CYTOTOXICITY STUDY OF COPPER OXIDE NANOPARTICLE MEDIATED THROUGH TAMARIND EXTRACT | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Context: Plant-based nanoparticles are stated to be healthy and eco-friendly.

Objective: To classify the cytotoxic effect of copper oxide nanoparticles mediated by tamarind fruit pulp on brine shrimp shrimp

Materials and methods: 0.55 g of tamarind extract was combined with 100 ml of distilled water to obtain a transparent solution to which 0.318 g of copper sulphate was added and stored for nanoparticle synthesis in an orbital shaker. The absorbance was measured by UV-Visible Spectroscopy at regular intervals and centrifuged and stored after verifying the formation of the nanoparticles. A plate of 6 wells of Elisa was taken and filled with saline water and 10 brine shrimp nauplii were put with due care in each well. In each well, different volumes of nanoparticles starting from 5 μl to 25 μl were taken and the cytotoxicity was tested 24 hours later.

Results and Discussion: The number of nauplii brine shrimps was counted and all of the nauplii on the first day were alive. On day 2, only 9 nauplii were detected alive at 5 μL and 10 μL concentrations; 7 were detected alive at 15 μL and 20 μL concentrations; and 6 nauplii were detected alive at 25 μL concentrations. The overall cytotoxicity was observed at a concentration of 25μl. All the nauplii were alive in the control group without the copper nanoparticle controlled by tamarind.

Conclusion: The current research concludes that nanoparticles of copper oxide are healthy at low concentrations. After more in-vivo research, the dose dependent cytotoxicity found with tamarind-mediated copper nanoparticles may be used for use in cancer therapeutics.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5351