An successful method for the conservation of valuable plants has been provided by in vitro propagation. The objective of the investigation was to identify the effect of the various growth regulators on the propagation of Scoparia dulcis L in vitro. Usage of leaves, nodes and internodes as explants in Murashige and Skoog (M.S.) media. The research was conducted in 2017 at the Plant Tissue Culture Laboratory, Department of Botany, Patna Science College, Patna. During the research project, M.S. Fortified media with different combinations and concentrations of 2,4-D, IAA, BAP+2,4-D for callogenesis and BAP+IAA, BAP+NAA for shoot induction and individual IBA and NAA for root formation in both solid and liquid media were used. During the analysis, it was found out that callogenesis was best done in leaf explants. The overall average number of shoots was produced from callus at 1.5mg/l BAP+0.5mg/l IAA. The maximum average number of roots was generated at 0.5mg/l NAA in half strength liquid media. The regenerated plantlet survival rate was 85 percent . The investigation will also help to develop an efficient protocol for the protection of the ruderal weed in vitro- Scoparia dulcis L.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5537
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