PHYTOCHEMICAL SCREENING, ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF Semecarpus prainii King. LEAF EXTRACTS | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 For phytochemical examination, antioxidant and antimicrobial activity in petroleum ether, chloroform and ethanol extracts were subjected to Semecarpus prainii leaf extract in the present research. The existence of all the components except saponins in ethanol extract was revealed by phytochemical analysis. Saponins, anthroquinones, glycosides were absent in chloroform and petroleum ether extract, oils and fats were absent in chloroform extract, but were present in petroleum ether extract, among the tested phytochemicals. The antimicrobial activity findings showed that S. With aureus, L. About monocytogenes, E. Klebsiella sp, coli. Pseudomonas aeruginosa and Pseudomonas aeruginosa were immune to all extracts tested. Maximal inhibiton zone against M was shown by ethanol extract. (12.7 mm) luteus, Shigella sp. (12.3 millimetres) and E. Faecalis, which was almost identical to the normal antibiotic used (14.5 mm). Various techniques such as total phenolic assay, total antioxidant assay, power reduction assay, DPPH assay, ABTS and nitric oxide scavenging activity were evaluated for the antioxidant activity of leaf extracts. Important results were shown by all the tested techniques. The overall phenolic content was found to be 47.69, 98.69 and 118.36 mg-g gallic acid equivalents, respectively, of petroleum ether, chloroform and ethanol extracts. The ethanol extract displayed substantial total antioxidant activity of 501 µg of GAE accompanied by chloroform (292 µg of GAE) and petroleum ether extract (84 µg of GAE). Reducing power assay of the checked plant was in order of ethanol > chloroform > petroleum ether. At 1000 μg-mL concentrations measured, the ethanol extract showed a maximum absorbance of 2.88. Radical scavenging activity (percent) was expressed in the DPPH, ABTS and nitric oxide assay of petroleum ether, chloroform and ethanol extracts, and the results obtained showed that ethanol extract was more potential than petroleum ether and chloroform. These experimental results would help provide the theoretical foundation for conventional knowledge of the use of plants to control various diseases.

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