Friday, January 31, 2020

PLANT PRIMARY METABOLITES

Plants are the primary food producers and they comprise the essential wellspring of carbon, nutrients, minerals, protein and unsaturated fats. Plant proteins can help in anticipation of cardiovascular illness, disease, bone misfortune and diabetes. Cereal grains, legumes including oil-seed provide proteins to the entire animal kingdom. Plant enzymes like proteases, amylases, cellulose and lipase are useful in the digestion of raw food. Plants have been used to treat many ailments from time immemorial. Moreover, much work has been done to study the therapeutic effect of secondary metabolites from plants rather than the primary metabolites namely proteins, carbohydrates, enzymes and lipids. This review stresses upon the use of plant proteins and enzymes as a whole for treating diseases/disorders rather than the secondary metabolites.

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Thursday, January 30, 2020

GENETIC VARIABILITY AND HERITABILITY STUDIES IN Crossandra infundibuliformis (L.) Nees

Investigation to find out the amount of variability present in the Crossandra [Crossandra infundibuliformis (L.) Nees] was carried out at Horticultural College and Research Institute, Periyakulam during 2016-17 using local Nilakottai genotype. The mutagens employed were gamma rays at different estimates of phenotypic variance (phenotypic variation) were higher compared to the genotypic variance (genotypic variation) for all growth and flower characters, indicating the influence of environment. Among the morphological characters studied, high (phenotypic and genotypic variation) were recorded for a number of flowers per plant. Whereas all the other traits showed moderate to low values. All the traits recorded very high heritability in M2 generation in mutant populations of Nilakottai local. The genetic advance as per cent of the mean was high for plant height, number of leaves, internodal length, number of branches, length of the branch, number of days for first flower to emerge, number of spikes per plant, number of flowers per spike, number of flowers per plant, length of spike, yield of flowers per plant, weight of 100 flowers. High heritability values recorded for all the traits measured indicates that genetic variation can be created using Nilakottai local thus ensure continuous improvement through breeding of Crossandra plant.

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Tuesday, January 28, 2020

ADSORPTION OF DYES BY MARINE ALGAE

Adsorption is the adherence to a surface of atoms, ions, or molecules from a gas, liquid, or dissolved solid. This method produces an adsorbent film on the adsorbent's ground. In the present study, Ulva intestinalis (formerly Enteromorpha intestinalis) a member of Cyanophyceae collected form Covelong, Southeast coast of Tamil Nadu was used for adsorbing methyl orange and phenol red. The results showed that at 60 min of treating the phenol red dye with seaweed yield maximum reduction (44%) of colour and in case of methyl orange 47.4% colour reduction occurred after 60 min.

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Monday, January 27, 2020

A REVIEW ON ANTIMICROBIAL ACTIVITY OF VARIOUS Avicennia SPECIES

Mangrove crops were used in drugs for folklore. Mangroves are woody trees and shrubs that develop in tropical and subtropical intertidal areas. A. marina, frequently referred to as gray mangrove or white mangrove, is a mangrove species categorized as Acanthaceae (formerly Verbenaceae or Avicenniaceae) crop genus. It happens in the intertidal regions of estuarine areas, as with other mangroves. The A. marina samples have more effective antioxidant activity. This survey report views on various antimicrobial and phytochemical activities of Avicennia species.

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Friday, January 24, 2020

EFFECTS OF VARYING LEVELS OF ALUMINIUM ON GERMINATION OF RADISH (Raphanus sativus), CHICKPEA (Cicer arietinum) AND MUNG BEAN (Vigna radiata)

Overwhelming metals are one of the most significant natural poisons. An examination was done to discover the impact of aluminium in hydroponics culture on the germination of Raphanus sativus, Vigna radiata and Cicer arietinum. The plants were permitted to develop in fixed hydroponic culture at different groupings of aluminium chloride at various concentrations (0, 5, 10, 15, 20 and 25 mM). It was discovered that there was abatement in germination rate with an expansion in the centralization of the aluminium. Raphanus sativus and Vigna radiata seeds were able to withstand an aluminium concentration of 10 mM and 25 mM, respectively while Cicer arietinum was very sensitive to Al and could withstand only up to 5 mM of aluminium.

