CHARACTERIZATION OF Asparagus racemosus MEDIATED SILVER NANOPARTICLES USING UV - VIS SPECTROPHOTOMETER AND SEM | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Nanoparticles have attracted a lot of interest and have had a major influence on material science. Because of its technological significance, the dominance of nanoparticles will continue in future years with the implementation of incessant research in this sector. The object of the research was to characterize the A. Using a UV Vis spectrometer, racemosus mediates silver nanoparticles. The source of A. Racemosus was obtained as an extract and prepared. For the reduction of Ag+ ions to silver nanoparticles, this extract was used. For the synthesis of nanoparticles, 1 mM of 90 mL silver nitrate solution was prepared and 10 mL of 100 mg plant extract was added and stored in a shaker. The UV visible spectrometer and the scanning electron microscope were used to examine and classify nanoparticles. The Peak appears to confirm the synthesis of nanoparticles using a UV-vis spectrophotometer at 420 nm and the mean size of the nanoparticles has been found to be 150 nm. When used at sufficient concentrations, silver nanoparticles act as a safe alternative to other antimicrobial agents that are chemically derived. Silver nanoparticles may, therefore, be a better alternative agent for the cleaning and disinfection of food industry equipment and surfaces.

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NUTRIENT MEDIA FOR DOUBLE HAPLOID PRODUCTION IN ANTHER CULTURE OF RICE HYBRIDS

The first double haploid lines were obtained in the anther culture more than 50 years ago, but so far their production remains more art than a well-established technology. There are a huge number of articles with conflicting information, both on preliminary processing of anthers and medias that determine the highest yield of regenerants, despite the fact that the relevance of research in this area is difficult to overestimate. Double haploid (DH) lines are used: For increasing efficiency of traditional breeding; obtaining populations of DH lines used in several stages of fixing heterosis effect (to remove semi-lethal genes, as well as homozygotization of material) and so on. The objective of the study is to investigate the efficiency of media for producing DH lines in anther culture of hybrids between Russian and foreign varieties. The anthers of 67 hybrid combinations, both interspecific and intraspecific hybrids, were used. 30-50 anthers were planted on Petri dish (6 сm). After formation of callus (size varied from 2 to 10 mm) on the medium for dedifferentiation, they were transferred to MS medium for regeneration. Plants from 5 cm in size were transferred to the root formation medium. We study the effectiveness of nutrient media, which are derivatives of N6, but with a different hormonal composition; and С, RZ media, the salt content was also changed. Medias RZ and C did not reliably differ in callus yield; the rest were reliably inferior to them in this trait. Therefore, to increase the yield of callus, a higher content of salts of KNO3, KH2PO4, MgSO4 • 7H2O, MnSO4 • 4H2O, ZnSO4 • 7H2O, H3BO3, CaCl2 • 2H2O vitamins is necessary. However, their hormonal composition is different. If the RZ medium contains naphthylacetic (NAA) and dichlorophenoxyacetic acids ( 2,4-D), then C is only 2,4-D. N6-based media, N6a and N6б contain only one auxin as well as C. In the study, the best results were shown by option with content of both hormones at a concentration 2 mg / l NAA and 1 mg/l 2,4-D. The results showed a significant positive effect of increasing iron (FeSO4 x 7 H2O) concentration (55.6 mg / L) both on callusogenesis and plant regeneration. In standard recipe of all the media used in the work, its concentration is 27.8 mg / l. To stimulate somatic embryogenesis, the media were modified also by the addition of abscisic acid (ABA) and silver nitrate AgNO₃. Studies have shown that somatic embryogenesis is stimulated in many genotypes when using ABA in combination with cytokinins. Callusogenesis and regeneration in the considered combinations had maximum values ​​at AgNO₃ concentration 2 mg/l and; 1 mg/l ABA for the of most of the studied hybrids.

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