Introduction: Symploccocus racemosa plants are very useful in various aspects of pharmacology. The copper nanoparticles are smaller in size, providing the microbes with a wide surface area for interaction. The CuNPs were being tested by SEM, TEM for finding the copper nano particles. To assess the activity of the CuNPs on Streptococcus mutans, anti-bacterial activity was carried out.
Materials and methods: preparation of the root extract: fresh lodhra bark was extracted and ground until the fine powder was made. They mixed 1 g of this powder with 100 ml of distilled water. The extract was boiled in the heating mantle for 3 to 5 minutes and was filtered.
CuNP preparation: 0.861 g of copper sulphate powder was added and added to 50 ml of distilled water; the extract of the lodhra bark was added. In a shaker, the solution was held and the readings were taken.
Antibacterial activity: on the surface of the Muller Hington agar plates, fresh bacterial suspension has been dispersed. The different concentrations of CuNPs were incorporated into the wells and incubated for 24 hours at 37 degrees Celsius.
Result and Discussion: On each plate, the diameter of the inhibition zone was registered. The change in colour was suggestive of CuNP formation and was further confirmed by the UV-vis spectrophotometer.
Conclusion: CuNPs mediated with sensitizedlodhra bark showed high antibacterial activity against S. Among other oral pathogenes, mutans. These nanoparticles can be used in further studies to cure diseases, as they can be effective in the management of sensitive microorganisms.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5339
No comments:
Post a Comment