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EFFECT OF TIME OF MIXING ON DYE REMOVAL CAPACITY FROM WASTEWATER

Nowadays finding the Easy and Economical way to treat the industrial wastewater is the raising ideas in newer technology. In this work adsorption of methylene blue using various adsorbent such as groundnut shell, and tea waste were used in batch studies at a constant dye concentration of 300 mg/l, adsorbent dose of 0.5 gm groundnut shell powder, and Spent tea powder. The time of Agitation was varied by 20, 40, 60, 80, 100 min. After the time of agitation of 100 min, Maximum dye removal of 86% was observed in spent tea powder and 80% was observed in Groundnut shell powder.

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COMBINED EXPERIMENTAL AND COMPUTATIONAL APPROACHES FOR CHEMICAL-KINETICS INVESTIGATION OF BENZOYL ISOTHIOCYANATE FORMATION

Kinetics and mechanism of the reaction between substituted benzoyl chlorides (1) and ammonium thiocyanate (2) were investigated theoretically and experimentally using the DFT method at M062x/6‐311G++(2d,2p) level of theory and UV-vis spectrophotometry technique, respectively. The reaction followed second‐order kinetics according to the effect of concentration on the reaction rate. The solvent effect demonstrates that media with the lower dielectric constant is in favour of the reaction rate. On the basis of  the Eyring plot, activation parameters were determined in a lower dielectric constant solvent such as 1,4 dioxane, the low value of ∆G (58.7 kJ mol-1) in this solvent relation to polar solvent, help to increase the reaction rate. In fact, unfavourable ∆S value (-188.18 J mol-1 K-1) can be compensated by the favourable ∆H value (lower, 4.01 kJ mol-1). In this case, the reaction is entropy controlled, while in the polar solvent (acetonitrile), the unfavourable ∆H value (higher, 45.6 kJ mol-1) can be compensated by the favourable ∆S value (-80.9 J mol-1 K-1), so the reaction is enthalpy-controlled. Different substituents examined on the reaction rate in both methods. The rate constant was in favour of strong para electron-withdrawing substituent (EWS) groups (i.e. NO2) on benzoyl chloride. A comparison of theoretical and experimental rate constant values in both methods indicated differences between data. This is expected, because of the real liquid phase (for experimental results) has a great difference from the unlike liquid phase (for theoretical data). Hammet study, exhibited that the large value of ρ=1.94 imply that TS structure is constructed with negative charges; hence, EWS plays a significant role in stabilizing TS character for increasing the reaction rate. The result of this study confirmed that the reactions in the presence of various para-substituted benzoyl chlorides have the same kinetics role. Also, the effect of leaving group was studied on the reaction between (1) and (2), theoretically. The result showed that the reaction rate in the presence of benzoyl bromide has been increased approximately 25 times more in the gas phase and also 170 times more in a liquid phase, compared to benzoyl chloride. A linear dependence of ΔH versus ΔS approved based on the isokinetic and Exner equations, so the reaction exhibited the same kinetics role in the different solvents.

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Thursday, January 23, 2020

EVALUATION OF GERMPLASM FOR VARIABILITY IN BREEDING AND SEED YIELD TRAITS IN Brassica juncea (L)

The present study was undertaken to evaluate various Genetic parameters like Variability, Heritability, Genetic advance and Genetic advance percent as mean for twenty-six genotypes of Indian mustard (Brassica juncea) L. by using RBD design in Rabi 2018 – 2019 at Experimental farm of the lovely professional university, Punjab. High phenotypic and genotypic coefficient variation (PCV and GCV) was reported in number of secondary branches (38.98 and 33.82), harvest index (35.58 and 25.49), biological yield (35.40 and 30.24), seed yield per plant (31.02 and 29.18) and number of primary (24.04 and 20.83), High magnitude heritability was recorded in all most all characters except harvest index and seeds per silique. High genetic advance recorded for plant height (42.06), length of the main shoot (42.35) and biological yield (45.47), genetic advance percent mean recorded high recorded for all most all traits except days to first flower, whereas selection of above traits would help in the effective improvement on seed yield per plant.

