SYNTHESIS, CHARACTERISATION AND ANTI-INFLAMMATORY PROPERTY OF HYDROXYCITRIC ACID MEDIATED ZINC NANOPARTICLES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Aim: To evaluate hydroxy citric acid mediated ZnNp and it’s anti-inflammatory and antioxidant effects.

Introduction: Hydroxy citric acid is a chemical that is found in fruit rinds of Garcinia cambogia, Garcinia indica, and Garcinia atroviridis, but very abundant in cambogia species. Hydroxy citric acid is used to improve exercise performance and weight loss. Zinc nanoparticles have a large surface area relative to their size and high catalytic activity. Hydroxycitric acid [HCA] is a derivative of citric acid that is found in a variety of tropical plants including Garcinia cambogia and Hibiscus sabdariffa. The use of HCA is contraindicated in patients suffering Colitis or Inflammatory Bowel Disease.

Materials and Methods: Biosynthesis and characterization of Hydroxycitric acid mediated Zinc Nanoparticle using Transmission Electron Microscope (TEM) and UV-vis spectroscopy, to evaluate its biological property Anti Inflammatory activity was analyzed by protein denaturation assay.

Results and Discussion: Hydroxycitric acid in zinc nanoparticles had a nanoparticle size ranging from 10-100 nm and it also showed a very good anti-inflammatory activity when compared with that of the standard anti-inflammatory drug.

Conclusion: The study concludes that hydroxy citric acid mediated in zinc nanoparticles shows a very good anti-inflammatory property and further research could be carried out for further investigations of hydroxy citric acid mediated in zinc nanoparticles in Pharmacology.

Please see the link:- https://www.ikprress.org/index.php/PCBMB/article/view/5601

GREEN SYNTHESIS OF COPPER OXIDE NANOPARTICLES USING TAMARIND EXTRACT AND ITS ALPHA AMYLASE INHIBITORY ACTIVITY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Aim: To synthesize copper oxide nanoparticles (CuONP) using tamarind extract, and determine the in-vitro α- Amylase inhibitory activity of the CuONP.

Introduction: Tamarindus indica L., commonly known as Tamarind tree, Leguminosae (Fabaceae), is one of the most important multipurpose tropical fruit found in Indian subcontinent. Plant materials are recently used for the synthesis of nanoparticles as it is eco-friendly. Tamarind being a versatile plant with many known activities was used in this study for the synthesis of CuO NP. The in vitro α- amylase inhibitory activity was also assessed.

Materials and Methods: Copper oxide nanoparticle was synthesised using copper sulphate. Briefly, 1.11 g of tamarind fruit pulp concentrate was mixed with 100 mL of water to obtain a solution .To prepare CuSO₄ solution_,0.318 g of CuSO₄ powder was dissolved in  50 mL distilled water and 50 ml of the previously prepared tamarind  fruit pulp solution was added to the 50 mL of CuSO4 solution and the mixture was kept in an orbital shaker for the formation of nanoparticles. The prepared tamarind fruit pulp mediated CuO NP was evaluated for its antidiabetic activity by assessing its alpha -amylase inhibitory effect. 

Results and Discussion: The formation of CuO NP was initially observed by the color change was further confirmed by UV -Visible spectrometer.  The percentage inhibition was 87.4, 88.6, 88.9, 89.1, 89.4 and 89.7 for concentrations of 10,20,30,40,50 and control (acarbose)  respectively. The tamarind fruit pulp mediated CuO NP showed a dose dependent α-amylase inhibitory activity with a maximum inhibitory of 89.6 % compared to control, acarbose, at 50 uL.

Conclusion: The green synthesis CuO NPs using tamarind fruit pulp was simple, relatively   easy and eco- friendly. The α-amylase inhibitory effect of the CuO NPs suggests its possible use as additive or adjuvant for anti-diabetic drugs because uncontrolled hyperglycemia is the major reason for later stage diabetic complications.

Please see the link:- https://www.ikprress.org/index.php/PCBMB/article/view/5600

BIOGENIC SYNTHESIS OF Solanum trilobatum MEDIATED BY SELENIUM NANOPARTICLES AND ITS CYTOTOXICITY AND ANTIOXIDANT ACTIVITY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Aim: To estimate the antioxidant and cytotoxicity activity of selenium nanoparticles-mediated Solanum trilobatum.

Introduction: One of the most essential medicinal plants emerging in the southern regions of India is Solanum trilobatum, commonly called thuthuvalai. It is traditionally used in the treatment of different ailments. Certain illnesses such as tuberculosis, respiratory disorders and bronchial asthma are said to be healed. Phytochemical analysis of different extracts, such as chloroform, S. water. The existence of secondary metabolites such as steroids, triterpenoids, carbohydrates, sugar reducers and phenolic compounds was demonstrated by trilobatum. S. Antimicrobial activity, cytotoxicity activity, protective effect, haemolytic activity are said to be possessed by trilobatum.

Materials and Methods: The plant extract was prepared and the UV-Beckmann spectrometer was used to synthesise nanoparticles. The nanoparticles that were synthesised were then centrifuged. Following centrifugation, pellet collection was completed. This has been used to test various properties, such as antioxidants and cytotoxicity. The antioxidant property was tested as a normal solution using a DPPH assay where ascorbic acid was used. Wells were used for the cytotoxicity operation, where the mortality rate of the shrimps was calculated when selenium nanoparticle-mediated plant extracts of different concentrations were applied.

Results: In the case of cytotoxicity activity, solanum trilobatum induced by selenium nanoparticles showed elevated LD 50 in higher concentrations. In comparison with regular ascorbic acid, the plant extract mediated selenium nanoparticle had a major impact on antioxidant function.

Conclusion: Therefore, the selenium nanoparticle mediated by Solanum trilobatum expresses strong antioxidant activity and potent cytotoxicity. Hence, for further study, it can be taken up.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5312

ANTI-FUNGAL ACTIVITY OF CHITOSAN NANOPARTICLE INCORPORATED LYCOPENE AGAINST Candida albicans USING MINIMAL INHIBITORY CONCENTRATION ASSAY

 Background: Recent years have witnessed growth in the research and application of nanoparticles and nanotechnoloy. Lycopene is a red coloured pigment found in fruits and vegetables. Chitosan is a straight chain polymer composed of glucosamine and N-acetylglucosamine. Both the substances possess antifungal activity. Hence, the aim of this study was to assess the antifungal activity of lycopene extract, chitosan nanoparticle and lycopene chitosan nanocomposite.

Materials and Methods: Chitosan nanoparticle incorporated lycopene was prepared and its antifungal activity was tested individually and in combination against Candida albicans using minimum inhibitory concentration assay. Chitosan solution was prepared, centrifugation was done and further characterisation of chitosan nanoparticle was done using Ultaviolet-viz-Spectroscopy. The antifungal activity was assessed using minimum inhibitory concentration.

Results:  The present study shows that the antifungal effect of chitosan-lycopene nanocomposite was higher than lycopene extract and chitosan nanoparticle individually. The antifungal activity of lycopene chitosan nanocomposite increased with increase in concentration and time.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5295