Candida species-induced vulvovaginitis is a widespread fungal infection among adult and pregnant women. Vulvovaginal candidiasis is caused by Candida albicans (VVC). The participants in the study were 250 women ranging in age from 17 to 45 years old who were attending specialist hospitals in Baghdad, Iraq. Candida spp. was detected using standard methods and biochemical tests after vaginal smears were cultured on selective chrome agar and Sabouraud dextrose agar. Polymerase Chain Reaction (PCR) was also used to identify isolates, with the universal ITS4 area primer and species-specific primers. There were 141 (56.4%) yeast isolates from the 250 vaginal smears obtained, with 81 (57.4%) Candida albicans and 60 (42.6%) non-albicans. Candida albicans was the most common Candida species (57.4 percent), followed by C. glabrata (20.6 percent), C. tropicalis (13.5 percent), and C. krusei (12.5%). Candida spp. were identified by PCR, which verified the primary identification by biochemical tests. The internal transcript spacer (ITS) 1 and 2 primers produced amplification products of 402 bp in the PCR assays for C. albicans in the examined samples and a high sensitivity to molecular detection for Candida species other than albicans among the local isolates. Finally, the high prevalence of Candida isolates among Iraqi female Vulvovaginitis patients highlights the importance of frequent candidiasis screening and evaluation in Iraqi hospitals.
Please see the link :- https://www.ikprress.org/index.php/JODAGH/article/view/5655
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