ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF Acalypha indica MEDIATED SELENIUM NANOPARTICLES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Nanoparticles are a fascinating field because they cross the gap between bulk materials and atomic structures. Nanoparticles may be made from a number of materials, including proteins, polysaccharides, and synthetic polymers, and the materials used are determined by the nanoparticle size, biocompatibility, and toxicity. Selenium nanoparticles have gotten a lot of attention because of their beneficial properties and bioactivities, such as lower toxicity than inorganic and organic forms, and they've become a popular option in nanoparticle research. Acalypha indica is a tropical annual herb with erect, sometimes simple-stemmed stems that has been recognised by locals as a valuable source of medicine for a range of therapeutic treatments. It has anti-inflammatory, antibacterial, and cancer-fighting properties. Selenium nanoparticles, on the other hand, were appealing due to their properties.

Aim: To see whether selenium nanoparticles produced by A. indica have antimicrobial and antioxidant properties.

Methodology: Antioxidant Profile by DPPH activity and Well diffusion method for antimicrobial activity were performed after the preparation of A.indica mediated Selenium nanoparticles.

A. indica mediated the results. Se nanoparticles have strong antioxidant and antimicrobial properties.

Conclusion: At high concentrations, Acalypha indica-mediated selenium nanoparticles have strong antimicrobial and antioxidant properties.

Please see the link:- https://www.ikprress.org/index.php/PCBMB/article/view/5608

CYTOTOXIC AND ANTIOXIDANT POTENTIAL OF Hybanthus enneaspermus MEDIATED SILVER NANOPARTICLE | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Aim: The aim of the study is to synthesis Hybanthus enneaspermus mediated silver nanoparticles and to evaluate the cytotoxic and antioxidant potential.

Introduction: Hybanthus enneaspermus (F. Muell) belongs to the family Violaceae. The plant Hybanthus enneaspermus is reported to have antimicrobial, anticonvulsant, nephroprotective, hepatoprotective, anti-inflammatory, aphrodisiac, aldose reductase inhibitory and free radical scavenging activities. Ag NPs produce reactive oxygen species and free radicals causing apoptosis (cell death) thereby preventing their replication. Many researchers have correlated the plants antioxidant potential with their phenolic constituents. Antioxidants have resistance against oxidative stress by scavenging free radicals, thereby preventing many diseases.

Materials and Methods: Cytotoxic activity: Brine shrimp eggs were obtained from the new aqua laboratory. Filtered artificial seawater was prepared, then shrimp eggs were added into the dark side of the chamber while there was a lamp above the other side to attract the hatched shrimp. Two days were allowed for shrimp to mature, after two days the shrimp Larva was ready. 10 brine shrimps were added accordingly to the silver nanoparticle. It added 5, 10, 15, 20 micrometer and it was left for 24 hrs and observation was made.

Antioxidant activity: 50% methanol, DPPH solution and Hybanthus enneaspermus mediated silver nanoparticles were added in 5 test tubes ranging from 10-50 microliters and kept in a dark place for 10 minutes and the reading was recorded using photometry.

Results and Discussion: In the cytotoxic activity as the concentration of the silver nanoparticle increases the percentage of lethality of brine shrimps also increases. In the antioxidant activity as the concentrations of silver nanoparticles increases the percentage of inhibition also increases.

Conclusion: With the help of the study we can conclude that silver nanoparticles of Hybanthus enneaspermus possess both cytotoxic and antioxidant activity.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5359