THE USE OF THREE FUNGAL STRAINS IN PRODUCING OF INDOLE-3-ACETIC ACID AND GIBBERELLLIC ACID | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

The successful conditions of Trichoderma harzianum UzCF-55, Penicillium canescens UzCF-54, Fusarium moniliforme UzGC-12 fungal strains producing Indole-3-acetic acid (IAA) and Gibberellic acid (GA) were investigated in this work. The optimum pH of the nutrients for the strains was found to be 5.5 for Mandel's and 6.8 for the media of Czapek, with incubation temperature intervals of 28-30 ° C for 10 days. Synthesized quantitative study of the IAA by T. Harzianum UzCF-55 showed that the highest acid levels were 1.16 mg/mL and 0.74 mg/mL, respectively, on the sixth day of the exponential growth process of micromycetes, while after the ninth day they were 0.318 mg/ml and 0.17 mg/mL for the GA. During similar days of incubation, the quantities of IAA and GA synthesised by P. canescens UzCF-54 were 0.98 mg/mL and 0.38 mg/mL, both showing higher amounts than the control strain. F. The UzGC-12 moniliforme strain synthesised 0.63 mg/mL of IAA and 0.39 mg/mL of GA, respectively. High-performance liquid chromatography-mass spectrometry (HPLC-MS) studies of micromycetes showed 174.00 m/z mass fractions corresponding to IAA molecular mass, 363.00 m/z of GA7 and 361.00 m/z of GA3, suggesting strain synthesis of gibberellins.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5382

THE ABILITY OF RHIZOSPHERE FUNGI ISOLATE OF MAHOGANY [Swietenia mahagoni (L.) Jacq.] IN DISSOLVING PHOSPHATE | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 The purpose of this study was to explore the ability of rhizosphere fungus isolates of mahogany stands in dissolving phosphate. Activities and types of microbes which can dissolve phosphate in mahogany plants are essential and needed to be known in order to obtain suitable microbial types to increase plant growth. This study was conducted for two months from March to May 2019 and analyzed in the Laboratory of Biotechnology and Tree Breeding at Faculty of Forestry Universitas Hasanuddin, Makassar. Rhizosphere microbes have important roles in nutrient cycling and during soil formation, plant growth, influencing microbial activity as well as biological control of root pathogens. Preparation of microbial culture, rejuvenation process and preparation of phosphate solubility test media were analyzed with the qualitative method by measuring the absorbance by using a UV-VIS spectrophotometer with 693 nm wavelength. The results of the rejuvenation of fungus isolates that were successfully grown were 27 isolates out of 28 isolates from genus Rhizopus, Fusarium, Aspergillus, Penicillium, Trichoderma and Gliocladium. The highest phosphate concentration was the genus Glioclodium (MB 9.2) with a 6.65 ppm concentration value, while the lowest phosphate concentration was genus Glioclodium (MB 3) with -1.47 ppm concentration value. These isolates produce phosphorus that can be used by plants.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5276