GREEN SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLE USING Phyllanthus emblica AND Cinnamomum verum EXTRACT | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

 Phyllanthus emblica is a small to medium-sized tree that produces greenish yellow fruits. Vitamin C is abundant in Phyllanthus emblica. It has been shown to be beneficial in the treatment of toothache, gingival inflammation, and other forms of ulcers due to its high antioxidant content. Due to their peculiar properties such as surface enhanced scattering and thermal conductivity, silver nanoparticles (AgNPs) have emerged as one of the most promising nanoparticles. Nanotechnology refers to a wide range of technologies, methods, and processes that deal with matter at the nanoscale, which ranges from 1 nanometer to 100 nanometers in size. The aim of the research is to make silver nanoparticles from Phyllanthus emblica and Cinnamomum verum and characterise their properties using a transmission electron microscope.

Materials and Methods: The herbal treatment centre provided Phyllanthus emblica fruit extract and Cinnamomum verum powder. A conical flask containing 50 ml of distilled water was filled with 0.5 grammes of both extracts. The extract was then heated at 60°C for 7-8 minutes using a heating mantle, and 10 ml of the plant extract was mixed with 90 ml distilled water, along with 0.0169 grammes of silver nitrate, and put in a shaker for uniform distribution. The colour change was observed every hour for two days. The nanoparticle was characterised using the TEM analysis process.

The effect of varying reaction parameters such as temperature, PH, and reactant concentration on the synthesis of silver nanoparticles using Amla and cinnamon fruit extract was investigated using UV-visible spectroscopy of AgNPs. This method produces stable, spherical silver nanoparticles with sizes ranging from 5 to 45 nanometers.

Conclusion: Using an Amla and cinnamon formulation, the current study was effective in synthesising plant-mediated silver nanoparticles. The majority of the silver nanoparticles synthesised were spherical in shape and ranged in size from 5-45 nm.

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