EFFECT OF AUXINS (IAA, IBA AND NAA) ON CLONAL PROPAGATION OF Solanum procumbens STEM CUTTINGS | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Solanum procumbens Lour. is a precious medicinal plant with plenty applications in medicine and pharmacy. Auxin is a plant hormone which is critical to the induction and growth of roots of cuttings. In this study, an experiment was conducted to evaluate the effects of indole acetic acid (IAA), indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA) on the rooting and sprouting of S. procumbens stem cuttings. The experiment involved four distinctive concentrations of IAA (500, 1000, 1500 and 2000 ppm), IBA (500, 1000, 1500 and 2000 ppm) and IBA + NAA (250 + 250, 500 + 500, 750 + 750 and 1000 + 1000 ppm) along with the control group used to treat S. procumbens cuttings. The experiment applied the randomized complete block (RCB) method with 3 replications. The results showed that IAA, IBA and NAA had a remarkable influence on rooting and sprouting abilities of S. procumbens cuttings. The best records of sprouting rate (92.34%), root number (32.25), root length (6.8 cm), root weight (0.574 g), sprout length (11.7 cm) and leaf-pair number (7.5) were obtained in cuttings treated with IBA at 500 ppm. Furthermore, the beneficial effects of auxins wore off as their concentrations increased. In general, this study emphasizes that IBA at 500 ppm is the most effective auxin in terms of stimulating rooting and sprouting in S. procumbens cuttings. The results of the study also promise to create a supply of high-quality S. procumbens seedlings for gardeners.

Please see the link:- https://www.ikprress.org/index.php/PCBMB/article/view/5599

EFFECT OF PLANT GROWTH REGULATORS ON In vitro REGENERATION OF FOUR BANGLADESHI TOMATO (Solanum lycopersicum L.) VARIETIES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

The study was carried out to determine the effect of plant growth regulators (PGRs) on four Bangladeshi tomato varieties on in vitro regeneration. For successful regeneration by callus induction, three explants (cotyledon, hypocotyl, and leaf segments) from four tomato varieties named-BT-2 (BARI Tomato-2), BH-T4 (BARI Hybrid Tomato-4), Apple Tomato, and Native Tomato were used. For callus induction and subsequent plantlet production, MS medium fortified with varying concentrations and PGRs combinations were used. Based on varieties, forms of explants, and hormonal combinations, the nature and texture of the callus were different. In MS+1.0 mg/L BAP + 1.0 mg/L NAA for leaf segment and cotyledon derived BH-T4 and Apple tomato variety explants, the finest callus induction was reported. Full shoot regeneration was achieved with 1.0 mg/L BAP+0.5 mg/L NAA and 0.5 mg/L NAA+1.0 mg/L IAA hormonal combinations with the highest root induction. Well rooted, regenerated plantlets were eventually acclimatized and 70 percent was the best survival rate after acclimatization. Among the four varieties, the most sensitive genotypes were found to be the BH-T4 and apple tomato varieties in terms of different parameters such as primary culture establishment capacity, callus induction rate, shoot initiation, and multiplication, root formation, and acclimatization. This study will help to grow a large number of tomato seedlings per year.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5522