Symplocos racemosa BARK MEDIATED COPPER NANOPARTICLES AND ITS ASSESSMENT OF ANTI-INFLAMMATORY ACTIVITY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 To make copper nanoparticles out of Lodhra bark and test their anti-inflammatory properties. Copper nanoparticles are antifungal and antibacterial agents that are extremely tiny. The copper free radicals then oxidise lipids in cell membranes, causing the membranes to degenerate. As a consequence of the degraded membranes, intracellular compounds leak out of cells, resulting in cell death. The local reaction of living mammalian tissues to injury is inflammation. It's a natural defence mechanism designed to stop or slow the spread of harmful agents. 1 g of freshly collected Lodhra bark is extracted and thoroughly mixed with 100 ml of distilled water, then boiled for 3 to 5 minutes in a heating mantle, filtered through filter paper, and held in a shaker. For nanoparticle synthesis, copper sulphate was combined with plant extract and kept in a shaker. Albumin denaturation assay was used to assess the anti-inflammatory function. The formation of copper nanoparticles mediated by lodhra bark was visually observed and conformed using UV visible spectroscopy. Copper nanoparticles at different concentrations have demonstrated dose-dependent inflammatory activity. In contrast to the regular 90 percent, a 50 micro lite inhibition shower has a maximum inhabitation of 81 percent. Copper nanoparticles mediated by Lodhra bark were found to be cost-effective and simple to make, with excellent anti-inflammatory properties. As a result, it will be useful in a variety of medical fields in the future.

Please see the link:- https://www.ikprress.org/index.php/PCBMB/article/view/5610

GREEN SYNTHESIS, ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY OF Adathoda vasica MEDIATED COPPER NANOPARTICLES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Introduction: Adhathoda vasica is a native plant that has a wide variety of disease-fighting properties. Copper nanoparticles have a number of advantages, including a high yield reaction. The combination of these two magnificent materials has the potential to improve antioxidant and anti-inflammatory function. The main aim of this research is to determine the antioxidant and anti-inflammatory activity of the A. vasica plant extract using copper nanoparticles. Both have a unique property that, due to its supra additive effect, can produce an even more successful result.

Aim: To establish the antioxidant and anti-inflammatory properties of Copper nanoparticles mediated by A.vasica.

Materials and Methods: Adhatoda vasica is used to make copper nanoparticles. Following the synthesis, centrifugation was carried out for a few minutes. Following the process, the pellets were separated and chosen separately. The antioxidant and anti-inflammatory activity of copper nanoparticles mediated by A. vasica nanoparticles was then assessed using this method. A UV spectrophotometer was used to test anti-inflammatory and antioxidant activity.

The copper nanoparticles mediated by A. vasica have antioxidant and anti-inflammatory properties. Copper nanoparticles have a powerful catalytic capacity that can be used to treat inflammation. This plant extract is also used to treat chronic colds and coughs. The aim of this study was to find a better combination of copper nanoparticles regulated by A. vasica that would express its antioxidant and anti-inflammatory functions while also preventing disease.

Conclusion: This study found that copper nanoparticles mediated by A. vasica have strong antioxidant and anti-inflammatory activity at high concentrations close to those found in standard drugs.

Please see the link:- https://www.ikprress.org/index.php/PCBMB/article/view/5606

GREEN SYNTHESIS, ANTI INFLAMMATORY AND ANTIOXIDANT PROPERTY OF Cardiospermum halicacabum MEDIATED COPPER NANOPARTICLE | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Introduction: The implications of biosynthesis of nanomaterials to be incorporated with naturally accessible substances are being evaluated in recent developments in nano biomedicine, an evolving area of medicine. Green synthesis of Copper nanoparticles using plant compounds appears to be a promising method for drug production. This field of research has proved to be very cost effective and satisfactory. The anti-inflammatory and antioxidant properties of Cardiospermum halicacabum induced copper nanoparticles will be evaluated in this review.

The aim of this analysis was to determine the anti-inflammatory and antioxidant properties of copper nanoparticles mediated by C.halicacabum.

Materials and Methods: The approach involves a green synthesis of copper nanoparticles mediated by C.halicacabum, accompanied by anti-inflammatory and antioxidant activity studies.

Results: The anti-inflammatory and anti-oxidant activity of the copper nanoparticle mediated by C.halicacabum increased proportionately with increasing dose concentration.

Conclusion: Copper nanoparticles' wide applicability in a variety of fields, including medicinal, mechanical, and electronic, has fueled research into copper nanoparticles that provide a more direct path of action and are more cost effective. As a result of their cost effectiveness and lack of toxicity, green methodologies have gained more interest and excitement so far. As a result, the properties have changed. Copper nanoparticles mediated by C. halicacabum showed important anti-inflammatory and anti-oxidation activity, making them potent anti-inflammatory and antioxidant agents.

Please see the link:- https://www.ikprress.org/index.php/PCBMB/article/view/5604

ANTI-INFLAMMATORY ACTIVITY OF COPPER NANOPARTICLES SYNTHESIZED USING DRIED GINGER | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Inflammation is an immune system biochemical response that can be activated by a wide range of factors, including bacteria, damaged cells, and toxic compounds. The Zingiberaceae family includes ginger (Zingiber officinale). Ginger's phytochemistry is responsible for its health-promoting properties. Ginger is also said to have anti-inflammatory and antioxidative properties that help to slow down the ageing process. It also has antimicrobial properties that can help in infectious disease treatment. Oxidative stress occurs when free radicals or reactive oxygen species (ROS) are generated in excess of the biological system's antioxidant capacity throughout metabolism. The aim of this study is to see how effective copper nanoparticles are at reducing inflammation when they are combined with dried ginger. The Zingiber officinale was harvested, peeled, washed, and air dried in a shady spot, after which a plant extract was produced and copper nanoparticles were made. The synthesis of copper nanoparticles was monitored using a UV-Vis spectrophotometer on a regular basis. The inhibition of albumin denaturation assay test was used to assess anti-inflammatory activity, and the results revealed that dried ginger mediated copper nanoparticles had potent anti-inflammatory properties.

