The quality of the roots of Securinega virosa
(Euphorbiaceae) commonly marketed in Ghana for medicinal purposes needs to be
assured through standard quality control procedures. This work has isolated and
characterized a biomarker to be used in assessing the quality of products and
herbal preparations containing the roots of Securinega virosa. Using column
chromatography of 20 g ethanolic roots extract; 0.02% w/w biomarker was
obtained and characterized by melting point determination, UV-Vis, IR, 1D and
2D NMR spectroscopic analysis and comparison to reported literature. The
biomarker was identified as Bergenin. A RP-HPLC method has been developed and
validated for the quality control of S. virosa root preparations with Bergenin
as standard. The most appropriate resolution was attained by a gradient elution
of methanol: 0.05% v/v trifluoroacetic acid in water using Waters Bondapak C18
(3.9×300 mm, 5 μ) column at a flow rate of 1.0 ml/min and a wavelength of 220
nm. The retention time of the biomarker was found to be 14.20 ± 0.01 mins. The
method was subjected to ICH guidelines for validation and showed a good
linearity with a correlation coefficient (r) of 0.9993. The LOD and LOQ were
found to be 15.23 mg/L and 46.16 mg/L respectively. Tolerable levels of
accuracy, precision, specificity and robustness were observed. The developed
RP-HPLC method estimated 0.465%w/w Bergenin in the roots of S. virosa and may
therefore be employed in the quality control of product containing roots of S.
virosa.
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