CONTROLLING Streptococcus mutans USING SELENIUM NANOPARTICLES MEDIATED THROUGH Symplocos racemosa | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 When left unsupervised, Streptococcus mutans (Sm), the harbinger of dental decay, is a pathogenic bacterium that has been linked to the pathogenesis of cardiovascular diseases. With the right inhibitory measures in place, we can reduce its role in exacerbating morbidity. The goal of our research was to look into the antibacterial potential of selenium nanoparticles (Se-NPs) made from Symplocos racemosa (Sr).

Sr was pulverised and turned into a filtered herbal formulation. For the synthesis of nanoparticles, sodium selenite solution was added to it, and the mixture was kept in a magnetic stirrer. UV-vis spectroscopy was used to characterise the Se-NPs, which were then centrifuged until the final pellet was obtained. On a Muller-Hinton Agar plate, a fresh bacterial suspension of Sm was scattered. The plate was incubated at 37°C for 24 hours with Se-NP concentrations of (50, 100, and 150) l in each well. The zones of inhibition were measured using antibiotics as a control.

Following the synthesis of Se-NPs, there was a visible colour change. UV-vis spectroscopy was used to characterise the prepared particles, and a peak at 280 nm was discovered. With an increase in Se-NP concentration, the inhibition zones grew larger. The antibacterial control was followed by 150 L of our Se-NPs, which was the most effective against Sm.

Conclusion: Sr-derived Se-NPs are effective antibacterial agents against Sm. They're non-toxic, quick, and simple to make, and they could be an inexpensive way to control Sm.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5557

ARROW ROOT MEDIATED SELENIUM NANOPARTICLE AND ITS CHARACTERIZATION | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

The purpose of the research is to synthesize the arrowroot-mediated selenium nanoparticles (SeNPs) and further define their characterization. With its applications in science and technology to create new stuff, the area of nanotechnology is increasingly growing. Today, as the marvel of medicine, nano items are named. Globally, the development of SeNPs is now emerging.

The characterisation of nanoparticles deals with the characterisation of the nanoparticles' physical and chemical properties. Selenium nanoparticles are synthesized and characterized by transmission electron microscopy and UV-vis spectroscopy using Arrowroot. As the shape is rod-shaped and the scale is 200 nm, the effects are observed. Therefore, the nanosized nanoparticles mediated by arrowroot have many applications in modern technology and have high medicinal value as well.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5526

PREPARATION OF SELENIUM NANOPARTICLES USING HERBAL FORMULATION AND ITS ANTIOXIDANT ACTIVITY: AN in vitro STUDY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Background: Compared to different organic and inorganic sources of selenium compounds, selenium nanoparticles (Se NPs) have excellent biocompatibility, bioefficacy, and low toxicity. Se NPs also show positive therapeutic efficacy against various conditions driven by oxidative stress and inflammation.

Goal: The aim of the study was to check the antioxidant activity of the herbal formulations of cloves and cinnamon.

Materials and methods: Cloves (Syzygium aromaticum) and cinnamon (Cinnamomum verum) were powdered and weighed with 5 grammes of powdered cloves and 5 grammes of powdered cinnamon and blended with 100 ml of distilled water. There was a prepared aqueous extract. The extract was then tested using the DPPH assay for antioxidant activity.

Results: Clove and cinnamon extract aqua alcoholic extract showed the highest absorption at a concentration of 10 μl (42.5 percent) when subjected to a DPPH assay to verify its antioxidant function at a wavelength of 355 nm.

Conclusion: It can be inferred that selenium nanoparticles mediated by cloves and cinnamon have the potential to be used as an efficient antioxidant. It can also be employed in large-scale manufacturing and used in many medicinal applications.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5338

ANTI-FUNGAL ACTIVITY OF SELENIUM NANOPARTICLES SYNTHESIZED USING Symplocos racemosa AGAINST Candida albicans | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Background: When left unchecked, Candida albicans emerges as an important cause for morbidity and mortality in debilitated patients. However, with proper inhibitory measures, we can curb its excessive proliferation. Thus, the aim of our study was to examine the plausible antifungal potential of selenium nanoparticles (Se-NPs) synthesized using the bark of Symplocos racemosa (Sr) against Candida albicans (Ca).

Materials and Methods: Sr was powdered and made into a filtered herbal formulation. Sodium selenite solution was added to it and the conjunction was kept in an orbital shaker for the     synthesis of nanoparticles. The Se-NPs were characterized by UV-vis-spectroscopy and were then centrifuged until the final pellet was collected and powdered. A fresh fungal suspension of Ca was dispersed on a Rose Bengal Agar plate and the Agar Well Diffusion method was used. Different concentrations of Se-NPs (50, 100 and 150) μL were incorporated into the wells and were incubated at 37°C for 24 hours. Fluconazole used as a positive control and the zones of inhibition were recorded.

Results: A colour change was observed after the synthesis of Se-NPs. The prepared particles were then characterized by a peak seen at 285 nm in UV-vis-spectroscopy. The zone of inhibition increased in size with an increase in Se-NP concentration. Fluconazole was the most effective against Ca closely followed by 150 μL of our Se-NPs.

Conclusion: Se-NPs synthesized using Sr is an effective antifungal agent against Ca. It is eco-friendly, rapid and easy to synthesize. They are potentially an inexpensive solution for Ca.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5320