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IMPACT OF MINERAL FERTILIZERS ON LEAF MINERAL CONTENT (CV NENDRAN) AND NUTRIENT STATUS OF THE STUDIED SOIL

Nendran is the popular variety grown commercially in different regions due to its wider adaptability and high degree of tolerance to drought in a perennial cropping system. A field experiment was performed in randomized block design with ten treatments of various ratios of nitrogen and potassium to study their nutrient concentrations in banana leaves and soil at vegetative and harvesting stage. The results revealed that the treatment 9 with the ratio of N250P80K400 showed the highest mineral content in leaves at both the vegetative and harvesting stage. Therefore the fertilizers with the dosage of N250P80K400 are optimum for growing the plants.

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PRIMARY STUDIES ON ANTIMICROBIAL ACTIVITY OF HONDURAN MAHOGANY (Swietenia macrophylla) LEAF EXTRACT

The present study was designed to evaluate the antibacterial activities of Swietenia macrophylla crude methanolic (SMCM) seed extract. The antimicrobial activity of the oily extract against Gram-positive, Gram-negative, yeast and fungus strains was evaluated based on the inhibition zone using disc diffusion assay, minimal inhibition concentration (MIC) and minimal bactericidal concentration (MBC) values. The crude extract was subjected to various phytochemicals analysis. The demonstrated qualitative phytochemical tests exhibited the presences of common phytocompounds including alkaloids, terpenoids, antraquinones, cardiac glycosides, saponins, and volatile oils as major active constituents. The SMCM seed extract had inhibitory effects on the growth of Candida albicans, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and illustrated MIC and MBC values ranging from 25 mg/ml to 50 mg/ml.

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Wednesday, January 22, 2020

PRODUCTION AND OPTIMIZATION OF SUCRASE FROM Bacillus subtilis USING PLACKETT BURMANN DESIGN

Over the few years, there is an increased interest in the microbial enzymes to overcome its inability to meet the current and future requirements of the World. In the search of a kind, sucrase has a vital role to play with its variety of applications, particularly in the food and biofuel productions. In addition to their vast and varied applications, newer microbes are to be screened for sucrase production with their desirable properties. There are two saccharolytic enzymes induced by sucrose in Bacillus species which is encoded by SacB and SacA in the an extracellular levansucrase and an intracellular, respectively. In this experimental analysis, Plackett Burman Design was used for screening of nutrients for sucrase production by Bacillus subtilis. Sucrase activity was optimized by Plackett Burman design in Production Medium and then purified by column chromatography. Using MINITAB 15 Software, Sucrose, Yeast Extract, and Ferrous Sulphate had major source influence on sucrase activity compared to other components. In column purification, maximum amount of enzymes was obtained from the concentration of 0.5M NaCl-eluted sample.

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Tuesday, January 21, 2020

ON THE BOUNDED AND UNIQUE SOLVABILITY OF THE BOUNDARY VALUE PROBLEM OF THE EQUATION IN THE SPACE OF SCALAR FUNCTIONS WITH ABSOLUTE CONTINUOUS DERIVATIVE OF THE (n -1) ORDER AND ITS ISOTONIC GREEN OPERATOR FOR A CERTAIN CLASS OF LINEAR FUNCTIONAL DIFFERENTIAL EQUATIONS

The objectives of this paper is to investigate the boundary value problem of the equation in the space of scalar functions with absolute continuous derivative of the nth order, and to establishes the effective and sufficient conditions for its bounded and it unique Solvability. Theorems were stated and prove under the Preliminaries note, about four Theorems with applications to prove the main results. The necessary and sufficient conditions that guarantee the studied boundary value problem to satisfy the Isotonic Property of the Green Operator was also established. My approach in this study improved on the literatures, to the case where more than two arguments of the studying equations were established, as in the case of one argument in the authors in [3,4].