Please see the link:- https://www.ikprress.org/index.php/PCBMB/article/view/5602

CYTOTOXICITY OF COPPER NANOPARTICLES SYNTHESIZED USING DRIED GINGER | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Copper nanoparticles are used in a wide range of fields, including medicine, agriculture, and engineering, to name a few. This biotechnology incorporates biological concepts of physical and chemical properties to create nano-sized particles with unusual properties. The quality of being harmful to cells is known as cytotoxicity. The goal of this study was to look at the cytotoxicity of copper nanoparticles made from dried ginger. Copper nanoparticles were synthesised using a green method and characterised using UV-VIS spectroscopy. The brine shrimp lethality assay was used to assess the cytotoxicity of the dried ginger induced copper nanoparticles at various concentrations. The findings showed that copper nanoparticles made from dried ginger had lower toxicity when used at lower concentrations. As a result, different nano-formulations of dried ginger mediated copper nanoparticles can be produced at optimal concentrations for safe and cost-effective therapeutics.

Please see the link:- https://www.ikprress.org/index.php/PCBMB/article/view/5584

GREEN SYNTHESIS OF COPPER NANOPARTICLES USING TURMERIC-TULSI EXTRACT AND ITS CHARACTERIZATION | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 The eco-friendly and cost-effective green synthesis of copper nanoparticles using plant extracts and enzymes does not require high pressure, energy, temperature, or other toxic chemicals. Green synthesis of nanoparticles is an emerging field of research that is preferred over physical or chemical methods because it is more cost-effective, non-toxic, and environmentally friendly. Copper nanoparticles were synthesised with turmeric-tulsi extract and characterised using UV-visible spectroscopy and Transmission Electron Microscopy in this study (TEM). Traditional medicinal plants like turmeric and tulsi are high in beneficial phytocompounds that can help nanoparticles form. The stability of copper nanoparticles synthesised from these extracts was investigated using UV-vis spectroscopy. The morphology of the synthesised copper nanoparticles was determined using TEM. This is a novel study in which copper nanoparticles were easily synthesised in an environmentally friendly way using turmeric-tulsi extract, and it could be of critical significance in the rapidly evolving world of medical diagnosis and treatment.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5580

ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF Celastrus paniculatus MEDIATED COPPER NANOPARTICLES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Aim: The research aims to evaluate the copper nanoparticles mediated by Celastrus paniculatus and their antimicrobial and antioxidant function.

Presentation: C. A member of the family Celastraceae, paniculatus is a large, woody climber with a corky yellow bark. It is found throughout India and has been recorded to grow to almost 2000 m in height. There are varying degrees of therapeutic values in plants, some of which are used to treat cognitive dysfunction, epilepsy, insomnia, rheumatism, gout, dyspepsia. The most widely used plant portion is oil from seeds called Jyothismati oil, which contains the alkaloids celastrine and paniculatin in various quantities.

Materials And Methods: To assess the antibacterial activity of copper nanoparticles, the agar well diffusion process was used. Streptococcus mutans, Candida albicans and Lactobacillus spp. were screened against different concentrations of CuNPs. As a positive monitor, antibiotics were used. Inhibition zones were reported on each plate. In 5 test tubes ranging from 10 to 50 microliters and held in a dark place for 10 minutes, 50 percent methanol, DPPH solution and CuNP were added for antioxidant activity and the reading was noted.

Results: As a consequence, a dose-dependent incremental increase in activity of S is observed. C. Mutans. Lactobacillus spp., Albicans. As compared to the normal and antioxidant activity, 150 microliters were observed in 50 microliters where the percentage of inhibition was maximum.

Conclusion: As a consequence of this, it is inferred that C. Copper nanoparticles mediated by paniculates have a strong antimicrobial and antifungal activity, and it also has a good antioxidant impact.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5336

ANTIFUNGAL ACTIVITY OF COPPER NANOPARTICLES SYNTHESISED USING Symplocos racemosa BARK | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Introduction: The combination of metal nanoparticles is a rising region of research and innovation with potential application in plant insurance. Symplocos racemosa has various uses such as stimulating appetite, good for eyes, helps in digestion and used in cough and acidity. In the present investigation, with an eco-accommodating methodology, an advantageous strategy was embraced, where copper nanoparticles are biosynthesised extracellularly by utilising Symplocos racemosa. Nanoparticles have been used in various practical applications. Copper nanoparticles act as good antibacterial agents also. Copper nanoparticles are used widely due to their heat transfer process such as high thermal conductivity.

Materials and Methods: Lodhra bark “(Symplocos racemosa)” was collected from the market and copper nanoparticles were synthesised using Symplocos racemosa. It was mixed with 100 ml of distilled water thoroughly and boiled for 3-5 mins in a heating mantle then filtered using filter paper. CuSO4 solution was added and lodhra bark was extracted. The solution is kept in a shaker and reading was taken for every 2 hours.

Results: There are various methods to prepare copper nanoparticles and those methods are greener, environment suitable, cheap and also best compared to other conventional methods. It has high surface area, low production cost, catalytic activity compared to other precision metals. From the graph obtained, we can observe that copper nanoparticles have significant antifungal activity.

Conclusion: Therefore it can be concluded that copper nanoparticles have significant antifungal activity especially when it is synthesised using Symplocos racemosa, it can be used as a better drug of choice.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5327