Background: Nanotechnology is a relatively new field of pharmacological research that has a wide range of applications in the biomedical, engineering, chemical, pharmaceutical, and food industries. The use of microorganisms and herbal extracts for nanoparticle synthesis has attracted international attention, and it is one of the most interesting nanotechnologies. Because of their versatility, silver nanoparticles have a lot of potential in biomedical research. Silver has been found in many bacteria and microorganisms, and it has been discovered that it inhibits their growth and existence. In plant biotechnology, green synthetic techniques for the development of nanoparticles have recently sparked a lot of interest. Biologically synthesised silver nanoparticles are more beneficial than chemical reductions because they are eco-friendly, low-cost, and one-stage processes. Tulsi, chitharathai, adathoda, turmeric, and other herbs found in the Indian subcontinent have various medicinal values and have been used in traditional medicines and home remedies, such as tulsi, chitharathai, adathoda, and turmeric. Justicia adhatoda (adathoda) contains photochemical compounds, and Ocimum sanctum (tulsi) contains germicide and disinfectant. Ocimum Sanctum (Tulsi) and Justicia adhatoda (adathoda), according to earlier research, have a wide range of medicinal properties, including antifungal, antiviral, antibacterial, and cytotoxic properties.
The aim of this research is to use UV Visible Spectroscopy to investigate the antioxidant and cytotoxic activity of silver nanoparticles mediated by herbal extracts of Ocimum sanctum and Justicia adhatoda.
Materials and Methods: Ocimum sanctum and Justicia adhatoda herbal extracts were prepared in our laboratory from a preexisting powder to achieve green synthesis of silver nanoparticles. The physical characterization of these silver nanoparticles, such as size and shape, was confirmed using UV-visible spectroscopy, and the cytotoxic and antioxidant activity was tested and characterised using UV-visible spectroscopy.
At 450 nm, the surface plasmon resonance band (SPR) was measured. The antioxidant activity of nanoparticles increased as concentration was increased, and cytotoxic activity was measured using the number of live shrimps, which decreased as concentration was increased. The percentage inhibition of antioxidant activity was 50 mu/L, and the percentage inhibition of cytotoxic activity was 25 mu/L.
Conclusion: Silver nanoparticles made from Ocimum Sanctum (Tulsi) and Justicia adhatoda (adathoda) were biosynthesised in a novel way and showed significant antioxidant and cytotoxic activity with minimal side effects. This is the first report of Tulsi and adhatoda-mediated silver nanoparticle synthesis with such properties, which will need to be investigated further using other characterization and testing methods.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5566
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