The current research focuses on isolating actinomycetes from Vitis vinifera L. and Annona squamosa L. fruits, as well as screening for bioactive compounds released by actinomycetes, such as antibacterial and amylase inhibitory activity, and their role in fruit quality.
Pretreatment and growth on selective nutrient medium without and with antibiotics were used to isolate the actinomycetes from the fruit. Antimicrobial activity of isolate extracts was screened using an antibiotic disc diffusion technique, and amylase inhibition activity was screened using a spectroscopic approach using 3, 5-dinitrosalicylic acid.
Using calcium carbonate, dry heat pretreatment, and growth on medium with and without antibiotics, a total of 16 actinomycetes were isolated from Vitis vinifera L. and Annona squamosa L. fruits samples. Antibacterial activity against Escherichia coli and Pseudomonas aeruginosa was detected in 5 of the 16 isolate extracts. Extracts from isolates G2 and C2 inhibited amylase by 30 and 50 percent, respectively.
Conclusion: Actinomycetes are found on the fruits of Vitis vinifera L. and Annona squamosa L., and they produce antibacterial activity that helps restore fruit quality by preventing unwanted bacterial growth. Isolated actinomycetes were discovered to be a source of bioactive compounds and amylase inhibitory activity, both of which help to prevent starch degradation.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5565
No comments:
Post a Comment