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Friday, January 17, 2020

NUTRIENT MEDIA FOR DOUBLE HAPLOID PRODUCTION IN ANTHER CULTURE OF RICE HYBRIDS

The first double haploid lines were obtained in the anther culture more than 50 years ago, but so far their production remains more art than a well-established technology. There are a huge number of articles with conflicting information, both on preliminary processing of anthers and medias that determine the highest yield of regenerants, despite the fact that the relevance of research in this area is difficult to overestimate. Double haploid (DH) lines are used: For increasing efficiency of traditional breeding; obtaining populations of DH lines used in several stages of fixing heterosis effect (to remove semi-lethal genes, as well as homozygotization of material) and so on. The objective of the study is to investigate the efficiency of media for producing DH lines in anther culture of hybrids between Russian and foreign varieties. The anthers of 67 hybrid combinations, both interspecific and intraspecific hybrids, were used. 30-50 anthers were planted on Petri dish (6 сm). After formation of callus (size varied from 2 to 10 mm) on the medium for dedifferentiation, they were transferred to MS medium for regeneration. Plants from 5 cm in size were transferred to the root formation medium. We study the effectiveness of nutrient media, which are derivatives of N6, but with a different hormonal composition; and С, RZ media, the salt content was also changed. Medias RZ and C did not reliably differ in callus yield; the rest were reliably inferior to them in this trait. Therefore, to increase the yield of callus, a higher content of salts of KNO3, KH2PO4, MgSO4 • 7H2O, MnSO4 • 4H2O, ZnSO4 • 7H2O, H3BO3, CaCl2 • 2H2O vitamins is necessary. However, their hormonal composition is different. If the RZ medium contains naphthylacetic (NAA) and dichlorophenoxyacetic acids ( 2,4-D), then C is only 2,4-D. N6-based media, N6a and N6б contain only one auxin as well as C. In the study, the best results were shown by option with content of both hormones at a concentration 2 mg / l NAA and 1 mg/l 2,4-D. The results showed a significant positive effect of increasing iron (FeSO4 x 7 H2O) concentration (55.6 mg / L) both on callusogenesis and plant regeneration. In standard recipe of all the media used in the work, its concentration is 27.8 mg / l. To stimulate somatic embryogenesis, the media were modified also by the addition of abscisic acid (ABA) and silver nitrate AgNO3. Studies have shown that somatic embryogenesis is stimulated in many genotypes when using ABA in combination with cytokinins. Callusogenesis and regeneration in the considered combinations had maximum values ​​at AgNO3 concentration 2 mg/l and; 1 mg/l ABA for the of most of the studied hybrids.

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THE ROLE OF ADULT EDUCATION IN THE PROMOTION OF PARTICIPATORY AND REGIONALISED DEVELOPMENT FOR RE-CHARACTERISATION OF SADC

The development of the Southern African Development Community (SADC) remains pivotal for transformation and amelioration of the living conditions of the citizens of the member states. It is therefore essential for countries that are members of SADC to realise economic prosperity, political liberalisation, social transformation and prudent utilisation of environmental finite resources through the active participation of citizens of the member states. The efforts that are geared towards the realisation of a prosperous SADC should be employed through the promotion of regionalised policies and re-characterisation of the citizenship agenda. SADC economic, political, social and technological interventions are marred with ostracising of the majority of the people in the regional bloc. The ostracising of some regional citizens is based on race, tribe, political affiliation, sexual orientation, socio-economic status and ideological orientation, which result in them been denied the opportunities to participate in the activities that are meant to transform their lives. The research presents some discourse on the role of adult education in sensitising and educating the ordinary people within the SADC countries, to generate narratives around the issues that lessen their power to participate actively in the efforts that are towards the building of the regional body, which is supposed to have a clear agenda for participatory development. It is noted that the SADC countries engage in various activities that are apt for their development, amidst the discriminatory characteristics that leave a significant number of the underprivileged and those with divergent views with no room to participate. The influence of the corporate entities has rendered the states in the SADC region less accommodative of indigenous ideas, with preference been given to exotic ideologies by the governing elites. The paper exemplifies this position by showing how Basarwa in countries such as Botswana, Namibia and South Africa do not play any noticeable economic, political, social and technological roles in the running of their respective countries. It is argued therefore that SADC should through Adult Education promote the necessary camaraderie and quid pro quo within the citizens of the SADC countries so that their knowledge of what obtains in their respective countries can be achieved. If the regional body is to be more responsive, it has to be more accommodative and it is through educating its constituents that it can realise both qualitative and quantitative forms of development. The paper argues that adult education is chiefly important for any form of development to take place because it is a vehicle through which underprivileged sections of the populations such as the San (Basarwa) can be transformed, so as to be knowledgeable, skilful and have germane attitudes to foster their participation and enhance regional development.

