Showing posts with label antimicrobial activity.. Show all posts
Showing posts with label antimicrobial activity.. Show all posts

Thursday, January 21, 2021

ANTIFUNGAL AND ANTIBACTERIAL EFFECTS OF CORIANDER OLEORESIN MEDIATED SILVER NANOPARTICLES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Goal: To test the antibacterial and antifungal activity of silver nanoparticles mediated by coriander oleoresin.

Materials and Methods: Silver nanoparticles mediated by coriander oleoresin were initially prepared and validated using UV-Visible Spectroscopy. The prepared silver nanoparticles mediated by Coriander oleoresin were further evaluated by standard procedure against S for their antimicrobial properties. Lactobacillus, S. Mutans, S. Aureus, C. Albicans.

Results and Discussion: The study demonstrated peak UV-Visible spectroscopy at 420 nm, suggesting synthesis of silver nanoparticles. Dose-dependent antibacterial activity with S was demonstrated. With aureus and S. Muthans, mutans. S. Coriander oleoresin-mediated silver nanoparticles with a maximum inhibition zone of 22 mm at their peak concentration (150μL) were very sensitive to aureus. At all concentrations used, Lactobacillus showed the same zone of inhibition. C. Albicans, at their maximum concentration (150 μL) of nanoparticles, have the highest inhibition zone (20 mm).

Conclusion: This study concludes that silver nanoparticles induced by Coriander oleoresin have strong antimicrobial activity and can be used for infections caused by S. Mutans, S. Mutans Aureus, C. Albicans. Albicans.

Please see the link :-
https://www.ikprress.org/index.php/PCBMB/article/view/5370

Saturday, November 21, 2020

PHYTOCHEMICAL SCREENING, ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF Semecarpus prainii King. LEAF EXTRACTS | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 For phytochemical examination, antioxidant and antimicrobial activity in petroleum ether, chloroform and ethanol extracts were subjected to Semecarpus prainii leaf extract in the present research. The existence of all the components except saponins in ethanol extract was revealed by phytochemical analysis. Saponins, anthroquinones, glycosides were absent in chloroform and petroleum ether extract, oils and fats were absent in chloroform extract, but were present in petroleum ether extract, among the tested phytochemicals. The antimicrobial activity findings showed that S. With aureus, L. About monocytogenes, E. Klebsiella sp, coli. Pseudomonas aeruginosa and Pseudomonas aeruginosa were immune to all extracts tested. Maximal inhibiton zone against M was shown by ethanol extract. (12.7 mm) luteus, Shigella sp. (12.3 millimetres) and E. Faecalis, which was almost identical to the normal antibiotic used (14.5 mm). Various techniques such as total phenolic assay, total antioxidant assay, power reduction assay, DPPH assay, ABTS and nitric oxide scavenging activity were evaluated for the antioxidant activity of leaf extracts. Important results were shown by all the tested techniques. The overall phenolic content was found to be 47.69, 98.69 and 118.36 mg-g gallic acid equivalents, respectively, of petroleum ether, chloroform and ethanol extracts. The ethanol extract displayed substantial total antioxidant activity of 501 µg of GAE accompanied by chloroform (292 µg of GAE) and petroleum ether extract (84 µg of GAE). Reducing power assay of the checked plant was in order of ethanol > chloroform > petroleum ether. At 1000 μg-mL concentrations measured, the ethanol extract showed a maximum absorbance of 2.88. Radical scavenging activity (percent) was expressed in the DPPH, ABTS and nitric oxide assay of petroleum ether, chloroform and ethanol extracts, and the results obtained showed that ethanol extract was more potential than petroleum ether and chloroform. These experimental results would help provide the theoretical foundation for conventional knowledge of the use of plants to control various diseases.


Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5250