GREEN SYNTHESIS OF GRAPE SEED OIL MEDIATED SILVER NANOPARTICLE AND PREPARATION OF GEL- FOR PERIODONTAL DISEASES | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Objective: The purpose of the current study was to develop a gel based on silver-mediated grape seed oil for the treatment of periodontal diseases.

Materials and methods: The preparation of grape seed oil gel consists of two stages: Stage 1: the synthesis of silver nanoparticles using grape seed oil; the addition of 10 ml of distilled water to 1 ml of grape seed oil and the addition of 1 milli mol of silver nitrate to 90 ml of distilled water, all of which are blended together and put over a magnetic agitator. The concentrate obtained was subjected to toxicity tests using brine shrimp larvae, UV spectrometry, and TEM scanning for the antimicrobial test zone of inhibition assessed for Candida albicans and lactobacillus. Step 2: carbopol was taken in a beaker and dispersed in 50 ml of distilled water after carbopol was dispersed. Before the consistency of the gel was obtained, the grape seed oil extract was applied under continuous stirring.

Results: Results for antimicrobial activity indicate that the antibacterial activity of the grape seed oil gel is increased at 100 Mul concentration, and the antibacterial activity rises as the concentration increases. The degree of lethal toxicity obtained was directly linked to the concentration of the oil samples tested in the cytotoxicity assay. The findings indicate that there was a yield of 99%. The TEM study reveals the surface structure of grape seed oil Ag nanoparticle that was spherical and morphology that was smooth and ranged in size from 40-80 nanometers. The gel was prepared as a final product after analysing all the above properties.

Conclusion: We were able to successfully synthesise silver nanoparticles within the limits of the study and infuse them with grape seed oil and examine the characteristics of silver nanoparticle gel mediated by grape seed oil and found that the synthesised product has antimicrobial properties, non-toxic in nature, after which the concentrate was shaped into gel. Before using this novel product as a gel in patients with periodontal diseases, further research must be performed.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5367