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Thursday, January 16, 2020

KINETICS AND THERMODYNAMICS APPROACH FOR IMMOBILIZATION OF ALPHA-AMYLASE AND MALTOGENIC AMYLASE BY NANOMAGNETIC COMBINED CROSS-LINKED ENZYME AGGREGATES METHOD FOR STARCH CONVERSION TO MALTOSE SYRUP

The thermostability enhancement of specified nanomagnetic combi-CLEAs of α-amylase and maltogenic amylase was evaluated by assessment of kinetic parameters, temperature dependence, enzyme half-life, thermal inactivation energy barriers (Ea(in)), and thermodynamic parameters in a storage thermostability approach at 55–95° C for 120 minutes. The Michaelis constant (Km) values of NM-Combi-CLEAs was diminished within 1.5 folds while The catalytic efficiency was enhanced near 1.32 after CLEAs formation. The half-lives of the fabricated nano-bio catalyst increased about 2.5 folds at 95º C. Besides, Ea(in) of the native combined enzymes was appended from 44.75 into 54.76 (KJ.mol-1) and ΔH* increased from 41.69 to 51.70 (KJ.mol-1) while, ΔS* decreased 28.2% after immobilization. The NM-Combi-CLEAs conserved 80.4% of its original activity after ten cycles. Hence, the advanced technique for NM-Combi-CLEAs production is introduced as an effective and secured method to fabrication of recyclable immobilized enzyme also, resolving of mass transfer limitation difficulties and ease in enzyme handling.

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Saturday, January 11, 2020

ISOLATION AND ANTIBIOGRAM OF PATHOGENIC BACTERIA (Escherichia coli and Staphylococcus sp.) FROM DIFFERENT KINDS OF CHEESE AVAILABLE IN MARKET OF CHATTOGRAM IN BANGLADESH

Cheese is a solid, fresh or ripened product obtained by adding the enzyme rennet in the presence of lactic acid produced by the adventitious microorganisms that causes coagulation and whey separation of milk, cream or partly skimmed milk, buttermilk. Cheeses of different types especially soft and semi-soft are suspected to the presence of pathogenic E. coli and Staphylococcus sp. which are harmful for public health. The purpose of the current research was to investigate the qualities of cheese collected from super-shops of Chattogram. A total of 15 cheese samples were collected from three super-shops of Chattogram. Among 15 cheese samples, the S3 sample collected from shop-B had the highest bacterial load (1.80×107 cfu/ml) than those in shop-A and shop-C. Isolation of pathogenic E. coli, Staphylococcus sp. was done by using selective media. Four out of the total 15 cheese samples were found to be contaminated with E. coli (26.67%) while ten were detected as contaminated with Staphylococcus sp. (67%). The antibiotic susceptibility test was done to detect susceptibility to selective antibiotics. E. coli and Staphylococcus sp. showed almost 100% resistant behavior to ampicillin, amoxycilin, tetracycline and 50% sensitivity of E. coli to gentamicin and ciprofloxacin while for Staphylococcus sp. sensitivity to gentamicin, ceftriaxone, ciprofloxacin was 27.27%, 90.91%, 18.18% respectively. The screening for shiga-toxin strains in E. coli isolates was based on the detection of the two genes stx1 & stx2 and tetracycline resistant strains of E. coli were detected for Tet A, Tet B & Tet C genes by polymerase chain reaction (PCR). The screening for methicillin resistant in Staphylococcus sp. isolates was based on the detection of the mecA gene by polymerase chain reaction (PCR). In conclusion, the risk of pathogenic bacteria is higher in cheese if safety measures not maintained & could pose a massive health risk.

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SELF-EFFICACY AND IT’S INFLUENCE ON THE ACADEMIC PERFORMANCE OF THE SENIOR HIGH SCHOOL STUDENTS

This study investigated the influence of self-efficacy on the academic performance of the Grade 12 Senior High School students at the St. Paul College of Bocaue. The findings of the study concluded that the level of the self-efficacy of the respondents was high. Likewise, the findings suggested that the students are resilient and determined in the face of setbacks, feel assured that they will be able to learn the classroom discussion and excel on their assessment, and they tend to be more intrinsically motivated to learn new knowledge. Furthermore, the computed linear regression analysis results revealed that self-efficacy does not significantly affect the Senior High School students’ academic performance. This suggests that even if the students have a high level of self-efficacy it doesn’t influence their academic performance in class. However, it doesn’t mean that the school should not give importance to develop the students’ self-efficacy. Hence, it is suggested that the school should continue its academic programs that will continuously develop the student’s well-being in the process of acquiring relevant experiences and skills in the school.

