Simmondsia chinensis (Family-Simmondsiaceae) is an industrial shrub of high economic importance, commercially exploited for its liquid-wax extracted from the seeds. Mass production of female plants is unavoidable to solve the problem associated with the male biassed ratio in a region. The efficient production, exchange of germplasm and distribution of female plants will be provided by synthetic seeds. The Efficient Protocol for the Production of Synthetic Seeds in S. Chinensis was developed using axillary bud encapsulation. For synseed development, axillary buds from existing cultures using Murashige and Skoog (MS) medium nodal explants supplemented with 2.0 mg/l Benzyl aminopurine (BAP) and 1.0 mg/l Naphthalene acetic acid (NAA) plus 40 mg/l Adenine sulphate (AdS) were used. In synthetic seeds produced with 3.0 percent sodium alginate and 100 mM Calcium chloride with 40 g/l sucrose in MS medium, the plantlet conversion efficiency was highest. The earliest bud initiation (9.64 days) with the maximum number of shoots per explant (4.64 shoots) and shoot length were provided by this combination (3.95 cm). Inoculated on a 1⁄2 intensity MS medium with 2 mg/l IBA and 200 mg/l activated charcoal, microshoots derived from synseed culture gave the best rooting response with 3.92 roots per plant and 3.17 cm root length. The regenerated plantlets from encapsulated beads showed a survival rate of 80 percent. This is the first study of synthetic seeds using female S' axillary buds. Chinensis for propagation by mass.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5274
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