UTILIZATION OF PINEAPPLE LEAVES FOR PRODUCTION OF NANO CELLULOSE AND ITS APPLICATION ALONG WITH MAGNETIC CHITOSAN FOR EFFICIENT DYE ADSORPTION AND ANTIBACTERIAL ACTIVITY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 The capabilities of a new cellulose – magnetic chitosan composite in terms of dye-adsorption and anti-bacterial action were explained in this report. Absorption spectroscopy, X-ray diffraction, absorption spectrum using Fourier transform infrared spectroscopy, and scanning electron microscopy were used to discern the prepared composites. Methylene blue (MB) was used as a model contamination in dye adsorption experiments. The sorption of the composite was investigated both actively and thermodynamically in order to aid in the removal of Methylene Blue (MB) from aqueous solution. The function of CT (Contact time) is analysed with initial values of temperature and concentration during the filtering of MB out of the aqueous-solution on the composite to check the impact of certain features. The Langmuir-adsorption model was also used to evaluate the thermodynamic parameters ▵H^o, ▵G^o, and ▵S^o. Negative-Gibbs free energy (▵G^o) signals the phenomenon of "adsorption with spontaneity," and the property of positive - adsorption enthalpy (▵H^o) indicates that the procedure is endothermic for the MB. The composite's antibacterial properties were tested against Gram-positive and Gram-negative bacteria, with minimal inhibitory concentrations (MICs) of 0.1 g/mL. This research shows that prepared composite has the ability to act as a green adsorbent as well as an anti-microbial agent, and it offers a one-of-a-kind advantageous role.

Please see the link:- https://www.ikprress.org/index.php/PCBMB/article/view/5609

THE EFFECT OF LIGNIN CONTENT ON LIGNOCELLULOLYTIC ENZYME PRODUCTION USING Trichoderma reesei WITH BMR Sorghum VARIETIES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Current research focuses on the ability to manufacture solid state fermentation cellulase (CMCase, Fpase and β-glucosidase) and xylanase using Trichoderma reesei. Low lignin biomass content was preferred for fast digestibility, so we used Brown Mid Rib (BMR) 22, 24 and Non-Brown Mid Rib CSH 22 low lignin Sorghum varieties as a substrate and were subjected to two pretreatment methods (acid and alkali) to achieve maximum cellulose yield and delignification and to use maximum cellulose yield conditions for further fermentation. The highest CMCase activity (39.42 ± 4.75 IU/g), Fpase activity (13.6 ± 1.82 IU/g) and β-glucosidase activity (2.6 ± 0.19 IU/g) were obtained as substrate using pretreated BMR24 (6% NaOH 100 °C 30 min). However, the highest xylanase activity was observed at 80.9 ± 7.66 IU/g on the 7th day of incubation with pretreated (3% H2SO4 80 °C 30 min) CSH 22 Sorghum biomass as substrate, but BMR 22 and 24 mutant varieties were not active in producing xylanase. The results showed that pretreatment of sorghum biomass BMR 22, 24 and CSH22 effectively reduced lignin content and increased sugar supply during solid state fermentation.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5374