The first double
haploid lines were obtained in the anther culture more than 50 years ago, but
so far their production remains more art than a well-established technology.
There are a huge number of articles with conflicting information, both on
preliminary processing of anthers and medias that determine the highest yield
of regenerants, despite the fact that the relevance of research in this area is
difficult to overestimate. Double haploid (DH) lines are used: For increasing
efficiency of traditional breeding; obtaining populations of DH lines used in
several stages of fixing heterosis effect (to remove semi-lethal genes, as well
as homozygotization of material) and so on. The objective of the study is to
investigate the efficiency of media for producing DH lines in anther culture of
hybrids between Russian and foreign varieties. The anthers of 67 hybrid
combinations, both interspecific and intraspecific hybrids, were used. 30-50
anthers were planted on Petri dish (6 сm). After formation of callus (size varied
from 2 to 10 mm) on the medium for dedifferentiation, they were transferred to
MS medium for regeneration. Plants from 5 cm in size were transferred to the
root formation medium. We study the effectiveness of nutrient media, which are
derivatives of N6, but with a different hormonal composition; and С, RZ media,
the salt content was also changed. Medias RZ and C did not reliably differ in
callus yield; the rest were reliably inferior to them in this trait. Therefore,
to increase the yield of callus, a higher content of salts of KNO3, KH2PO4,
MgSO4 • 7H2O, MnSO4 • 4H2O, ZnSO4 • 7H2O, H3BO3, CaCl2 • 2H2O vitamins is
necessary. However, their hormonal composition is different. If the RZ medium
contains naphthylacetic (NAA) and dichlorophenoxyacetic acids ( 2,4-D), then C
is only 2,4-D. N6-based media, N6a and N6б contain only one auxin as well as C.
In the study, the best results were shown by option with content of both
hormones at a concentration 2 mg / l NAA and 1 mg/l 2,4-D. The results showed a
significant positive effect of increasing iron (FeSO4 x 7 H2O) concentration
(55.6 mg / L) both on callusogenesis and plant regeneration. In standard recipe
of all the media used in the work, its concentration is 27.8 mg / l. To
stimulate somatic embryogenesis, the media were modified also by the addition
of abscisic acid (ABA) and silver nitrate AgNO3. Studies have shown
that somatic embryogenesis is stimulated in many genotypes when using ABA in
combination with cytokinins. Callusogenesis and regeneration in the considered
combinations had maximum values at AgNO3 concentration 2 mg/l and;
1 mg/l ABA for the of most of the studied hybrids.
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