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PURIFICATION AND CHARACTERIZATION OF EXTRACELLULAR LIPASE ENZYME FROM Aspergillus costaricaensis CBS115574

In the present study, the lipase of alkaline nature obtained from the previously isolated strain Aspergillus costaricaensis CBS115574 was characterized and purified out using ammonium sulfate precipitation and chromatographic techniques on Diethylaminoethyl A-50 and Sephadex G-100. The purified lipase has the molecular weight of ~50 kDa which was determined by Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Zymography by using the phenol red along with the purification fold of 6.63 and specific activity of 31.73 IU/mg after the gel filtration chromatography. Lipase was found to be stabilized at the pH 7 (122.68 IU/g/min) and at the temperature of 80ºC (141.36 IU/g/min) with its optimal activity at pH 8 and temperature 50ºC. The Km and Vmax value for extracellular lipase were reported to be 29.62 mM and 74.07 IU/ml with their higher tolerance to glycerol (organic solvent), tween 80 (detergent), Ethylene diamine tetra acetic acid (activator & inhibitor), hydrogen peroxide (oxidizing & reducing agent) and sodium chloride (metal ions) with lipase activity of 106.74 IU/g/min, 84.53 IU/g/min, 108.06 IU/g/min, 97.17 IU/g/min and 118.7 IU/g/min. The above results suggested that A. costaricaensis CBS115574 lipase found a suitable position for application in different types of industries.

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OPTIMIZATION OF CULTURAL PARAMETERS OF Aspergillus costaricaensis CBS115574 FOR LIPASE PRODUCTION BY SOLID-STATE FERMENTATION

In the present study, Aspergillus costaricaensis CBS115574 which was previously isolated from the non-leguminous field soil collected from the Kishangarh city of state Rajasthan confirmed by 16S rRNA gene/ITS/D1-D2 region sequencing. The cultural parameters (Carbon sources, nitrogen sources, moisture content, pH, temperature and particle size etc) of solid-state fermentation have been optimized for the improved extracellular lipase production. Among different carbon sources, A. costaricaensis showed best lipase activity (253.6 IU/g/min) in the presence of lactose. Among the various concentration (0.2%, 0.4%, 0.6%, 0.8%, 1.0%, 1.2%, 1.4% and 1.6%) of lactose, the optimum concentration was found to be 1.6%. Among different nitrogen sources, highest lipase activity (55.32 IU/g/min) was obtained in the presence of yeast extract and the optimum concentration of yeast extract was found to be 0.5% for maximum lipase activity (236.69 IU/g). Further the best lipase activity was obtained in the medium of pH 8.0 (312.330 IU/g/min) at 28ºC after 48 hrs of incubation period. Further the particle size of solid substrate was optimized and highest lipase activity was found when fine particle size was used. Among the various ratio of moisture content, highest lipase activity (317.09 IU/g/min) was found at 1:6 moisture content. From the present work, it is figure out that for the enhanced production of lipases the medium parameters required to be optimized. The above results help out the authors in carry out the further studies of purification and characterization of lipase enzyme produced from A. costaricaensis CBS115574.

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FUZZY INFERENCE MODEL OF CARBON DIOXIDE CONSUMPTION FOR CALCIUM CARBONATES PRODUCTION

Experiments performed of calcium carbonate production by passing carbon dioxide through within calcium hydroxide mixture prepared in deionized water are considered at low to high temperature each at a time. In this paper, a built fuzzy interface model of inputs pH, time and temperature with output reaction conversion is constructed. Considering a constant temperature, fuzzy inference of carbon dioxide consumption is orderly inference. Various membership functions, each at a time, are placed to find representation with the process output. Broader shoulder membership function response is found closing.

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Friday, January 10, 2020

NUMERICAL PRACTICALS OF GAS JETTING INTO WALL BOUNDED SYSTEMS


In this work, considering a jet of air from the aside slit; scope is the air jetting onto the fluids contained within the container; the control volume is constructed. This wall bounded closure is simulated after meshing for various situations and jet path shows several flow zones. For empty system the jet shows turns and returns along the walls which elucidate the bubble formation and air trap positions. When the system is put with fluids compositely, in a half space, the layers are formed which may define the zone rising or flowing down with jet regimes. Gaseous mixture striding waves are observed too. Different levels of mass transfer are identified at interfaces and zones are isolated. These aftermath numerical practicals show the way to explain many operations during processing.

