The acclimatisation stage of date palm plantlets is critical for increasing the number of plants to culture in the open field, so this study was done to improve plantlets in the rooting stage in vitro by using different concentrations of KH2PO4 as full (170 mg L-1), full (170 mg L-1) + 14, and full (170 mg L-1) + 12 and sucrose 30 as a growth medium. The results revealed that treating KH2PO4 at full strength with 45 g L-1 sucrose was an effective treatment for increasing in vitro plant height. Although root duration, number, and stem thickness increased significantly under KH2PO4 full + 12 with 60 g L-1 sucrose, full KH2PO4 +30 g L-1 sucrose produced the most hairy roots, full KH2PO4 +30 g L-1 sucrose produced the most leaves, and full KH2PO4 +14 with 45 g L-1 sucrose produced the highest percentage of plantlets acclimatisation. Under complete +1/4 and full + 12 KH2PO4 with 45 g L-1 sucrose, more total indoles, chlorophyll a and b were present. Based on the previous findings, full + 14 or full + 12 KH2PO4 treatment with 45 g L-1 sucrose is preferred for active in vitro and ex vitro stages.
Please see the link :- https://www.ikprress.org/index.php/JOBARI/article/view/5672
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