Over the few years,
there is an increased interest in the microbial enzymes to overcome its
inability to meet the current and future requirements of the World. In the
search of a kind, sucrase has a vital role to play with its variety of
applications, particularly in the food and biofuel productions. In addition to
their vast and varied applications, newer microbes are to be screened for
sucrase production with their desirable properties. There are two saccharolytic
enzymes induced by sucrose in Bacillus species which is encoded by SacB and
SacA in the an extracellular levansucrase and an intracellular, respectively.
In this experimental analysis, Plackett Burman Design was used for screening of
nutrients for sucrase production by Bacillus subtilis. Sucrase activity was
optimized by Plackett Burman design in Production Medium and then purified by
column chromatography. Using MINITAB 15 Software, Sucrose, Yeast Extract, and
Ferrous Sulphate had major source influence on sucrase activity compared to
other components. In column purification, maximum amount of enzymes was
obtained from the concentration of 0.5M NaCl-eluted sample.
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