Background: Application of chitosan in dentifrices or mouthwash is limited, several studies have shown that it is extremely effective in destroying bacteria as well as reducing plaque film and gingivitis. Lycopene can function as a potent scavenger of Hypochlorous acid at a wide range of concentration that may span various pathophysiological and supplemental ranges. Therefore combining both using nanotechnology would be beneficial for the exploitation of new generations of antimicrobial agents and for the development of new biomedicine.
Aim: The
study aims to prepare lycopene mediated chitosan based mouthwash and to assess
its antimicrobial and cytotoxic effects.
Materials and
Methods: The study involved the preparation of lycopene mediated chitosan based
mouthwash from their extracts. Assessment of cytotoxic and antimicrobial
effects of mouthwash was done. In order to test its cytotoxicity a primary
level animal testing on brine shrimps was conducted using the standard brine
shrimp lethality assay (BSLA) method and agar diffusion method was used to
assess its antimicrobial properties.
Results: The
study reveals that the mouthwash prepared had better antimicrobial properties
while evaluating it by agar diffusion methods and cytotoxicity levels which
were calculated in relation to the mortality rates of the BSLA are directly
proportional to the concentration of the nanoparticle used. This gives an
insight into the safety and drug dosage levels.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5330
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