GRAPE SEED AQUA ALCOHOLIC EXTRACT PREPARATION AND ITS ANTIOXIDANT, ANTIMICROBIAL AND CYTOTOXIC EFFECT - AN in vitro STUDY | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Background: Oral disorders, such as dental caries, periodontitis and oral mucosal lesions, are considered to be the most prevalent public health issues worldwide and have a highly important impact on overall health and quality of life.

Objective: To prepare a grape seed aqua-alcoholic extract and analyse its antimicrobial, antioxidant and cytotoxic impact.

Materials and Methods: Red grape seeds were dried and powdered, and water and ethanol were combined with the powdered grape seeds. The aqua alcoholic extract thus obtained was then subjected to a DPPH assay to analyse its antioxidant effect and was loaded into the agar wells developed on Muller Hinton agar in order to test its antimicrobial activity using a well diffusion process. The zone of inhibition was noted against Streptococcus mutans, Lactobacillus and Candida albicans. By using brined shrimp nauplii, the extract was then tested for cytotoxicity.

Results and Discussion: When subjected to a DPPH assay to search for its antioxidant activity at a wavelength of 517 nm, the aqua alcoholic grape seed extract displayed the highest absorbance at a concentration of 50 μl (90.9 percent). The inhibition zone for the prepared extract against Streptococcus mutans was found to be 24 mm, 30 mm and 40 mm at concentrations of 50, 100 and 150 μl, respectively. Similarly, the region of inhibition was found to be 6 mm, 8 mm, and 12 mm for Lactobacillus. The zone inhibition values for Candida albicans were 6 mm, 8 mm and 14 mm, respectively. At 10 μl, the cytotoxicity of the extract was the lowest.

Conclusion: The prepared grape seed extract has proven to be an effective antioxidant and antimicrobial agent and can therefore be used for medical applications. The cytotoxicity assay showed that the extract can be integrated into biomedical applications, thereby concluding that the extract of grape seed can be a groundbreaking step towards the transition to greener medicine.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5349