THE ABILITY OF RHIZOSPHERE FUNGI ISOLATE OF MAHOGANY [Swietenia mahagoni (L.) Jacq.] IN DISSOLVING PHOSPHATE | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 The purpose of this study was to explore the ability of rhizosphere fungus isolates of mahogany stands in dissolving phosphate. Activities and types of microbes which can dissolve phosphate in mahogany plants are essential and needed to be known in order to obtain suitable microbial types to increase plant growth. This study was conducted for two months from March to May 2019 and analyzed in the Laboratory of Biotechnology and Tree Breeding at Faculty of Forestry Universitas Hasanuddin, Makassar. Rhizosphere microbes have important roles in nutrient cycling and during soil formation, plant growth, influencing microbial activity as well as biological control of root pathogens. Preparation of microbial culture, rejuvenation process and preparation of phosphate solubility test media were analyzed with the qualitative method by measuring the absorbance by using a UV-VIS spectrophotometer with 693 nm wavelength. The results of the rejuvenation of fungus isolates that were successfully grown were 27 isolates out of 28 isolates from genus Rhizopus, Fusarium, Aspergillus, Penicillium, Trichoderma and Gliocladium. The highest phosphate concentration was the genus Glioclodium (MB 9.2) with a 6.65 ppm concentration value, while the lowest phosphate concentration was genus Glioclodium (MB 3) with -1.47 ppm concentration value. These isolates produce phosphorus that can be used by plants.

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UNRAVELING THE PLANT GROWTH PROMOTING POTENTIAL OF MICROBES ASSOCIATED WITH Diplazium esculentum AND Clitoria ternatea

 

The main objective to conduct the study was to isolate and characterize the rhizobacteria and unravel their plant growth potential and biological control using various biochemical tests. The study focuses on isolation of Bacillus species related with two himalayan medicinal plant namely Diplazium esculentum and Clitoria ternatea. By cultivation dependent approach 8 bacillus isolates were obtained and further screened for various plant-growth promotion activities. Out of all the isolates only one strain was found to be positive for the production of indole acetic acid. The production of indole acetic acid during plant-microbial interaction is of immence importance because the IAA is a major phytohormone, which controls the plant growth and development. This positive strain will further be characterized based upon 16S phylogeny and will be checked in pots for its plant-growth promotion activity and for the successful development of a commercial bioformulation.

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DIVERSITY OF ENDOMYCORRHIZAL FUNGI IN THE THUYA RHIZOSPHERE, SEFROU REGION (MIDDLE EASTERN ATLAS, MOROCCO)

Tetraclinis articulata is an essence of great socio-economic value. The development of the resistance and survival potential of this species to sometimes extreme environmental conditions are a concern of the managers. for that, the present work is fixed as an objective to evaluate the rate of mycorrhization of thuya roots and to highlight the diversity of arbuscular mycorrhizal fungi (AMF) of thuya from two sites (Beni Souhane and Aderj) of the region of Sefrou (Middle Eastern Atlas). The results obtained showed that the thuya roots carry different structures of endomycorrhizal fungi. The frequencies of mycorrhization of the roots are of the order of 93.3% at the level of site Aderj and 80% at Beni Souhane. The mycorrhizal root intensities are respectively 45.8% and 40% for the Beni Souhane and Aderj sites. Moreover, the arbuscule contents are 26.05% in Beni Souhane and 25.52% in Aderj. The vesicle contents are 14.89% in Aderj and 21.51% in Beni Souhane. The density of endomycorrhizal spores in the rhizosphere of Thuya is 98 and 26 spores / 100 g of soil, respectively in Beni Souhane and Aderj. The identification of isolated spores allowed to note the presence of 37 species belonging to 7 genera, 7 families and 3 orders. The genus Glomus is the most abundant, with a distribution percentage of 63.5%. The species richness is 29 taxa in the site of Beni Souhane and 18 species at the level of Aderj. Besides, the thuya of the Beni Souhane site shows a better rate of mycorrhization of roots and a mycorrhizal species richness greater than that of the Aderj site. This is reflected on the state of forest formation that is coming well to Beni Souhane. It would, therefore, be valuable to develop inocula from the endomycorrhizal fungi present in the Thuya rhizosphere and proceed to controlled mycorrhization in nurseries to obtain resistant and vigorous plants.

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