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Monday, January 6, 2020

CHARACTERIZATION AND RP-HPLC METHOD DEVELOPMENT OF A BIOMARKER (BERGENIN) FROM THE ROOTS OF Securinega virosa

The quality of the roots of Securinega virosa (Euphorbiaceae) commonly marketed in Ghana for medicinal purposes needs to be assured through standard quality control procedures. This work has isolated and characterized a biomarker to be used in assessing the quality of products and herbal preparations containing the roots of Securinega virosa. Using column chromatography of 20 g ethanolic roots extract; 0.02% w/w biomarker was obtained and characterized by melting point determination, UV-Vis, IR, 1D and 2D NMR spectroscopic analysis and comparison to reported literature. The biomarker was identified as Bergenin. A RP-HPLC method has been developed and validated for the quality control of S. virosa root preparations with Bergenin as standard. The most appropriate resolution was attained by a gradient elution of methanol: 0.05% v/v trifluoroacetic acid in water using Waters Bondapak C18 (3.9×300 mm, 5 μ) column at a flow rate of 1.0 ml/min and a wavelength of 220 nm. The retention time of the biomarker was found to be 14.20 ± 0.01 mins. The method was subjected to ICH guidelines for validation and showed a good linearity with a correlation coefficient (r) of 0.9993. The LOD and LOQ were found to be 15.23 mg/L and 46.16 mg/L respectively. Tolerable levels of accuracy, precision, specificity and robustness were observed. The developed RP-HPLC method estimated 0.465%w/w Bergenin in the roots of S. virosa and may therefore be employed in the quality control of product containing roots of S. virosa.

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GLOBAL AND LOCAL EXERCISES OF MESH REFINEMENT OF CONTROL VOLUME MODELS WITHOUT REMESHING

Liquid-water is introduced at different temperature from side and upstream in a tee pipeline domain. The operation is simulated after construction of control volume and triangular tetrahedral mesh generations are performed. Smaller global fine remeshing hung the work station and for this case, without remeshing, the grid is refined locally at jet region. Effects of the region adaption are shown and swapping is executed to improve computation. Profiles along centerline of side jet and profiles along the centerline of the pipe, away from the jet, are almost identical, however, for the jet zones the profiles showed certain differentiation. An increase in the number of cells results in a larger increase in the unit time required for convergence from machine to machine. The use of the finest refinement is recommended at the gradients.


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Friday, January 3, 2020

STORING RDF DATA: A BRIEF SURVEY

There is an actual need to efficiently store and retrieve RDF data like the number and scale of Semantic Web in real-word applications in practice growth. As datasets are linked together, scalability becomes more critical. Efficient data storage and query processing that can scale to large amounts of possibly schema-less data have become an important research topic. The Study objective is to highlight A classification of RDF storage approach; and illustrations a view of two types of RDF data stores (Traditional database stores, NoSQL database stores) And therefore, this paper gives a brief overview of storing RDF data.


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FUZZY INTERFACE OF CALCIUM CARBONATE PRODUCTION DEPICTING GAS GAUGE

Calcium carbonate is an active component of the limestone. Calcium Carbonate Equivalent and the Effective Calcium Carbonate Equivalent give a numeric to the effectiveness of different liming materials. The first method compares a particular quarry's stone with the neutralizing power of pure calcium carbonate [1]. In this paper, the process of mixing calcium hydroxide into deionized water and jet bubbling carbon dioxide gas reacting through the contained is used. Output reaction conversion on inputs: acidity, reaction time, stirring, pressure and temperature is interfaced creating a fuzzy interfacing model. Reaction conversion is implicated on inputs of acidity and reaction time for a temperature value keeping other variables constant and assuming homogeneous reaction throughout within reactor. Gas flow gauge is fall timely and considering parametric and variability change, this is depicted.


