HING OIL MEDIATED SYNTHESIS OF SILVER NANOPARTICLES, CHARACTERISATION AND ITS ANTIMICROBIAL ACTIVITY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Aim: To prepare silver nanoparticles from hing oil and to test their antimicrobial activity was the objective of the research.

Materials and Methods: In a conical flask 0.5 mL of hing oil was mixed with 99.5 mL of distilled water. To this, 0.0169 grams of silver nitrate was added and kept in an orbital shaker for the formation of nanoparticle. The colour change was noted visually and the absorbance was noted using UV -Vis spectroscopy. The final product was centrifuged and NPs were collected. The morphology was studied using TEM. The hing oil mediated silver nanoparticles were evaluated for its antimicrobial activity by agar well diffusion method using standard procedure. The antimicrobial activity was tested against S. aureus, S. mutans and Lactobacillus and Candida albicans.

Results and Discussion: The formation of silver nanoparticles at a peak of 420 nm was confirmed by UV-Vis spectroscopy. Spherical and rod-shaped nanoparticles with a size ranging from 10-55 nm were shown by TEM. The antibacterial and antifungal activity of the silver nanoparticles induced by the hing oil was comparable. With an increase in concentration, there was no difference in the inhibition region.

Conclusion: Silver nanoparticles mediated by Hing Oil may be a safer option for infections caused by bacteria like S. aureus, S. Although the zone of inhibition shown was similar in the different concentrations used in the analysis, mutans and Lactobacillus and fungi such as Candida albicans were similar. However, for their further pharmacological function, hing oil mediated green synthesis of silver nanoparticle was successful and can be further explored. For its other biomedical uses, Hing oil mediated silver nanoparticles can be assessed.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5342