Chrysanthemum indicum MEDIATED SELENIUM NANOPARTICLES AND ITS ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY ABSTRACT | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Introduction: Biological nanoparticle synthesis has risen in popularity in recent years due to its cost-effectiveness, abundant raw material sources, low toxicity, and pharmacological potential. The ability to synthesise SeNPs using bacteria and plants indicates that they may be useful in a number of fields. The aerial sections (stems, leaves, and flowers) of Chrysanthemum indicum have been used in Oriental herbal medicine to treat vertigo, hypertensive symptoms, and a number of infectious diseases.

Materials and Methods: Distilled water was mixed with 1 mL of Chrysanthemum indicum extract. The mixture was heated for 15 to 20 minutes at 60 degrees Celsius before being filtered. For the synthesis of nanoparticles, 0.519 grammes of Selenium were applied to the filtered extract and alternated between an orbital shaker and a magnetic stirrer. To assess the antioxidant activity of Chrysanthemum indicum mediated selenium nanoparticles, a DPPH radical scavenging assay was used. The anti-inflammatory activity of Chrysanthemum indicum mediated selenium nanoparticles was assessed using an albumin denaturation inhibition assay. The reagent for the assay was BSA (Bovine serum albumin).

Results: The antioxidant and anti-inflammatory activity of five different concentrations of reaction mixture were tested in this study: 10 L, 20 L, 30 L, 40 L, and 50 L. Plant extract induced by selenium nanoparticles at a concentration of 50 litres had the highest antioxidant activity and anti-inflammatory activity, both of which were inhibited by 87 percent.

Conclusion: In both assays, the antioxidant and anti-inflammatory activity of Chrysanthemum indicum mediated selenium nanoparticles was effective.

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