PREPARATION AND CHARACTERIZATION OF SILVER NANOPARTICLES BY Justicia adhotoda AND Ocimum sanctum FORMULATION USING TRANSMISSION ELECTRON MICRSCOPY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Biological synthesis requires the reduction of metal ion stabilisation using a number of species. Since plants have a wide range of strong reducing ability, they are an excellent source of silver nanoparticles (Ag Nps). The in vitro synthesis of silver nanoparticles using plant extracts such as Justicia adhatoda and Ocimum sanctum (tulsi) mixture demonstrates that biological synthesis is the most applicable, cost-effective, and environmentally friendly process. The current study describes the biosynthesis of silver nanoparticles in an in vitro setting using plant extracts from Justicia adhatoda and Ocimum sanctum, as well as their characterization using TEM.

The aim of this study is to use TEM to prepare and characterise silver nanoparticles made from Justicia adhotoda and Ocimum sanctum herbal formulations.

Green Synthesis was used to synthesise these nanoparticles, which included using plant extracts from Ocimum sanctum and Justicia adhatoda, followed by characterization using UV-Visible spectroscopy and TEM.

The surface resonance was estimated at 10-35 nm, which is consistent with the formation of silver nanoparticles. The formation of silver nanoparticles was confirmed by TEM images, which showed hexagonal shapes. AgNps come in a range of sizes, including spherical and triangle-shaped nanoparticles with a size of 10-60 nm. The silver nanoparticles were naturally crystalline, with a UV absorbance of about 320 nm.

Conclusion: The antibacterial, antifungal, antioxidant, cytotoxic, and antiinflammatory effects of this formulation can be researched in the future thanks to the TEM characterisation of silver nanoparticles. This innovative formulation may provide new insight into a range of biomedical applications.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5541

OPTIMIZING THE ACCLIMATIZATION PROCESS OF OIL PALM (Elaeis guineensis Jacq.) in vitro PLANTLETS DERIVED FROM THE MATURE ZYGOTIC EMBRYOS | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Oil palm has become one of the most important industrial crops for its use for various purposes, but in vitro regeneration has become a critical route for accelerating breeding programs because of its constraints on traditional propagation. But its low survival rate caused catastrophic losses for the industry during the hardening process. Therefore, we optimized the acclimatization phase for 90-day-old plantlets from mature zygotic embryos germinated in vitro in this research. Using eight soil less media and their combinations in a 1:1:1 ratio, viz., plantlets were acclimatized. HM1 (Vermiculite), HM2 (Cocopeat), HM3 (Perlite), HM4 (Vermiculite + Cocopeat + Soilrite), HM5 (Cocopeat + Perlite + Soilrite), HM6 (Cocopeat + Perlite + Soilrite), HM7 (Cocopeat + Vermiculite + Perlite) and HM8 (Cocopeat + Perlite + Soilrite) (Compost). In terms of plant morphological features such as shoot length, root length, number of leaves, leaf length and leaf width, plantlets grown in the HM4 showed better results after 60 days of growth in the soilless media among all the treatments attempted. But, except for root growth, no statistical significance was observed. However, the introduction of staggered shift to the transition environment significantly decreased the transplantation shock.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5528

In vitro ANTIOXIDANT ACTIVITY OF CHITOSAN NANOPARTICLES AND ITS INCORPORATED LYCOPENE | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Background: Nanotechnology has found its use in several applications including dental diagnosis, materials and therapeutics thus gaining increased popularity in the dental perspectives. Lycopene belongs to the carotenoids that show good pharmacological properties including antioxidant, anti-inflammatory and anticancer. Chitosan may be a biodegradable, biocompatible polymer considered safe for human dietary use and approved for wound dressing applications. In the field of dentistry, chitosan has been used for the prevention of tooth decay as it eliminates bacteria and provides bacteriostatic properties. Hence this study aims to identify the in vitro antioxidant activity of chitosan nanoparticles and its incorporated Lycopene.

Materials and Methods: This In vitro study was done at Saveetha Dental College and Hospitals. The collection and preparation of lycopene extract was done and stored for further use. Synthesis of chitosan nanoparticles was done with the addition of glacial acetic acid along with Sodium Tripolyphosphate (STPP). Centrifugation was done and characterization of chitosan nanoparticles was done using ultraviolet UV-Viz-spectroscopy. The antioxidant activity of chitosan nanoparticles was measured and compared using the standard ascorbic acid assay.

Results: The results of our study show that the antioxidant activity of chitosan nanoparticles and chitosan-lycopene nanocomposite solution has increased activity when compared to the standard ascorbic acid. Hence, these lycopene mediated chitosan nanoparticles have the potential to be used as an effective antioxidant agent.

Conclusion: As the above study showed effective activity of chitosan-lycopene solution, hence it can be used as an effective antioxidant agent for the management of chronic inflammatory diseases. Lycopene loaded chitosan nanoparticles may show a great promise for the development of a drug delivery system by enhancing the cellular accumulation of lycopene with chitosan.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5321