Objective and Context: Brown algae contain a number of chemical components
(carotenoids, laminarin, alginate, fucoidan, mannitol and phlorotannin) that
are used to treat various degenerative diseases as anti-cancer agents,
antioxidants and chemo-preventive agents. One of the algae species belonging to
the Phaeophyceae class, or brown algae, is Sargassum polycystum. Content of
phenols in S. Polycystum may function as an anti-bacterial pathogen,
particularly E. S. and coli. Bacteria with aureus. Therefore, the purpose of
this study was to determine the total phenol and its antibacterial activity
from the semi-polar to the polar extract.
Materials and methods: Maceration with 96 percent ethanol, acetone and ethyl
acetate as the solvent was used in the extraction process. The total phenol
content was measured using the foline ciocalteau reagent with a gallic acid
standard UV VIS spectrophotometer. While the extract's antibacterial activity
was performed with gram-negative E using the disc diffusion zone process.
Gram-positive S and coli. Bacteria with aureus.
Results and Discussion: The results showed that the S. Phenol with a gross
phenol content of 96 percent ethanol 3.75 mg EGA/g, ethyl acetate 5.78 mg EGA/g
and acetone 4.04 mg EGA/g were found in polycystum extract. The 96 percent
antibacterial activity of ethanol, ethyl acetate and acetone extracts was rated
as high with a range of 10-13 mm inhibition zone diameter. The highest overall
phenol content and antibacterial activity occurred with ethyl acetate extract.
Conclusion: The conclusion of this analysis is that ethyl acetate with the
maximum total phenol content of 5.78 mg EGA/g is the most effective solvent for
phenol attraction. This is a positive association with the findings of the
ethyl acetate extract's antibacterial activity, which has a higher inhibition
zone activity relative to other E extracts. S. and coli. Bacteria with aureus.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5466
Showing posts with label total phenol. Show all posts
Showing posts with label total phenol. Show all posts
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