The LC50 value indicates the genotypic sensitivity of a species in the chemical mutagenesis experiment, which promotes the fixation of appropriate mutagen concentrations to achieve a higher rate of mutations with nominal population damage. The present study was carried out in two separate stages, initially calculating the lethal concentration (LC50) of EMS and DES and finally quantitatively analysing the mutations induced by the established concentrations for the yield characteristics of the indigenous maize germplasm of Mizoram. Analysis of the probit curve based on the germination percentage of treated maize seeds showed that EMS and DES LC50 values were 117.49 mM and 138.04 mM, respectively. Observations on quantitative characteristics have shown that LC50 therapies have successfully induced substantial beneficial genetic variations, including overall average grain (93.41 g) and stover (198.16 g) yield increase in 100 mM EMS therapy. The results indicated that the efficacy of EMS in the induction of useful micro-mutations was relatively higher than that of DES at their respective optimum maize germplasm concentrations in the current study. In conclusion, the study verified and recommends the use of EMS and DES concentrations up to an optimum of 100 mM and 150 mM respectively to produce a large range of quantitative yield mutations that could be useful in potential Mizoram indigenous maize germplasm mutation breeding programmes.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5484
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