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Thursday, January 2, 2020

CIVIL ENGINEERS KNOWLEDGE OF HSE AND PROJECT MANAGERS' EXPECTATION IN OIL INDUSTRY IN IRAQ

Oil and gas industry is exposed to high risk of HSE compliance issues. The industry is highly risky by its nature, which include complex operations and procedures. Health, safety and environment (HSE) knowledge is vital requirement for employees to enrol themselves in oil and gas industry. This project aims to find the level of HSE knowledge of the Iraqi novice civil engineers working in Iraqi oil and gas industry and compare it with senior engineers and aims to identify the project managers’ expectations regarding HSE knowledge for the Iraqi novice civil engineers to determine the gap between the knowledge of novice civil engineers on HSE and expectations of project managers on novice civil engineers at Iraqi oil and gas industry. From the analysis, it was deduced that novice civil engineers are having moderate knowledge on the HSE elements of oil and gas industry. Lowest RII was observed for hazard analysis, prevention and safety. Comparison of senior and novice civil engineers revealed that there is significant gap between their knowledge levels of HSE elements. Interview approach showed that project managers expect novice civil engineers to have basic awareness, knowledge and skills related to the industry safety. Gaps were observed in terms of novice civil engineer knowledge on basic HSE elements and their accountability in sustaining a safety environment.


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Wednesday, January 1, 2020

DIVERSITY AND ETHNOMEDICINAL IMPORTANCE OF INDIGENOUS TREE SPECIES IN BAGALE HILLS FOREST RESERVE, ADAMAWA STATE, NIGERIA

The study assessed the diversity of the indigenous tree species and also produce of a compendium of information on the various medicinal uses of the indigenous tree species and their parts in Bagale Hill Forest Reserve, Girei Local Government of Adamawa State, Nigeria. Sample plots method was used in the assessment of the indigenous tree species using the simple random sampling technique. Five plots measuring 50 m x 50 m were selected within the study site. All matured trees within the plots were identified and a total count of them was carried out. Information on ethonomedicinal uses of indigenous trees was obtained through oral interviews Focus Group Discussion (FGD). Five (5) communities out of Fifteen (15) were randomly selected for this purpose. Result of the findings indicated that 38 indigenous trees species belonging to 15 families were encountered in all the five (5) plots inventoried in the study site. Results further indicate that Hexalobus monopetalus, Piliostigma thonningii, Prosopis africana, Acacia ataxacantha and Grewia molis were common to all the sites. Annona senegalensis, Detarrium microcarpum, Terminalia glaucescens, Hymenocardia acida and Ficus sycomorus were common to four site in the study area while all other species were found in either three, two or one area of the study site. Shannon Diversity Index 2.709, 3.124, 2.614, 2.551 and 2.958 respectively for sites A to E, Simpson Diversity Index stood at 0.925, 0.9467, 0.908, 0.9091 and 0.9304, Dominance stood at 0.075, 0.05332, 0.9201, 0.09091 and 0.0696, Evenness index were 0.8835, 0.7841, 0.8028, 0.8546 and 0.7706 while Equitability Index stood at 0.9563, 0.9278, 0.9225, 0.942 and 0.9191 respectively. The various families encountered were; Anacaediaceae, Annonaceae, Balanitaceae, Bombacaceae, Burseraceae, Caesalpinioideae, Capparaceae, Combretaceae, Flacourtiaceae, Hymenocardiaceae, Mimosoideae, Moraceae, Sapotaceae, Tiliaceae and Verbenaceae. The plant parts used for medicinal purposes are roots, barks, leaves, stems, Epiphyte etc. The formulation for medication include; boiling, soaking, etc. Methods of administration include, drinking, bathing, adding to food as spices and chewing. Among the ailments treated are dysentery, diarrhea, diabetes, Tuberculosis, Meningities, Blood tonic, High blood Pressure, Worms, Typhoid Fever, Cancer, Malnutrition, Tumors, Wound Healing, Jaundice, Epilepsy and Rheumatism. It can be thus be concluded that the sharp decrease in biological tree species across the globe and the increasing economic values placed on medicinal plants, documentation on ethnobatanical knowledge is a way to understand the use of different plant species to cure various ailments and means to conserve these natural resources. From the findings of the study the following recommendations are hereby made; the local people need to be trained, encouraged and supported on how to conserve and manage the medicinal plant species, Young generation need awareness to avoid negative impacts on the medicinal plants and associated knowledge in the area, hence, documentation of the medicinal plants of the area needs to continue.

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