CHARACTERIZATION OF Asparagus racemosus MEDIATED SILVER NANOPARTICLES USING UV - VIS SPECTROPHOTOMETER AND SEM | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Nanoparticles have attracted a lot of interest and have had a major influence on material science. Because of its technological significance, the dominance of nanoparticles will continue in future years with the implementation of incessant research in this sector. The object of the research was to characterize the A. Using a UV Vis spectrometer, racemosus mediates silver nanoparticles. The source of A. Racemosus was obtained as an extract and prepared. For the reduction of Ag+ ions to silver nanoparticles, this extract was used. For the synthesis of nanoparticles, 1 mM of 90 mL silver nitrate solution was prepared and 10 mL of 100 mg plant extract was added and stored in a shaker. The UV visible spectrometer and the scanning electron microscope were used to examine and classify nanoparticles. The Peak appears to confirm the synthesis of nanoparticles using a UV-vis spectrophotometer at 420 nm and the mean size of the nanoparticles has been found to be 150 nm. When used at sufficient concentrations, silver nanoparticles act as a safe alternative to other antimicrobial agents that are chemically derived. Silver nanoparticles may, therefore, be a better alternative agent for the cleaning and disinfection of food industry equipment and surfaces.

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QUALITY CONTROL METHODS OF Acacia catechu EXTRACT BY ATOMIC ABSORPTION SPECTROSCOPIC ANALYSIS | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

The aim of the present study was to evaluate the heavy metal quality control parameters of Acacia catechu bark extract by atomic absorption spectroscopic (AAS) analysis. Acacia catechu is one of the most commonly used herbs in Ayurveda. The whole parts of Acacia catechu tree including stem, bark, leaves, root, and pods are of significant medicinal value in treating many diseases and disorders. The most important actions of Acacia include antioxidant, hepatoprotective, hypotensive, and also as an anti caries, for gingivitis, etc,. Heavy metals such as lead (Pb) and arsenic (As) and minerals such as selenium (Se), chromium (Cr) and potassium (K) are found abundantly in the soil. They are also essential for plant growth and metabolism as micro and macro nutrients. Hence, uptake of these heavy metals and minerals basically occurs in significant proportions in plants. However, these heavy metals and essential trace elements have a tendency to accumulate in alarming proportions in plants leading to toxicity and adverse effects when consumed. Hence, it is important to determine if the heavy metal and mineral content in Acacia is within permissible limits as indicated by WHO. Based on the results of the study, it can be decided whether Acacia catechu can be prescribed for human consumption or not.

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PRIMARY STUDIES ON ANTIMICROBIAL ACTIVITY OF HONDURAN MAHOGANY (Swietenia macrophylla) LEAF EXTRACT

The present study was designed to evaluate the antibacterial activities of Swietenia macrophylla crude methanolic (SMCM) seed extract. The antimicrobial activity of the oily extract against Gram-positive, Gram-negative, yeast and fungus strains was evaluated based on the inhibition zone using disc diffusion assay, minimal inhibition concentration (MIC) and minimal bactericidal concentration (MBC) values. The crude extract was subjected to various phytochemicals analysis. The demonstrated qualitative phytochemical tests exhibited the presences of common phytocompounds including alkaloids, terpenoids, antraquinones, cardiac glycosides, saponins, and volatile oils as major active constituents. The SMCM seed extract had inhibitory effects on the growth of Candida albicans, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and illustrated MIC and MBC values ranging from 25 mg/ml to 50 mg/ml.

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