In vitro ANTI-INFLAMMATORY ACTIVITY OF Vitis vinifera SEED EXTRACT USING ALBUMIN DENATURATION ASSAY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Introduction: Inflammation is the immune system's primary reaction to harmful stimuli, such as bacteria, infected cells, radioactive substances, or irradiation, and works by eliminating harmful stimuli and initiating the healing process. Like flavonoids, polyphenols, anthocyanins, proanthocyanidins, procyanidins, and the stilbene derivative resveratrol, Vitis Vinifera contains multiple active components. In particular, grape seed extract has been reported to have a wide range of pharmacological and therapeutic effects, such as cardioprotective, hepatoprotective and neuroprotective effects, as well as antioxidant, anti-inflammatory and antimicrobial activities.

Objective: The purpose of the study is to assess the anti-inflammatory activity of grape seed extract using an albumin denaturation assay.

Materials and Methods: First, a concentrated grape seed extract solution was manufactured. This solution was then tested using the Albumin Denaturation Assay at various concentrations (10-50 ug/mL) for its anti inflammatory effect. At 660 nm, the absorbance value was registered.

Results: Grape seed extract has been shown to have strong anti inflammatory activity through this experiment. It has been shown to have stronger anti-inflammatory properties than the sodium-diclofenac norm. It has less side effects than the standard, too. It contains polyphenols, which are responsible for this effect in large part.

Conclusion: The absorbance rate increases at 660 nm with increasing concentrations of grape seed extract, suggesting that the percent inhibition of GSE also increases in the same way.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5533

GRAPE SEED AQUA ALCOHOLIC EXTRACT PREPARATION AND ITS ANTIOXIDANT, ANTIMICROBIAL AND CYTOTOXIC EFFECT - AN in vitro STUDY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Background: Oral disorders, such as dental caries, periodontitis and oral mucosal lesions, are considered to be the most prevalent public health issues worldwide and have a highly important impact on overall health and quality of life.

Objective: To prepare a grape seed aqua-alcoholic extract and analyse its antimicrobial, antioxidant and cytotoxic impact.

Materials and Methods: Red grape seeds were dried and powdered, and water and ethanol were combined with the powdered grape seeds. The aqua alcoholic extract thus obtained was then subjected to a DPPH assay to analyse its antioxidant effect and was loaded into the agar wells developed on Muller Hinton agar in order to test its antimicrobial activity using a well diffusion process. The zone of inhibition was noted against Streptococcus mutans, Lactobacillus and Candida albicans. By using brined shrimp nauplii, the extract was then tested for cytotoxicity.

Results and Discussion: When subjected to a DPPH assay to search for its antioxidant activity at a wavelength of 517 nm, the aqua alcoholic grape seed extract displayed the highest absorbance at a concentration of 50 μl (90.9 percent). The inhibition zone for the prepared extract against Streptococcus mutans was found to be 24 mm, 30 mm and 40 mm at concentrations of 50, 100 and 150 μl, respectively. Similarly, the region of inhibition was found to be 6 mm, 8 mm, and 12 mm for Lactobacillus. The zone inhibition values for Candida albicans were 6 mm, 8 mm and 14 mm, respectively. At 10 μl, the cytotoxicity of the extract was the lowest.

Conclusion: The prepared grape seed extract has proven to be an effective antioxidant and antimicrobial agent and can therefore be used for medical applications. The cytotoxicity assay showed that the extract can be integrated into biomedical applications, thereby concluding that the extract of grape seed can be a groundbreaking step towards the transition to greener medicine.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5349

ANTIOXIDANT PROPERTIES OF GREEN TEA- A REVIEW | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Family of Camellia sinensis (Green tea) is Theaceae. Camellia sinensis is famous because of its more beneficial for health and consist of anti-oxidant, antimicrobial and antioxidant activities and also helps in weight loss. Green tea was admitted by China’s people as powerful customary drink needed for the protection opposed to numerous health issues. The effectiveness of green tea is due to the fact that it contains a number of complex chemicals, which comprises of different classes of chemical compounds, such as amino acids, proteins, alkaloids, minerals, vitamins, polyphenols, etc. So, it is consumed worldwide as it has a number or health benefits. Thus, in this review article, green tea was selected for stressing on its advantages in various medicinal fields and phytoconstituents and recent researches that are done for its quality checking which assures its use in medicinal and pharmaceutical manufacturing. More research is needed to be done to know its other biological activities, active constituents, cheaper and simpler methods for its quality assurance that assures the prevention of its adulteration.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5206