In the present study,
Aspergillus costaricaensis CBS115574 which was previously isolated from the
non-leguminous field soil collected from the Kishangarh city of state Rajasthan
confirmed by 16S rRNA gene/ITS/D1-D2 region sequencing. The cultural parameters
(Carbon sources, nitrogen sources, moisture content, pH, temperature and
particle size etc) of solid-state fermentation have been optimized for the
improved extracellular lipase production. Among different carbon sources, A.
costaricaensis showed best lipase activity (253.6 IU/g/min) in the presence of
lactose. Among the various concentration (0.2%, 0.4%, 0.6%, 0.8%, 1.0%, 1.2%,
1.4% and 1.6%) of lactose, the optimum concentration was found to be 1.6%.
Among different nitrogen sources, highest lipase activity (55.32 IU/g/min) was
obtained in the presence of yeast extract and the optimum concentration of
yeast extract was found to be 0.5% for maximum lipase activity (236.69 IU/g).
Further the best lipase activity was obtained in the medium of pH 8.0 (312.330
IU/g/min) at 28ºC after 48 hrs of incubation period. Further the particle size
of solid substrate was optimized and highest lipase activity was found when
fine particle size was used. Among the various ratio of moisture content,
highest lipase activity (317.09 IU/g/min) was found at 1:6 moisture content.
From the present work, it is figure out that for the enhanced production of
lipases the medium parameters required to be optimized. The above results help
out the authors in carry out the further studies of purification and
characterization of lipase enzyme produced from A. costaricaensis CBS115574.
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