Chrysanthemum indicum MEDIATED SELENIUM NANOPARTICLES AND ITS ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY ABSTRACT | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Introduction: Biological nanoparticle synthesis has risen in popularity in recent years due to its cost-effectiveness, abundant raw material sources, low toxicity, and pharmacological potential. The ability to synthesise SeNPs using bacteria and plants indicates that they may be useful in a number of fields. The aerial sections (stems, leaves, and flowers) of Chrysanthemum indicum have been used in Oriental herbal medicine to treat vertigo, hypertensive symptoms, and a number of infectious diseases.

Materials and Methods: Distilled water was mixed with 1 mL of Chrysanthemum indicum extract. The mixture was heated for 15 to 20 minutes at 60 degrees Celsius before being filtered. For the synthesis of nanoparticles, 0.519 grammes of Selenium were applied to the filtered extract and alternated between an orbital shaker and a magnetic stirrer. To assess the antioxidant activity of Chrysanthemum indicum mediated selenium nanoparticles, a DPPH radical scavenging assay was used. The anti-inflammatory activity of Chrysanthemum indicum mediated selenium nanoparticles was assessed using an albumin denaturation inhibition assay. The reagent for the assay was BSA (Bovine serum albumin).

Results: The antioxidant and anti-inflammatory activity of five different concentrations of reaction mixture were tested in this study: 10 L, 20 L, 30 L, 40 L, and 50 L. Plant extract induced by selenium nanoparticles at a concentration of 50 litres had the highest antioxidant activity and anti-inflammatory activity, both of which were inhibited by 87 percent.

Conclusion: In both assays, the antioxidant and anti-inflammatory activity of Chrysanthemum indicum mediated selenium nanoparticles was effective.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5619

GREEN SYNTHESIS OF Solanum trilobatum MEDIATED SELENIUM NANOPARTICLES AND ITS ANTI-INFLAMMATORY AND ANTI-MICROBIAL ACTIVITY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Goal: The aim of the present study is to assess the anti-inflammatory and anti-microbial activity of selenium nanoparticles mediated by Solanum trilobatum.

Materials and Methods: 1g of Solanum trilobatum powder with 0.519 g of sodium selenite with 50 ml of distilled water was used to prepare the plant extract. They then collected the extract and held it in a laboratory shaker for a few hours to synthesise selenium nanoparticles. The colour shift was detected every two hours and the readings were taken using a UV spectrometer.

Results and Discussion: Selenium nanoparticles showed a higher percentage of protein denaturation inhibition at 50 μL due to the anti-inflammatory impact. If the concentration increases, the inhibition percentage simultaneously increases. Effective inhibition against Streptococcus mutans, Lactobacillus sp and Candida albicans was shown by the anti-microbial activity of Solanum trilobatum mediated selenium nanoparticles. In our research, antimicrobial activity showed a higher-than-standard inhibition zone against S. Lactobacillus and mutans. In our research, antifungal activity showed an inhibition zone against Candida albicans almost identical to the norm used.

Conclusion: Due to the altered and outstanding properties of nanoscale materials, nanoscience and nanotechnology have numerous promising applications. The current study has shown that SeNPs controlled by Solanum trilobatum have excellent anti-inflammatory and antimicrobial activity. So this present study will provide understanding and pave a way for new medicines to be developed that could replace the norm in the future.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5311