Background: In the past few years, there has been an increase in periodontal disorder, which can be due to different causes. The treatment modality, however, remains the same, which is to recognise the cause of the disease and remove the cause of the oral cavity to create a safe microenvironment to repair itself to its full ability. The use of nanotechnology has become increasingly common in different dentistry fields.
Aim: The research aims to test the cytotoxic impact of chitosan nanoparticles incorporated with lycopene using a brine shrimp lethality assay.Materials and Methods: The analysis included the assessment of the ability of lycopene-integrated chitosan nanoparticles ranging from 5 (μl - microlitre) to 50 μl as an emerging dental nanomedicine at different concentrations. A primary level animal test on brine shrimps was performed using the standard brine shrimp lethality assay (BSLA) method in order to test its cytotoxicity.
Results: The analysis shows that the levels of cytotoxicity (calculated in relation to the BSLA mortality rates) are directly proportional to the nanoparticle concentration used. It was noted that in all concentrations except 50 μl, lycopene has comparatively low cytotoxic levels, chitosan has a high cytotoxic level of more than 5 μl and the nanoparticle compound also has a high cytotoxicity of 5 μl. Another result from the analysis is that as compared to the compound particle, the individual components of the nanoparticles exhibited much less toxicity.
Conclusion: The analysis showed that the nanoparticle compound exhibits minimal toxicity at concentrations below 5 microL, but substantial nanoparticle toxicity occurs at higher concentrations. This provides an insight into the drug's secure dosage calculations. To ensure its protection and clinical applications, further research is required.
Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5368
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