after 15 days of intervention (Cassia auriculata mouthwash). The mean CFUs of group B were lower than the mean CFUs of group A at baseline and after 15 days, with a statistically significant p value of 0.001. Within group A and group B, both Cassia auriculata mouthwash and Chlorhexidine mouthwash are equally potent in significantly reducing the PI scores, GI scores, CFUs, when all these parameters were compared between baseline and day 15.

Conclusion: Cassia auriculata mouthwash among patients with plaque caused gingivitis is effective in reducing plaque, gingival inflammation and CFUs, but is not equal to that of Chlorhexidine mouthwash. However, compliance with the herbal mouthwash was safer for the patient, as the patient reported no side effects compared to chlorhexidine.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5292