The aim of this study was to isolate, characterise, and evaluate total phenol, flavonoid content, and antioxidant activity of fungal endophytes isolated from Ziziphus jujuba Mill. from various locations in Burdwan, West Bengal, India. The isolation of fungal endophytes was done using potato dextrose agar (PDA) media according to the standard protocol. The standard protocol was followed for identification, colonisation frequency determination, fermentation, and extraction of the fungal endophytes. The bioactive compound's qualitative assay was investigated using normal procedures. Normal spectrophotometric methods were used to estimate total phenol and flavonoid content quantitatively. The phosphomolybdenum assay and the 2, 2- diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method were used to assess the antioxidant activity of the cell-free extract. DPPH free radical scavenging was used to evaluate the IC50 values of the fungal endophytes. Four dominant endophytic fungi from Ziziphus jujuba have been isolated and described, with the highest colonisation frequency in ZSS2 (Cladosporium sp. 1) and ZSS4 (Aspergillus sp. 1). ZSS1 (Curvularia sp. 1) and ZSS4 (Aspergillus sp. 1) were found to be positive for the synthesis of various functional metabolites studied in this analysis, according to qualitative detection of secondary metabolites. According to the results of the quantitative analysis, ZSS1 produced the most phenol and flavonoid, followed by ZSS4 and ZSS5. ZSS1 had the highest antioxidant activity in both total antioxidant potential (TAC) and free radical scavenging activity as measured by the phosphomolybdenum assay (inhibition percent ). The total phenol and flavonoid content of the fungal extract were found to have a strong positive association with the antioxidant activity of the extract. Thus, the secondary metabolites formed by endophytic fungi isolated from Ziziphus jujuba Mill. may be a potential source of novel natural antioxidant compounds, according to the current study.
Please see the link - https://www.ikprress.org/index.php/PCBMB/article/view/5668
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